Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,039 C→T 16.8% R103Q (CGG→CAG)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0390CT16.8% 378.7 / 56.6 160R103Q (CGG→CAG) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base C (70/63);  new base T (17/10);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 167 total aligned reads displayed.

CGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTAT  >  NC_000913/1343941‑1344138
                                                                                                  |                                                                                                   
cGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCg                                                                                                    >  2:822655/1‑100 (MQ=255)
cGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCg                                                                                                    >  1:2004815/1‑100 (MQ=255)
 gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTccag                                                                                                   >  1:15359/1‑98 (MQ=255)
 gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCgg                                                                                                   >  2:3528811/1‑100 (MQ=255)
    cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                                <  1:1223806/100‑1 (MQ=255)
    cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                                <  2:2338345/100‑1 (MQ=255)
     aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                                >  1:2181343/1‑99 (MQ=255)
     aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGATGCTGACCGTGATTATAGTGACGTGTTTCGTCTGCTCGCCCAGAGTCCGGGCg                                                                                                >  1:3622065/1‑99 (MQ=255)
      aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                              >  2:944690/1‑100 (MQ=255)
      aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                              >  2:1810049/1‑100 (MQ=255)
      aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                              <  1:3749121/100‑1 (MQ=255)
      aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                              <  2:2359079/100‑1 (MQ=255)
       aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc                                                                                             <  2:874327/100‑2 (MQ=255)
       aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc                                                                                             <  1:1719354/100‑2 (MQ=255)
       aaTAACTCCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGt                                                                                             <  1:2092021/100‑1 (MQ=255)
        aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgca                                                                                            <  1:1510469/100‑3 (MQ=255)
         tAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAg                                                                                           <  2:3210370/100‑1 (MQ=38)
           aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                         >  1:3798848/1‑100 (MQ=255)
           aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAg                                                                                           >  1:2484320/1‑98 (MQ=255)
            cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAgcagaag                                                                                        <  1:517440/100‑7 (MQ=255)
            cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                        >  1:764852/1‑100 (MQ=255)
            cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                        >  2:1445550/1‑100 (MQ=255)
            cTTCACAAGGCGAGTCTTGCAGCCAATGCCCGATGTAGCTGCTGACCGTGATGATAGGGACATTCTTCTTCTAGTCGCCCGGACTCTGGGCgcgcagaa                                                                                         <  1:307786/99‑8 (MQ=255)
             ttCACAAGGCGCGTCTTTCAGTAAATGCGCGTTGTAGCTGCTGACCGTGATGATAGTGACAGTTGTCTTCTACGCGCCCGTACTCTGGGCGAGCAgaaga                                                                                       <  1:4046583/100‑5 (MQ=255)
               cacaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                                     >  1:3283516/1‑100 (MQ=255)
                acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg                                                                                    <  1:4151237/100‑1 (MQ=255)
                acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg                                                                                    <  1:3222766/100‑1 (MQ=255)
                 caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                   >  1:1805524/1‑100 (MQ=255)
                      cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                              <  2:3422340/100‑1 (MQ=255)
                      cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                              <  1:3795607/100‑1 (MQ=255)
                       gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                             <  1:1796254/100‑1 (MQ=255)
                       gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                             <  1:2529641/100‑1 (MQ=255)
                         gTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCa                                                                           <  1:2981171/100‑1 (MQ=255)
                          tCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAt                                                                          <  2:3026332/100‑1 (MQ=255)
                          tCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAt                                                                          <  1:1440115/100‑1 (MQ=255)
                          tCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAt                                                                          <  1:209788/100‑1 (MQ=255)
                             ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                       >  2:2383714/1‑100 (MQ=255)
                              tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                      <  2:4225516/100‑1 (MQ=255)
                              tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                      <  2:2280937/100‑1 (MQ=255)
                              tCAGCAAATGCGCGATGTAGCTGCTGACAGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                      >  2:1121261/1‑100 (MQ=255)
                               cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCg                                                                     <  2:2030969/100‑1 (MQ=255)
                                aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                    <  2:4025163/100‑1 (MQ=255)
                                 gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                   >  2:3174846/1‑100 (MQ=255)
                                   aaaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaa                                                                 >  2:308368/1‑100 (MQ=255)
                                    aaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                                <  1:288518/100‑1 (MQ=255)
                                       gcgcGATGTAGCTGCTGACAGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAGTGCTGCTGCCATTTTCGATAAAg                                                               >  1:3562980/1‑98 (MQ=255)
                                        cgcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATg                                                            >  1:759411/1‑100 (MQ=255)
                                               tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGc                                                     >  1:2920342/1‑100 (MQ=255)
                                                aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                    <  1:3115385/100‑1 (MQ=255)
                                                   tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                                 >  2:3660312/1‑100 (MQ=255)
                                                     ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                               <  1:1852926/100‑1 (MQ=255)
                                                     ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                               >  2:2060156/1‑100 (MQ=255)
                                                     ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                               <  2:2731100/100‑1 (MQ=255)
                                                       gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa                                             >  2:2724730/1‑100 (MQ=255)
                                                         ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGGCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                           >  2:1923096/1‑100 (MQ=255)
                                                          cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGCAcc                                          >  1:1701882/1‑100 (MQ=255)
                                                          cGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                          >  2:2423503/1‑100 (MQ=255)
                                                                gatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                                    >  2:1412965/1‑100 (MQ=255)
                                                                gatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                                    <  2:2826848/100‑1 (MQ=255)
                                                                   aGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                                 >  1:630946/1‑100 (MQ=255)
                                                                      gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCa                              <  2:3818528/100‑1 (MQ=255)
                                                                        cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                            >  2:2053396/1‑100 (MQ=255)
                                                                        cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                            >  2:351900/1‑100 (MQ=255)
                                                                        cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                            >  1:1044297/1‑100 (MQ=255)
                                                                         aTTTTTCTTCTGCTCGCCCAGAGTCCGGGTGAGTAGGGCATTGCTGCTGCCATTTTCTATACAGATGGTTTCGCCCGTTTGAACCAGCGACGCGGCaaa                            <  1:2281213/99‑1 (MQ=255)
                                                                           ttttCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCagcaaa                         >  2:3600111/1‑95 (MQ=255)
                                                                               gccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                     >  2:3312816/7‑100 (MQ=38)
                                                                               cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                      <  1:932830/99‑1 (MQ=255)
                                                                               cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                     <  2:1785859/100‑1 (MQ=255)
                                                                               cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGACATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                      >  2:1336196/1‑99 (MQ=255)
                                                                                ccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                    >  1:2438683/6‑100 (MQ=38)
                                                                                ccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                    >  2:3980248/6‑100 (MQ=38)
                                                                                ccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                     >  1:2377749/6‑99 (MQ=37)
                                                                                ccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                    >  1:3362702/6‑100 (MQ=38)
                                                                                 tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                   >  1:119183/1‑100 (MQ=255)
                                                                                 cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                   >  2:1037210/5‑100 (MQ=255)
                                                                                 cctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                   >  1:1519396/5‑100 (MQ=38)
                                                                                  ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGTCAAACTCGGCGAGt                  >  1:2910449/4‑100 (MQ=255)
                                                                                  ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt                  >  2:594992/4‑100 (MQ=255)
                                                                                   tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt                 <  2:2973819/100‑1 (MQ=255)
                                                                                   tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt                 <  1:112274/100‑1 (MQ=255)
                                                                                   tGCTCGCCCAGAGTCCGGGCGAGTAGGGCAGTGATGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAGTCGGCGAGtt                 >  1:2798965/1‑100 (MQ=255)
                                                                                    ncTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTGTCGCCCGGTTTAACCAGCGACGCGGCAAACTCGGCGAGtt                 >  2:1766615/2‑99 (MQ=255)
                                                                                    ncTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                >  2:427915/2‑100 (MQ=255)
                                                                                    gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                <  2:185869/100‑1 (MQ=255)
                                                                                    acTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                >  2:1130490/2‑100 (MQ=255)
                                                                                    acTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                >  2:1149131/2‑100 (MQ=255)
                                                                                     cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa               >  1:1650383/1‑100 (MQ=255)
                                                                                     cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGACATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt                 >  1:2286196/1‑98 (MQ=255)
                                                                                     cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa               >  2:3838050/1‑100 (MQ=255)
                                                                                         cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt           >  2:2694316/1‑100 (MQ=255)
                                                                                           cAGGGTCCGGGCAAGTAGGTCATTGCGGCTTCCATTTTCGATAAAGATAGTTTCGCCCGGTTGAACCAGCGACGCTGCAAACTCGGCGAGTTCACGCTTc         <  1:3614426/100‑1 (MQ=255)
                                                                                           cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc         <  2:764854/100‑1 (MQ=255)
                                                                                           cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc         <  1:924295/100‑1 (MQ=255)
                                                                                           cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc         <  1:1923092/100‑1 (MQ=255)
                                                                                             gtgTCCGGGAGAGTAGGGCATTGCTGATGTCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCAACACCTCAAACTCGCCGAGTGCActct           <  1:786158/94‑1 (MQ=255)
                                                                                                 ccGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCATGCTTCAGCGta   >  1:1015325/1‑100 (MQ=255)
                                                                                                 ccGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGta   >  1:2262115/1‑100 (MQ=255)
                                                                                                 ccGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGta   >  2:2406349/1‑100 (MQ=255)
                                                                                                  cGGGCTAGTAGTGCATTGCTGCTGCCATTTTCGATAAAGATGTTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGta   <  1:2987890/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
CGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTAT  >  NC_000913/1343941‑1344138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: