Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,368,184 A→T 30.9% M36L (ATG→TTG)  pspA → regulatory protein for phage‑shock‑protein operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,368,1840AT30.9% 221.1 / 118.1 148M36L (ATG→TTG) pspAregulatory protein for phage‑shock‑protein operon
Reads supporting (aligned to +/- strand):  ref base A (54/48);  new base T (22/24);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAG  >  NC_000913/1368086‑1368282
                                                                                                  |                                                                                                  
ttttttCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGAt                                                                                                   <  2:531758/100‑1 (MQ=255)
 tttttCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATg                                                                                                  >  2:1289842/1‑100 (MQ=255)
 tttttCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATg                                                                                                  >  2:3148145/1‑100 (MQ=255)
   tttCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGa                                                                                                >  1:6265/1‑100 (MQ=255)
    ttCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGaa                                                                                               >  2:4096038/1‑100 (MQ=255)
      ctcGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGa                                                                                             >  1:1655293/1‑100 (MQ=255)
       tcGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGAt                                                                                            <  2:788212/100‑1 (MQ=255)
       tcGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGAt                                                                                            <  2:603219/100‑1 (MQ=255)
       tcGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGAt                                                                                            <  2:3429364/100‑1 (MQ=255)
        ngCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATa                                                                                           >  2:275349/2‑100 (MQ=255)
          cTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATaca                                                                                         <  2:2002847/100‑1 (MQ=255)
          cTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATaca                                                                                         <  1:3327680/100‑1 (MQ=255)
            ttGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACt                                                                                       <  2:2690074/100‑1 (MQ=255)
            ttGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACt                                                                                       <  2:4174680/100‑1 (MQ=255)
              gCCGACATCGTGAATGCCAACATCAACGCCCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTg                                                                                      >  1:3585572/1‑99 (MQ=255)
               ccGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGt                                                                                    >  2:780131/1‑100 (MQ=255)
               ccGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGt                                                                                    <  1:433914/100‑1 (MQ=255)
               ccGACATCGTGAATGCCAACATCAACGCTCGGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGACGGAAGATACACTgg                                                                                     >  1:3489216/1‑99 (MQ=255)
                cGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGtt                                                                                   <  1:3794503/100‑1 (MQ=255)
                 gACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGACACTGGTTCGTCTGATGATCCAGAAGTTGGAAGATACACTGGTTg                                                                                  >  1:829511/1‑100 (MQ=255)
                  aCATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGa                                                                                 <  2:187452/100‑1 (MQ=255)
                   cATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGaa                                                                                <  1:2034700/100‑1 (MQ=255)
                   cATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGaa                                                                                >  2:230881/1‑100 (MQ=255)
                   cATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGaa                                                                                >  1:2957166/1‑100 (MQ=255)
                    aTCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAg                                                                               <  1:3424049/100‑1 (MQ=255)
                      cGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTa                                                                             <  2:3142394/100‑1 (MQ=255)
                      cGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTa                                                                             >  2:2573601/1‑100 (MQ=255)
                       gTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTAc                                                                            >  2:3668187/1‑100 (MQ=255)
                           aTGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTc                                                                        <  1:4154556/100‑1 (MQ=255)
                           aTGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTc                                                                        <  2:15849/100‑1 (MQ=255)
                             gCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTa                                                                      <  1:2210435/100‑1 (MQ=255)
                              ccAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTAc                                                                     >  1:952195/1‑100 (MQ=255)
                              ccAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTAc                                                                     >  2:3124320/1‑100 (MQ=255)
                               cAACATCAACGCTCTGTTAGAGAAAGCGGAAGCTCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACt                                                                    >  1:741505/1‑100 (MQ=255)
                               cAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACt                                                                    <  2:1212493/100‑1 (MQ=255)
                               cAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACt                                                                    <  1:1082533/100‑1 (MQ=255)
                                aaCATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACtt                                                                   >  1:365112/1‑100 (MQ=255)
                                  cATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCg                                                                 >  1:1020362/1‑100 (MQ=255)
                                   aTCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCgg                                                                >  1:2420826/1‑100 (MQ=255)
                                    tCAACGCTCTGTTAGAGAAAGCGGAAGATCCACCGAAACTGGTTCGTCTGATGCTCCAGGAGTTGGAAGATACGCTAGTTGAAGTACGTTCTACTTCgt                                                                <  1:2486324/99‑2 (MQ=255)
                                    tCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgc                                                               >  2:3801971/1‑100 (MQ=255)
                                     cAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcg                                                              <  1:383873/100‑1 (MQ=255)
                                     cAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcg                                                              >  2:3887060/1‑100 (MQ=255)
                                      aaCGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgc                                                             >  1:2291067/1‑100 (MQ=255)
                                       aCGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgcg                                                            >  2:3371395/1‑100 (MQ=255)
                                       aCGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgcg                                                            >  2:141582/1‑100 (MQ=255)
                                         gCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgcgcg                                                          <  1:2757200/100‑1 (MQ=255)
                                          ctctGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgcgcgc                                                         >  1:1631280/1‑100 (MQ=255)
                                          ctctGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGgcgcgcgc                                                         >  2:3961018/1‑100 (MQ=255)
                                           tctGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGcgcgcgcg                                                        <  2:220253/100‑1 (MQ=255)
                                            cgatctgagaAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGt                                                       <  1:2069310/94‑1 (MQ=255)
                                             tGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGtt                                                      >  2:330076/1‑100 (MQ=255)
                                              gTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTg                                                     >  1:3391055/1‑100 (MQ=255)
                                              gTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTg                                                     >  2:4101959/1‑100 (MQ=255)
                                              gTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTg                                                     >  2:3620347/1‑100 (MQ=255)
                                              gTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTg                                                     <  2:260685/100‑1 (MQ=255)
                                              gTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGAGGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGcgcgcgcg                                                        >  1:787024/1‑97 (MQ=255)
                                                tAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTgg                                                    <  1:3069731/99‑1 (MQ=255)
                                                 agagAAAGCGGAAGATCCACAGAAACTGGTTCGTCCGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCa                                                  <  1:502791/100‑1 (MQ=255)
                                                  gagaAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGtagatcg                                                 >  2:2069314/1‑94 (MQ=255)
                                                    gaACGCGGAATATCCACAGAAATTCGTTCGTCTGATGCTCCAGCAGATGGAAGATACACTGGTTTAAGTACGTTCTTTTTCGGCGCGCGCGTTCGCa                                                  <  1:2069093/97‑1 (MQ=255)
                                                      aaGCGGAAGATCCACAGAAACTGGTTCGTCTGATCATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCCTTGTCagaaaa                                             <  1:2244826/100‑1 (MQ=255)
                                                        gCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaaga                                           <  1:1065780/100‑1 (MQ=255)
                                                        gCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaaga                                           <  1:2693413/100‑1 (MQ=255)
                                                         cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaagaa                                          >  2:3917555/1‑100 (MQ=255)
                                                         cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaagaa                                          <  2:362646/100‑1 (MQ=255)
                                                          ggAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaagaaa                                         >  1:250864/1‑100 (MQ=255)
                                                          gaaaGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCagaaaagaaa                                         >  2:3965307/3‑100 (MQ=255)
                                                           gAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAAc                                        <  2:1701830/100‑1 (MQ=255)
                                                           gAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAAc                                        <  2:365114/100‑1 (MQ=255)
                                                            aaGATCCACAGAATCTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAAc                                        <  1:2032365/99‑1 (MQ=255)
                                                            aaGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACa                                       <  1:4019367/100‑1 (MQ=255)
                                                                tCCACAGAAGCTGGTTCGGCTGATGATCCAGGAGATGTAAGAGACACTTGTTGAAGTACGTTCTACTGCGGCGCGCGCGTTGGAAGAAAAGAAACAGCTg                                   >  1:2488270/1‑100 (MQ=255)
                                                                tCCACAGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTg                                   >  1:1468653/1‑100 (MQ=255)
                                                                tCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTg                                   <  1:1539739/100‑1 (MQ=255)
                                                                tCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTg                                   <  2:3410107/100‑1 (MQ=255)
                                                                  cacaGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGAc                                 >  1:3918342/1‑100 (MQ=255)
                                                                  cacaGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGAc                                 <  1:2266197/100‑1 (MQ=255)
                                                                   acaGAAACTGGTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGACAGAAAAGAAACAGCTGACt                                >  1:2757646/1‑100 (MQ=255)
                                                                     aGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTc                               <  1:3396334/99‑1 (MQ=255)
                                                                        aaCTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCg                           <  2:2563268/100‑1 (MQ=255)
                                                                             gTTCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTg                      <  2:3675453/100‑1 (MQ=255)
                                                                             gTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTg                      <  1:2376395/100‑1 (MQ=255)
                                                                              ttCGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGa                     >  1:1785054/1‑100 (MQ=255)
                                                                               tCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGaa                    <  1:421263/100‑1 (MQ=255)
                                                                                cGTCTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAAc                   <  1:3329215/100‑1 (MQ=255)
                                                                                cGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAAc                   <  1:1570919/100‑1 (MQ=255)
                                                                                 gTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACa                  <  2:1012187/100‑1 (MQ=255)
                                                                                   cTGATGATCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAg                <  1:1213301/100‑1 (MQ=255)
                                                                                    tgatgaTCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGc               >  1:1506254/1‑100 (MQ=255)
                                                                                    tgatgaTCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGc               >  2:2865670/1‑100 (MQ=255)
                                                                                     gatgatCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCg              >  2:85336/1‑100 (MQ=255)
                                                                                     gatgatCCAGGACTTGGAAGATACACTGGTTGAAGTACGTCCTACTTCGGCGCGCACGCTGGCAGCAAAGAAACCGCTGACTCGCCGTATTGACCAAGCg              >  1:1728052/1‑100 (MQ=255)
                                                                                      atgatCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGt             >  1:3743565/1‑100 (MQ=255)
                                                                                        gatCCAGGAGTTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCg           >  2:1917117/1‑100 (MQ=255)
                                                                                         atCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCgg          >  2:1504437/1‑100 (MQ=255)
                                                                                         atCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCgg          >  2:2635097/1‑100 (MQ=255)
                                                                                         atCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCgg          <  1:2717168/100‑1 (MQ=255)
                                                                                            cAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGgcgc       >  2:2925189/1‑100 (MQ=255)
                                                                                               gagaTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTg    <  2:792161/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
TTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAG  >  NC_000913/1368086‑1368282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: