BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,401,844	A→G	100%	I471T (ATC→ACC)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,844	0	A	G	100.0%	449.0 / ‑6.7	128	I471T (ATC→ACC)	abgB	p‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (65/62); minor base T (1/0); total (66/62)

Only 100 of 129 total aligned reads displayed.

GACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTG  >  NC_000913/1401748‑1401940

gacgacGGTATGGATGGCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAg                                                                                               <  1:1046332/100‑1 (MQ=255)

gacgacGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAg                                                                                               <  1:1961783/100‑1 (MQ=255)

   gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                            <  2:3954608/100‑1 (MQ=255)

   gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                            <  2:530375/100‑1 (MQ=255)

   gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTGGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                            >  2:3235004/1‑100 (MQ=255)

    acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                            >  1:798375/1‑99 (MQ=255)

    acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCa                                                                                           >  2:4243376/1‑100 (MQ=255)

    acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCa                                                                                           >  2:2859505/1‑100 (MQ=255)

        tATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGa                                                                                       <  2:689434/100‑1 (MQ=255)

         aTGTATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGAt                                                                                      <  1:3101219/100‑1 (MQ=255)

         aTGGATGACATACTCATGGGCATTCCTCGGTTGCTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGAt                                                                                      >  1:2707639/1‑100 (MQ=255)

          tGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATa                                                                                     >  2:3937477/1‑100 (MQ=255)

           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                    <  1:1432770/100‑1 (MQ=255)

           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                    >  2:2116566/1‑100 (MQ=255)

           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                    <  2:4247280/100‑1 (MQ=255)

           ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                    >  2:1028176/1‑100 (MQ=255)

            gATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACg                                                                                   <  2:1736749/100‑1 (MQ=255)

             aTGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACgg                                                                                  >  2:1771056/1‑100 (MQ=255)

                aCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTATGGCAGTGATACGGTTg                                                                               >  1:2494405/1‑100 (MQ=255)

                 cATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGc                                                                              >  1:694981/1‑100 (MQ=255)

                  aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCg                                                                             <  1:3031460/100‑1 (MQ=255)

                  aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCg                                                                             <  2:3840173/100‑1 (MQ=255)

                   tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                            >  1:325485/1‑100 (MQ=255)

                   tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                            >  1:3287678/1‑100 (MQ=255)

                   tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                            >  2:3102109/1‑100 (MQ=255)

                    aCTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtg                                                                           >  1:1525147/1‑100 (MQ=255)

                      tCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTc                                                                         <  1:2972969/100‑1 (MQ=255)

                       cATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTcc                                                                        >  2:1205300/1‑100 (MQ=255)

                        aTGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCg                                                                       <  2:908854/100‑1 (MQ=255)

                        aTGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCg                                                                       <  2:3705657/100‑1 (MQ=255)

                         tGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGt                                                                      >  1:3075850/1‑100 (MQ=255)

                           ggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGtt                                                                     <  2:95198/99‑1 (MQ=255)

                             cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                  <  2:3583970/100‑1 (MQ=255)

                             cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                  >  2:487160/1‑100 (MQ=255)

                              aTTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                  <  2:3470447/99‑1 (MQ=255)

                                tCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGc                                                               >  1:2576038/1‑100 (MQ=255)

                                tCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGc                                                               <  1:859722/100‑1 (MQ=255)

                                 ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGTTTATTTCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                              >  1:838971/1‑100 (MQ=255)

                                 ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                              <  1:1221674/100‑1 (MQ=255)

                                  cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                             >  2:4208447/1‑100 (MQ=255)

                                   tCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGa                                                            <  2:104489/100‑1 (MQ=255)

                                   tCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGa                                                            <  2:1209389/100‑1 (MQ=255)

                                    cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt                                                           >  1:345121/1‑100 (MQ=255)

                                      gttgttGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGc                                                         <  1:1697570/100‑1 (MQ=255)

                                        tgttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctc                                                       >  2:2273275/1‑100 (MQ=255)

                                        tgttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGct                                                        <  1:1094914/99‑1 (MQ=255)

                                         gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct                                                      >  2:2359824/1‑100 (MQ=255)

                                         gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct                                                      >  2:1875616/1‑100 (MQ=255)

                                           tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttg                                                    >  1:1274571/1‑100 (MQ=255)

                                           tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttg                                                    <  2:1274575/100‑1 (MQ=255)

                                           tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttg                                                    >  1:211727/1‑100 (MQ=255)

                                            gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTCATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgt                                                   <  1:2273269/100‑1 (MQ=255)

                                             tgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgtt                                                  <  1:1177846/100‑1 (MQ=255)

                                             tgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgtt                                                  >  2:2236652/1‑100 (MQ=255)

                                              gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTAATGCTCttgttg                                                 >  2:3169086/1‑100 (MQ=255)

                                               tttGCGTTGTTGTTTTTATTTTAAAGGGGACGGTGTCACGTTTTTCGTGGTAGGGCAGTGATACGGTTGCATGTCCGTTACTTGCTGATGCTCTTGTTgg                                                <  1:4101939/100‑1 (MQ=255)

                                               tttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTgg                                                <  2:2671352/100‑1 (MQ=255)

                                                 tGCGTTGTTGTTGTTATTTTAAAGGTGACTGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCa                                              <  2:2926806/100‑1 (MQ=255)

                                                 tGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCa                                              <  1:1955109/100‑1 (MQ=255)

                                                   cggtgtcgttgttATTTTAAAGGGGAAGGAGTCACGTTTGTCGGGGTTGGTCAGTTATACCGTTGCGTGTCCCTTGCTCGCTGATGCTCgtgttg                                                 >  1:2993556/4‑95 (MQ=255)

                                                   cGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCAtt                                            >  2:1825694/1‑100 (MQ=255)

                                                     ttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCt                                          <  2:1600148/100‑1 (MQ=255)

                                                      tgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCtt                                         <  2:2008476/100‑1 (MQ=255)

                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       <  2:2058098/100‑1 (MQ=255)

                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       <  2:3926022/100‑1 (MQ=255)

                                                        ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       >  2:2702439/1‑100 (MQ=255)

                                                          gttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                     >  2:2802740/1‑100 (MQ=255)

                                                           ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt                                    >  1:2935857/1‑100 (MQ=255)

                                                           ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt                                    >  2:1321302/1‑100 (MQ=255)

                                                            tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   >  1:94629/1‑100 (MQ=255)

                                                            tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   >  2:3395571/1‑100 (MQ=255)

                                                              ttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGc                                 >  1:526078/1‑100 (MQ=255)

                                                               tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                                >  2:3836378/1‑100 (MQ=255)

                                                               tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                                >  1:2879278/1‑100 (MQ=255)

                                                                 ttttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCt                              >  2:3947691/1‑100 (MQ=255)

                                                                  tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             <  2:694984/100‑1 (MQ=255)

                                                                  tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTATTGGCATACTTGCAATAGCCCTg                             >  2:4202949/1‑100 (MQ=255)

                                                                   ttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGa                            >  2:1475771/1‑100 (MQ=255)

                                                                   ttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGa                            >  2:2577615/1‑100 (MQ=255)

                                                                       aGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt                          <  1:1115967/98‑1 (MQ=255)

                                                                       aGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCa                        >  1:161184/1‑100 (MQ=255)

                                                                           gACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGa                    >  2:267218/1‑100 (MQ=255)

                                                                             cGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAag                  >  2:638842/1‑100 (MQ=255)

                                                                              ggTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                 <  2:3576854/100‑1 (MQ=255)

                                                                               gtgtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTATTGCAATAGCCCTGAATCAAGGAagag                >  2:515944/1‑100 (MQ=255)

                                                                                 gtCACGGTTTTCGGGGTAGTGGAGGGATACGTGTGCGTGTCCGTTACTTGCTGATGCTCTGGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt              <  2:2487717/100‑1 (MQ=255)

                                                                                   cccGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg            >  2:2894252/3‑100 (MQ=255)

                                                                                   cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGCTGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATACAGGCAGAGATTg            >  1:2646828/1‑100 (MQ=255)

                                                                                   cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg            >  2:1453805/1‑100 (MQ=255)

                                                                                     ngTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          >  2:2208527/2‑100 (MQ=255)

                                                                                     cGTTTTTCGGGGTATGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          >  1:3257188/1‑100 (MQ=255)

                                                                                     cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          >  1:3257037/1‑100 (MQ=255)

                                                                                     cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          <  1:808102/100‑1 (MQ=255)

                                                                                      gTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACt         <  1:2300416/100‑1 (MQ=255)

                                                                                      gTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACt         <  1:1076182/100‑1 (MQ=255)

                                                                                         tttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgtg      <  2:812499/100‑1 (MQ=255)

                                                                                          ttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg     <  2:2049745/100‑1 (MQ=255)

                                                                                           tCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGt    <  1:370402/100‑1 (MQ=255)

                                                                                            cGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt   <  1:356053/100‑1 (MQ=255)

                                                                                             ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtg  >  1:1454400/1‑100 (MQ=255)

GACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTG  >  NC_000913/1401748‑1401940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`