Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 235,082 A→G 47.0% K89K (AAA→AAG yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913235,0820AG47.0% 8.3 / 219.7 160K89K (AAA→AAGyafSputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (39/46);  new base G (29/46);  total (68/92)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

Only 100 of 164 total aligned reads displayed.

TGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGATGGCTG  >  NC_000913/234984‑235180
                                                                                                  |                                                                                                  
 gCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATc                                                                                                  >  1:1804790/1‑100 (MQ=255)
    aaGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGt                                                                                               <  1:4032589/100‑1 (MQ=255)
      gCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGtt                                                                                              <  1:1331815/99‑1 (MQ=255)
      gCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTg                                                                                             <  2:2335544/100‑1 (MQ=255)
      gCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTg                                                                                             <  2:2336208/100‑1 (MQ=255)
        gTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGAt                                                                                           <  1:3019520/100‑1 (MQ=255)
          gcgcGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGt                                                                                         >  1:1277116/1‑100 (MQ=255)
          gcgcGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGt                                                                                         >  2:306926/1‑100 (MQ=255)
           cgcgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGtt                                                                                        <  2:3784846/100‑1 (MQ=255)
           cgcgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGtt                                                                                        <  2:911535/100‑1 (MQ=255)
             cgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTg                                                                                      <  1:3410773/100‑1 (MQ=255)
             cgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTg                                                                                      <  2:695044/100‑1 (MQ=255)
             cgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTg                                                                                      <  2:2842737/100‑1 (MQ=255)
             cgGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTg                                                                                      >  2:2657591/1‑100 (MQ=255)
                tttCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCt                                                                                   <  1:2795144/100‑1 (MQ=255)
                tttCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCt                                                                                   <  2:3366798/100‑1 (MQ=255)
                 ttCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCACGTCCAGGTACAGGCGAATCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTa                                                                                  >  1:1999753/1‑100 (MQ=255)
                  tctcATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTAc                                                                                 <  1:426788/100‑1 (MQ=255)
                    tcatcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGCTAAATCCGTTGATGTTTGTCTACTg                                                                               >  1:535051/1‑100 (MQ=255)
                    tcatcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTg                                                                               <  2:3038131/100‑1 (MQ=255)
                    tcatcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTg                                                                               >  2:1711636/1‑100 (MQ=255)
                     catcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTgg                                                                              >  2:14654/1‑100 (MQ=255)
                      atcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGc                                                                             <  2:3125394/100‑1 (MQ=255)
                       tcaAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGca                                                                            <  2:2967872/100‑1 (MQ=255)
                        caAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGcac                                                                           >  2:3891306/1‑100 (MQ=255)
                         aaGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGcaca                                                                          <  1:3247026/100‑1 (MQ=255)
                          aGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCacat                                                                         >  1:2824847/1‑100 (MQ=255)
                             gAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCacataca                                                                      <  1:751923/100‑1 (MQ=255)
                                 gTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGc                                                                  <  1:1406339/100‑1 (MQ=255)
                                  tttCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGcc                                                                 >  2:3543363/1‑100 (MQ=255)
                                   ttCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCg                                                                <  1:168226/100‑1 (MQ=255)
                                      tGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCgtgg                                                             <  1:2354021/100‑1 (MQ=255)
                                       gcgcAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCgtggt                                                            <  2:2927414/100‑1 (MQ=255)
                                        cgcAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCgtggtg                                                           <  1:1019824/100‑1 (MQ=255)
                                         gcAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGc                                                          <  1:1143324/100‑1 (MQ=255)
                                         gcAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGc                                                          >  2:3858265/1‑100 (MQ=255)
                                         gcAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGc                                                          >  1:471732/1‑100 (MQ=255)
                                           aaGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCAc                                                        >  2:2158387/1‑100 (MQ=255)
                                            aGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCAcc                                                       <  1:3926450/100‑1 (MQ=255)
                                             ggAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCg                                                      >  2:2619443/1‑100 (MQ=255)
                                             ggAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCg                                                      >  2:3838304/1‑100 (MQ=255)
                                                 tGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCCCCGATc                                                   >  1:1918957/1‑99 (MQ=255)
                                                   cccGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGc                                                >  2:885445/1‑100 (MQ=255)
                                                     cGTCCAGGTACAGGCGGTCCCACTTCATCTTCCTTTGGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATTCATTGCCGTGGTGCACCGATCCg                                                 <  1:3308555/97‑1 (MQ=255)
                                                       tCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCg                                            <  2:1983482/100‑1 (MQ=255)
                                                         cAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGtt                                          >  1:3864371/1‑100 (MQ=255)
                                                             tACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTAtt                                      >  2:2788019/1‑100 (MQ=255)
                                                             tACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTAtt                                      <  1:2233968/100‑1 (MQ=255)
                                                              aCAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTg                                     <  2:1027908/100‑1 (MQ=255)
                                                               cAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGc                                    >  2:230580/1‑100 (MQ=255)
                                                                aGGCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCg                                   >  1:1655433/1‑100 (MQ=255)
                                                                aGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCg                                   <  2:4049273/100‑1 (MQ=255)
                                                                aGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCg                                   <  2:2993169/100‑1 (MQ=255)
                                                                 ggCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGt                                  >  1:910046/1‑100 (MQ=255)
                                                                 ggCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGt                                  >  1:1772083/1‑100 (MQ=255)
                                                                 ggCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGt                                  <  2:1358703/100‑1 (MQ=255)
                                                                 ggCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGt                                  >  2:1573335/1‑100 (MQ=255)
                                                                 ggCGGACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGt                                  <  1:372125/100‑1 (MQ=255)
                                                                   cGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGa                                >  2:598766/1‑100 (MQ=255)
                                                                    ggACCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGaa                               <  1:2564062/100‑1 (MQ=255)
                                                                     gACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAg                              >  2:2748839/1‑100 (MQ=255)
                                                                      aCCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAg                              <  1:3463712/99‑1 (MQ=255)
                                                                      aCCCACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGc                             <  1:4098648/100‑1 (MQ=255)
                                                                       cccACTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGcc                            >  2:1820527/1‑100 (MQ=255)
                                                                       cccACTTCATCTGCCTTTTGCCGATAAATCCGTTGATTTTTGTCTACTGTCACATACATTGCCGTGGTGCACCGCTCCGCATCGTTTATTGCGTGAAGc                             <  1:3935952/99‑1 (MQ=255)
                                                                         cACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGa                          >  2:2928526/1‑100 (MQ=255)
                                                                           cTTCATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATc                        <  1:1995091/100‑1 (MQ=255)
                                                                           cTTCATCTTCCTGTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGc                             <  1:2129739/95‑1 (MQ=255)
                                                                            ttCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCg                       <  2:1138884/100‑1 (MQ=255)
                                                                             tCATCTTCCTTTTGCCGATAAGTCCTTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCgg                      <  1:3699707/100‑1 (MQ=255)
                                                                              cATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCggg                     <  1:2921003/100‑1 (MQ=255)
                                                                              cATCTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCggg                     >  2:2857409/1‑100 (MQ=255)
                                                                              cATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCggg                     <  1:558801/100‑1 (MQ=255)
                                                                              cATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACAGACAGTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCggg                     <  1:397754/100‑1 (MQ=255)
                                                                               aTCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGt                    <  2:241237/100‑1 (MQ=255)
                                                                                 cTTCCTTTTGTCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTat                  >  1:314920/1‑100 (MQ=255)
                                                                                 cTTCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCggg                     >  1:3029055/1‑97 (MQ=255)
                                                                                 cTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTTAAGCCGATCGGGTat                  >  2:4206506/1‑100 (MQ=255)
                                                                                  ttCCTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTatt                 <  2:4080519/100‑1 (MQ=255)
                                                                                  ttCCTTTTGCCGATAAATCCGTTGATGTTTGTCTATTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATGGGGTatt                 <  2:2241549/100‑1 (MQ=255)
                                                                                    ccTTTTGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattga               <  1:2203232/100‑1 (MQ=255)
                                                                                    ccTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattga               <  1:1464735/100‑1 (MQ=255)
                                                                                     cTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattgat              >  1:1053255/1‑100 (MQ=255)
                                                                                     cTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattgat              >  2:2201591/1‑100 (MQ=255)
                                                                                      ttttGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATGGCCGTGGTGCACCGATCCGCAGCGTTTATTGCGTCAAGCCGATCGGGTattgat              <  1:363096/99‑1 (MQ=255)
                                                                                      ttttGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattgatt             <  1:1575792/100‑1 (MQ=255)
                                                                                      gatctccGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTattgatt             <  1:3071663/95‑1 (MQ=255)
                                                                                        ttGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTAttgattga           >  1:1160441/1‑100 (MQ=255)
                                                                                         tGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATtgattgat          <  1:128974/100‑1 (MQ=255)
                                                                                         tGCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATtgattgat          <  1:130350/100‑1 (MQ=255)
                                                                                          gCCGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATtgattgat          >  1:254223/1‑99 (MQ=255)
                                                                                           ccGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATtgatga        <  2:2586158/100‑1 (MQ=255)
                                                                                            cGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGCTCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTgatgat       >  1:3655340/1‑100 (MQ=255)
                                                                                            cGATAAGTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTgatgat       <  2:1874899/100‑1 (MQ=255)
                                                                                             gATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGatgatg      >  2:2628965/1‑100 (MQ=255)
                                                                                             gATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGatgatg      <  2:90386/100‑1 (MQ=255)
                                                                                               tAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAtggc    <  2:118524/100‑1 (MQ=255)
                                                                                               tAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAaggc    >  1:3661975/1‑100 (MQ=255)
                                                                                                 aaTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAtggctg  >  2:582843/1‑100 (MQ=255)
                                                                                                 aaTCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAtggctg  >  2:3107776/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
TGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGACCCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTGCCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGATGGCTG  >  NC_000913/234984‑235180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: