Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,460,438 T→C 46.5% L462S (TTG→TCG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,460,4380TC46.5% 21.8 / 212.7 157L462S (TTG→TCG) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base T (37/47);  new base C (39/34);  total (76/81)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC  >  NC_000913/1460341‑1460534
                                                                                                 |                                                                                                
cTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgc                                                                                                <  1:156025/100‑1 (MQ=255)
  tGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATtgctg                                                                                              >  2:772706/1‑100 (MQ=255)
   gCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgctgc                                                                                             <  2:2412355/100‑1 (MQ=255)
    ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcg                                                                                            >  1:1714614/1‑100 (MQ=255)
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTgcgc                                                                                           <  2:3994617/100‑1 (MQ=255)
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcgc                                                                                           >  1:4251294/1‑100 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                          >  2:3494124/1‑100 (MQ=255)
       ggggAGCGCAACTTGGCTGGCAGCGCATATTAAGGCTGCTTGAAAATCTACCGCATCACTATGGCGACGAACGCTATCGCCCATGTTCATcgct                                                                                               >  1:1079280/1‑94 (MQ=255)
       ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCACCTTGAAAATCTACAGCATCACTCTGGCGAAGAACGATATCGCCCATGTTCATTGCTGCGCCa                                                                                         >  1:3510214/1‑100 (MQ=255)
             cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgc                                                                                   <  2:1939466/100‑1 (MQ=255)
             cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgc                                                                                   >  2:4272238/1‑100 (MQ=255)
              gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgcg                                                                                  <  2:2744286/100‑1 (MQ=255)
              gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgcg                                                                                  <  2:1284427/100‑1 (MQ=255)
              gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgcg                                                                                  <  2:2550111/100‑1 (MQ=255)
              gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgcg                                                                                  <  1:1322108/100‑1 (MQ=255)
               cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgcgc                                                                                 >  1:229869/1‑100 (MQ=255)
               cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgcgc                                                                                 >  2:799263/1‑100 (MQ=255)
                aaCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCt                                                                                <  1:2944327/100‑1 (MQ=255)
                     ggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTgg                                                                           <  1:4241063/100‑1 (MQ=255)
                     ggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTgg                                                                           <  2:3025348/100‑1 (MQ=255)
                     ggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTgg                                                                           <  2:2041886/100‑1 (MQ=255)
                       ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGga                                                                          >  2:1755209/1‑99 (MQ=255)
                         ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgaga                                                                        <  1:3972799/99‑1 (MQ=255)
                         ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGagag                                                                       >  2:2915120/1‑100 (MQ=255)
                          gcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGc                                                                      >  1:447599/1‑100 (MQ=255)
                           cAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCg                                                                     <  1:2937389/100‑1 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCgg                                                                    <  2:1010998/100‑1 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCgg                                                                    <  1:3255467/100‑1 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCgg                                                                    >  1:662114/1‑100 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCgg                                                                    <  2:2179695/100‑1 (MQ=255)
                               gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTa                                                                 <  2:3313121/100‑1 (MQ=255)
                                aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTAt                                                                <  2:1030112/100‑1 (MQ=255)
                                 atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                               <  1:1112432/100‑1 (MQ=255)
                                 atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                               <  1:1190657/100‑1 (MQ=255)
                                  tgttgaGGCTCCTTGACAACCGACAGCATCACGATGGCGAGGAACGCGCTCGGCCATGTTCATTGCTGCGCCAACGGTCGCTTCTGGAGTTCGGTTa                                                                 <  1:3597735/92‑1 (MQ=255)
                                  tatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGa                                                              >  2:3552752/1‑100 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAATGGGCGCTTCTGGAGAGCGGTTATGAg                                                             <  2:2600455/100‑1 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             <  2:3371847/100‑1 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             <  2:230412/100‑1 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             <  2:1992369/100‑1 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             >  2:3651141/1‑100 (MQ=255)
                                     tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                           <  2:21700/100‑1 (MQ=255)
                                        ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATa                                                        >  1:2420473/1‑100 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTCTGAGTCATAAgg                                                     >  1:3365909/1‑100 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAgg                                                     <  1:487984/100‑1 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATCGCCAAGAACGCTATCGCTCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAgg                                                     <  1:1831443/100‑1 (MQ=255)
                                            ccTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGc                                                    >  1:581691/1‑100 (MQ=255)
                                             cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGcc                                                   <  1:1173097/100‑1 (MQ=255)
                                             cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGc                                                    >  1:237893/1‑99 (MQ=255)
                                             cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGcc                                                   <  2:662116/100‑1 (MQ=255)
                                              ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCt                                                  >  2:4245609/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGGGCGGTTATGAGTCATAAGGCCTg                                                 >  1:1831079/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 <  1:691538/100‑1 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 >  2:3796027/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTACGAGTCATAAGGcc                                                   >  1:3816844/1‑98 (MQ=255)
                                                 gaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGc                                               >  1:4084594/2‑100 (MQ=255)
                                                 aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGc                                               >  2:4164445/1‑100 (MQ=255)
                                                 aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTGCATCGCTGCGCCAACAGGAGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                                >  2:1841171/1‑99 (MQ=255)
                                                  aaaTCTACAGCATCACGATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGGCATAAGGCCTGGCa                                              <  1:3259634/100‑1 (MQ=255)
                                                   aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaa                                             <  1:4208661/100‑1 (MQ=255)
                                                       tACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATg                                         <  1:1640818/100‑1 (MQ=255)
                                                          aGCATCACTATGGCGAAGAACGCTATCGCTCATGTTCATTGCTGAACCAACGGGCGCATATGGAGAGCAGTAATAGGTCAAAAGACCTGGCAAAAAGcc                                       <  1:3327225/99‑1 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     >  1:91202/1‑100 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGccc                                      >  1:2055377/1‑99 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     >  1:144409/1‑100 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     >  1:3171675/1‑100 (MQ=255)
                                                            cATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCAt                                    <  1:2065219/100‑1 (MQ=255)
                                                             aTCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                   <  2:1683829/100‑1 (MQ=255)
                                                             aTCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                   <  2:206070/100‑1 (MQ=255)
                                                              tCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGc                                  <  2:957368/100‑1 (MQ=255)
                                                                aCTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCaa                                >  1:3417245/1‑100 (MQ=255)
                                                                   aTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                             <  1:2470888/100‑1 (MQ=255)
                                                                    tGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                            <  2:2954281/100‑1 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          <  2:63424/100‑1 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          >  2:3394954/1‑100 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          >  1:1299148/1‑100 (MQ=255)
                                                                       cGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATga                         >  2:3372296/1‑100 (MQ=255)
                                                                          agaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGaa                      >  1:1947050/1‑100 (MQ=255)
                                                                          agaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGaa                      >  1:916909/1‑100 (MQ=255)
                                                                           gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                     <  2:105860/100‑1 (MQ=255)
                                                                            aaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACg                    <  1:716825/100‑1 (MQ=255)
                                                                             aCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGa                   >  1:513348/1‑100 (MQ=255)
                                                                               gCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg                 >  2:151690/1‑100 (MQ=255)
                                                                                cTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCCGTTATGAGTCATAAGGACTGGCAAAATGCCCTTGCGAGAGATGAGAACggt                  >  1:2906212/1‑96 (MQ=255)
                                                                                  aTCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCt              <  2:833267/100‑1 (MQ=255)
                                                                                  aTCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCt              >  1:2427897/1‑100 (MQ=255)
                                                                                    cGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc            >  1:703005/1‑100 (MQ=255)
                                                                                    cGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTAATGAGTCATAAGGCCTGGCATAATGCCCATGCAATGTATGAGAAGGATGCCTg             >  1:736216/1‑99 (MQ=255)
                                                                                    cGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc            >  1:2754864/1‑100 (MQ=255)
                                                                                      cccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc          >  1:363811/1‑100 (MQ=255)
                                                                                      cccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc          <  2:3789658/100‑1 (MQ=255)
                                                                                       ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGcc         <  2:2830466/100‑1 (MQ=255)
                                                                                        cATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa        >  1:1626288/1‑100 (MQ=255)
                                                                                          tGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaaa      >  1:232935/1‑100 (MQ=255)
                                                                                           gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg     >  2:1408262/1‑100 (MQ=255)
                                                                                           gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg     <  2:3305749/100‑1 (MQ=255)
                                                                                           gTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg     >  2:2136849/1‑100 (MQ=255)
                                                                                            ttCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg     >  2:1334582/1‑99 (MQ=255)
                                                                                             tCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcg   >  2:3208796/1‑100 (MQ=255)
                                                                                              cATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcgc  >  1:2003164/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC  >  NC_000913/1460341‑1460534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: