Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,222 A→T 15.1% intergenic (+21/+33) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2220AT15.1% 373.1 / 47.7 144intergenic (+21/+33)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (56/66);  new base T (11/11);  total (67/77)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 152 total aligned reads displayed.

CTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGA  >  NC_000913/1482123‑1482321
                                                                                                   |                                                                                                   
cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGa                                                                                                     <  1:247012/100‑1 (MQ=255)
cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGa                                                                                                     <  2:1575599/100‑1 (MQ=255)
cTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGa                                                                                                     <  2:1575668/100‑1 (MQ=255)
 tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGaa                                                                                                    <  1:2392253/100‑1 (MQ=255)
 tataTTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGaa                                                                                                    <  1:2820919/100‑1 (MQ=255)
  atatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAat                                                                                                   <  2:4191322/100‑1 (MQ=255)
   tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAata                                                                                                  <  1:2102024/100‑1 (MQ=255)
    atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                 >  2:1887543/1‑100 (MQ=255)
    atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAACGAGGCTGAGAGTATGGCAAAACCGGTAAGCGGATTGATATGTGGACTGAata                                                                                                  >  1:2125894/1‑99 (MQ=255)
     ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                <  2:2760630/100‑1 (MQ=255)
      tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                               <  1:262417/100‑1 (MQ=255)
       cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                              >  2:3344760/1‑100 (MQ=255)
       cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                              >  1:669543/1‑100 (MQ=255)
          gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                           <  2:3449194/100‑1 (MQ=255)
          gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                           <  1:3525742/100‑1 (MQ=255)
           ccAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGCAAACAGGTAAGTGGATTGAGATGTGGACCGAATATCTACAGt                                                                                          <  2:2892922/100‑1 (MQ=255)
            cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                         >  2:2465227/1‑100 (MQ=255)
            cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                         <  2:1248554/100‑1 (MQ=255)
               aCGACCGCAGATTGTGCCGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTAGATTGAGATGTGGACTGTAGATATTCAGTCcac                                                                                      <  1:1159959/100‑1 (MQ=37)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                     >  1:114619/1‑100 (MQ=38)
                cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                     >  2:631762/1‑100 (MQ=255)
                 gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                    <  2:4234019/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                   <  2:4064442/100‑1 (MQ=38)
                  cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                   <  2:114619/100‑1 (MQ=38)
                     gCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctca                                                                                <  2:2996454/100‑4 (MQ=38)
                     gCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAg                                                                                <  2:3275434/100‑1 (MQ=255)
                       aGATTGTGATGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGACcaca                                                                                     <  1:3614553/93‑1 (MQ=255)
                           tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                          <  1:683209/100‑1 (MQ=255)
                            gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                         >  2:3148875/1‑100 (MQ=255)
                            gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                         >  1:1170757/1‑100 (MQ=255)
                            gtgCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                          >  2:3898269/1‑99 (MQ=255)
                               cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                      <  2:899012/100‑1 (MQ=255)
                                 gtcAGACGAGCAGAGAGTGATGCTGTGATTTTGGGAAAACAGGTAAGTGGATTGAGATGGGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                         <  1:2469426/93‑1 (MQ=255)
                                   cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGCACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                  >  1:32698/1‑100 (MQ=255)
                                     gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                <  1:3190744/100‑1 (MQ=255)
                                     gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                >  2:3734808/1‑100 (MQ=255)
                                     gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                <  2:1729052/100‑1 (MQ=255)
                                      aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                               <  1:3848098/100‑1 (MQ=255)
                                      aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                               <  2:1993382/100‑1 (MQ=255)
                                       cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                              >  2:3478555/1‑100 (MQ=255)
                                       cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                              <  2:304240/100‑1 (MQ=255)
                                        gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAg                                                             >  2:172177/1‑100 (MQ=255)
                                          gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGaa                                                           >  1:3132349/1‑100 (MQ=255)
                                             caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                        >  2:3224158/1‑100 (MQ=255)
                                             caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                        >  2:1511053/1‑100 (MQ=255)
                                               aaGCGATGCTGAGATTATGGAAAAACAGGTAAGTGGAGTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                      <  2:3388373/100‑1 (MQ=255)
                                                aGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATg                                                     <  1:1621742/100‑1 (MQ=255)
                                                 gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                    <  2:1195071/100‑1 (MQ=255)
                                                 gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                    <  1:125245/100‑1 (MQ=255)
                                                  cGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                   >  1:1079860/1‑100 (MQ=255)
                                                    aTGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGt                                                 <  2:403558/100‑1 (MQ=255)
                                                     tGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTc                                                <  2:2430247/100‑1 (MQ=255)
                                                      gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGGTATGCAGAAACAATGCTGTCg                                               >  1:2903835/1‑100 (MQ=255)
                                                       cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                              >  1:3561349/1‑100 (MQ=255)
                                                           gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGCCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                          <  2:415253/100‑1 (MQ=255)
                                                           gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                          <  1:4100860/100‑1 (MQ=255)
                                                              tATGGGAAACCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgc                                       <  2:796217/100‑1 (MQ=255)
                                                               aTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgct                                      >  1:2517054/1‑100 (MQ=255)
                                                                tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                     >  2:3237336/1‑100 (MQ=255)
                                                                tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                     >  2:1915227/1‑100 (MQ=255)
                                                                 gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                    >  2:1973363/1‑100 (MQ=255)
                                                                  ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                   >  2:3096293/1‑100 (MQ=255)
                                                                   gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                  <  2:3981737/100‑1 (MQ=255)
                                                                   gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                  <  2:819658/100‑1 (MQ=255)
                                                                      aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGt                               >  1:196234/1‑100 (MQ=255)
                                                                        cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCa                             >  1:1845187/1‑100 (MQ=255)
                                                                        cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCa                             <  2:3707900/100‑1 (MQ=255)
                                                                                 ggATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCt                    <  2:198418/100‑1 (MQ=255)
                                                                                  gATTGAGATGTGTACTGAATATCTACAGTCCACATCAAGAACGTGTCCGGTTATGCAGCAACAATGCTGTCGATGGATGCTTTTGCGTCAGACTGTGCtt                   >  1:1427027/1‑100 (MQ=255)
                                                                                  gATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCtt                   >  2:1098903/1‑100 (MQ=255)
                                                                                   gtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                  >  1:3828725/6‑100 (MQ=37)
                                                                                    ttGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTc                 <  1:889131/100‑1 (MQ=255)
                                                                                     tGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                <  1:2940231/100‑1 (MQ=255)
                                                                                      gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  2:1341286/1‑100 (MQ=255)
                                                                                      gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  2:2341576/1‑100 (MQ=255)
                                                                                       tgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTGTCGCt              >  1:4058946/2‑100 (MQ=38)
                                                                                       agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt              <  2:3012169/100‑1 (MQ=255)
                                                                                        gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  1:2297057/1‑100 (MQ=38)
                                                                                        gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  1:3474430/1‑100 (MQ=38)
                                                                                        gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             <  2:2919670/100‑1 (MQ=255)
                                                                                        gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  1:1326285/1‑100 (MQ=255)
                                                                                        gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  2:2459159/1‑100 (MQ=255)
                                                                                        gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             <  1:2912148/100‑1 (MQ=255)
                                                                                         aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc            >  2:1005106/1‑100 (MQ=255)
                                                                                          tgtgTACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           <  2:684668/100‑1 (MQ=255)
                                                                                          tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           >  1:753942/1‑100 (MQ=38)
                                                                                          tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           >  2:3205445/1‑100 (MQ=38)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           <  2:3949565/100‑1 (MQ=255)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           >  1:1382949/1‑100 (MQ=255)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           <  2:4715/100‑1 (MQ=255)
                                                                                          tgtgGACTGAATATCTACAGTCCACATCAAGAACGTGTCAGGTTATGCAGACACAATGCTGTCGATGCCTGCTTTTCCGTCAGACTGTGCCCTCGCTGcc           <  1:856880/100‑1 (MQ=255)
                                                                                           gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa          >  2:1858198/1‑100 (MQ=38)
                                                                                            tgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATCCAGAAACAATGCTGTCGCTGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAt         >  1:3333059/1‑100 (MQ=38)
                                                                                              gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt       >  2:3919379/1‑100 (MQ=255)
                                                                                              gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt       >  1:1605376/1‑100 (MQ=255)
                                                                                                cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc     <  1:3491217/100‑1 (MQ=255)
                                                                                                cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc     >  2:2078394/1‑100 (MQ=255)
                                                                                                 tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg    >  2:1071566/1‑100 (MQ=255)
                                                                                                  naaTATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg   >  1:3601917/2‑100 (MQ=255)
                                                                                                   aaTATCTACAGTCCACATCAAGTCCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGa  >  1:2474776/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGA  >  NC_000913/1482123‑1482321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: