Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,224 T→G 15.5% intergenic (+23/+31) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2240TG15.5% 363.5 / 58.3 142intergenic (+23/+31)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base T (56/64);  new base G (11/11);  total (67/75)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

Only 100 of 146 total aligned reads displayed.

ATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGA  >  NC_000913/1482125‑1482321
                                                                                                   |                                                                                                 
 tatTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAata                                                                                                  <  1:2102024/100‑1 (MQ=255)
  atTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAatat                                                                                                 >  2:1887543/1‑100 (MQ=255)
   ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                <  2:2760630/100‑1 (MQ=255)
    tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                               >  2:329801/1‑100 (MQ=255)
    tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                               >  2:3143164/1‑100 (MQ=255)
     cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                              >  1:669543/1‑100 (MQ=255)
     cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                              <  2:3391523/100‑1 (MQ=255)
      gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                              <  1:2470812/99‑1 (MQ=255)
      gAGCCAGACGCCAGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGGGGATTGAGATGTGGACTGAATATCTAc                                                                                             <  2:2365794/100‑1 (MQ=255)
        gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                           >  1:3420295/1‑100 (MQ=255)
          cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                         <  2:1248554/100‑1 (MQ=255)
          cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                         >  2:2459551/1‑100 (MQ=255)
          cAGACGACCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                         <  1:1156685/100‑1 (MQ=255)
            gACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                       <  2:1243241/100‑1 (MQ=255)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                     >  1:114619/1‑100 (MQ=38)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                     <  1:3958615/100‑1 (MQ=38)
              cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                     >  2:631762/1‑100 (MQ=255)
               gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                    <  2:4234019/100‑1 (MQ=38)
                cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                   <  2:114619/100‑1 (MQ=38)
                cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                   <  1:3207316/100‑1 (MQ=38)
                 ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                  <  2:4200250/100‑1 (MQ=255)
                   gCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAg                                                                                <  2:3275434/100‑1 (MQ=255)
                     aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                              <  2:3934841/100‑1 (MQ=255)
                     aGATTGTGATGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGACcaca                                                                                     <  1:3614553/93‑1 (MQ=255)
                      gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatc                                                                             <  2:3539567/100‑7 (MQ=25)
                         tgtgGTGACAGACGAGCACAAAGCGATGATGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                          <  1:3582907/100‑1 (MQ=255)
                         tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                          <  2:721047/100‑1 (MQ=255)
                          gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatccact                                                                         <  2:2822639/100‑11 (MQ=21)
                          gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                         >  1:1170757/1‑100 (MQ=255)
                            gCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTcc                                                                       >  1:4158435/1‑100 (MQ=255)
                             cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                      <  2:899012/100‑1 (MQ=255)
                             cTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                      >  2:385502/1‑100 (MQ=255)
                               gtcAGACGAGCAGAGAGTGATGCTGTGATTTTGGGAAAACAGGTAAGTGGATTGAGATGGGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                         <  1:2469426/93‑1 (MQ=255)
                               gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                    <  2:1066900/100‑1 (MQ=255)
                                   gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                <  2:1729052/100‑1 (MQ=255)
                                   gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                >  2:3734808/1‑100 (MQ=255)
                                   gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                <  1:3190744/100‑1 (MQ=255)
                                    aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                               <  1:2830828/100‑1 (MQ=255)
                                    aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                               <  2:1993382/100‑1 (MQ=255)
                                    aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                               <  1:3848098/100‑1 (MQ=255)
                                     cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                              >  1:868292/1‑100 (MQ=255)
                                     cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                              >  2:3478555/1‑100 (MQ=255)
                                      gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAg                                                             <  1:135484/100‑1 (MQ=255)
                                      gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAg                                                             >  2:172177/1‑100 (MQ=255)
                                        gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGaa                                                           >  1:3132349/1‑100 (MQ=255)
                                           caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                        >  2:3224158/1‑100 (MQ=255)
                                           caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                        >  2:1511053/1‑100 (MQ=255)
                                             aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                      >  1:3665486/1‑100 (MQ=255)
                                             aaGCGATGCTGAGATTATGGAAAAACAGGTAAGTGGAGTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                      <  2:3388373/100‑1 (MQ=255)
                                              aGCGATGCTGATATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                      <  1:2004595/99‑1 (MQ=255)
                                              aGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATg                                                     <  1:1621742/100‑1 (MQ=255)
                                               gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                    >  2:596800/1‑100 (MQ=255)
                                                cGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                   >  1:1079860/1‑100 (MQ=255)
                                                  aTGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGt                                                 <  2:403558/100‑1 (MQ=255)
                                                   tGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                    <  2:4091702/96‑1 (MQ=255)
                                                    gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                               >  1:3502360/1‑100 (MQ=255)
                                                    gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGGTATGCAGAAACAATGCTGTCg                                               >  1:2903835/1‑100 (MQ=255)
                                                     cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                              >  1:3561349/1‑100 (MQ=255)
                                                        agaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATgg                                           <  1:2266022/100‑1 (MQ=255)
                                                         gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGCCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                          <  2:415253/100‑1 (MQ=255)
                                                         gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                          <  1:4100860/100‑1 (MQ=255)
                                                            tATGGGAAACCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgc                                       <  2:796217/100‑1 (MQ=255)
                                                             aTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgct                                      >  1:2517054/1‑100 (MQ=255)
                                                              tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                     >  2:1915227/1‑100 (MQ=255)
                                                               gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                    >  2:1973363/1‑100 (MQ=255)
                                                                ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                   >  2:1538913/1‑100 (MQ=255)
                                                                ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                   >  2:3096293/1‑100 (MQ=255)
                                                                 gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                  <  2:3981737/100‑1 (MQ=255)
                                                                 gAAAACAGGTAAGTGGATTGAGATGTCGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTTTCGATGGCTGCTTTTg                                  <  1:2924899/100‑1 (MQ=255)
                                                                    aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCAGATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                 <  1:2580288/98‑1 (MQ=255)
                                                                    aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGt                               >  1:196234/1‑100 (MQ=255)
                                                                     aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                              >  2:2957606/1‑100 (MQ=255)
                                                                      cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCa                             >  2:3150451/1‑100 (MQ=255)
                                                                        ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAg                            >  1:1216203/1‑99 (MQ=255)
                                                                               ggATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCt                    <  2:198418/100‑1 (MQ=255)
                                                                                gATTGAGATGTGTACTGAATATCTACAGTCCACATCAAGAACGTGTCCGGTTATGCAGCAACAATGCTGTCGATGGATGCTTTTGCGTCAGACTGTGCtt                   >  1:1427027/1‑100 (MQ=255)
                                                                                  ttGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTc                 <  1:889131/100‑1 (MQ=255)
                                                                                   tGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                >  2:303813/1‑100 (MQ=255)
                                                                                   tGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                <  1:2940231/100‑1 (MQ=255)
                                                                                    gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  2:2341576/1‑100 (MQ=255)
                                                                                    gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  2:1341286/1‑100 (MQ=255)
                                                                                    gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  1:1686480/1‑100 (MQ=255)
                                                                                    gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc               >  1:684665/1‑100 (MQ=255)
                                                                                     tgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGAATCTGCTATCACt              >  2:3997971/2‑100 (MQ=25)
                                                                                     agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt              <  2:3012169/100‑1 (MQ=255)
                                                                                     agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt              <  2:835732/100‑1 (MQ=255)
                                                                                      gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  1:2297057/1‑100 (MQ=38)
                                                                                      gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  1:1326285/1‑100 (MQ=255)
                                                                                      gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             <  2:2919670/100‑1 (MQ=255)
                                                                                      gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg             >  2:2459159/1‑100 (MQ=255)
                                                                                        tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           >  1:753942/1‑100 (MQ=38)
                                                                                        tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc           >  2:3205445/1‑100 (MQ=38)
                                                                                         gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa          >  2:1858198/1‑100 (MQ=38)
                                                                                          tgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATCCAGAAACAATGCTGTCGCTGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAt         >  1:3333059/1‑100 (MQ=38)
                                                                                            gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt       >  2:3919379/1‑100 (MQ=255)
                                                                                              cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc     <  1:3491217/100‑1 (MQ=255)
                                                                                              cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc     <  1:1565631/100‑1 (MQ=255)
                                                                                              cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc     >  2:2078394/1‑100 (MQ=255)
                                                                                               tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg    >  2:1071566/1‑100 (MQ=255)
                                                                                               tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg    <  2:1121811/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
ATATTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGA  >  NC_000913/1482125‑1482321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: