Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,227 C→A 15.4% intergenic (+26/+28) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2270CA15.4% 393.3 / 53.1 143intergenic (+26/+28)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (57/64);  new base A (11/11);  total (68/75)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 146 total aligned reads displayed.

TTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTA  >  NC_000913/1482128‑1482326
                                                                                                   |                                                                                                   
ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                     <  2:2760630/100‑1 (MQ=255)
ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                     <  2:1980633/100‑1 (MQ=255)
 tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                                    >  2:3143164/1‑100 (MQ=255)
 tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                                    <  1:262417/100‑1 (MQ=255)
 tCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCt                                                                                                    >  2:329801/1‑100 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                   >  1:669543/1‑100 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                   <  2:3391523/100‑1 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                   >  1:3131061/1‑100 (MQ=255)
   gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                   <  1:2470812/99‑1 (MQ=255)
   gAGCCAGACGCCAGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGGGGATTGAGATGTGGACTGAATATCTAc                                                                                                  <  2:2365794/100‑1 (MQ=255)
     gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                                <  2:3449194/100‑1 (MQ=255)
     gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                                >  1:3420295/1‑100 (MQ=255)
     gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                                <  1:3525742/100‑1 (MQ=255)
       cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                              >  2:2459551/1‑100 (MQ=255)
       cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                              <  2:1248554/100‑1 (MQ=255)
       cAGACGACCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                              <  1:1156685/100‑1 (MQ=255)
           cGCCCGCAGATTGTGCTTACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                          <  1:2392964/100‑1 (MQ=38)
           cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                          <  1:3958615/100‑1 (MQ=38)
           cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                          >  1:114619/1‑100 (MQ=38)
           cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCcaca                                                                                          >  2:631762/1‑100 (MQ=255)
            gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                         <  2:2059407/100‑1 (MQ=38)
            gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                         <  2:4234019/100‑1 (MQ=38)
             cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                        <  2:114619/100‑1 (MQ=38)
             cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                        <  2:4064442/100‑1 (MQ=38)
             cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                        <  1:3207316/100‑1 (MQ=38)
              ccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                       <  2:4200250/100‑1 (MQ=255)
                gCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAg                                                                                     <  2:3275434/100‑1 (MQ=255)
                      tgtgGTGACAGACGAGCACAAAGCGATGATGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                               <  1:3582907/100‑1 (MQ=255)
                      tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                               <  2:721047/100‑1 (MQ=255)
                      tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                               <  1:683209/100‑1 (MQ=255)
                       gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatccact                                                                              <  2:2822639/100‑11 (MQ=21)
                       gtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtgt                                                                              >  1:1170757/1‑100 (MQ=255)
                       gtgCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                               >  2:3898269/1‑99 (MQ=255)
                         gCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTcc                                                                            >  1:4158435/1‑100 (MQ=255)
                          cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                           <  2:1271449/100‑1 (MQ=255)
                          cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                           <  2:899012/100‑1 (MQ=255)
                              cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGCACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                       >  1:32698/1‑100 (MQ=255)
                                gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                     <  2:1729052/100‑1 (MQ=255)
                                gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                     >  2:3734808/1‑100 (MQ=255)
                                gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                     <  1:3190744/100‑1 (MQ=255)
                                 aCGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGc                                                                    <  2:1993382/100‑1 (MQ=255)
                                  cGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCa                                                                   >  2:3478555/1‑100 (MQ=255)
                                   gAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAg                                                                  <  1:135484/100‑1 (MQ=255)
                                        caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                             >  2:3224158/1‑100 (MQ=255)
                                        caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                             >  2:1511053/1‑100 (MQ=255)
                                          aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                           >  1:3665486/1‑100 (MQ=255)
                                          aaGCGATGCTGAGATTATGGAAAAACAGGTAAGTGGAGTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                           <  2:3388373/100‑1 (MQ=255)
                                           aGCGATGCTGATATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                           <  1:2004595/99‑1 (MQ=255)
                                           aGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATg                                                          <  2:1895127/100‑1 (MQ=255)
                                           aGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATg                                                          <  1:1621742/100‑1 (MQ=255)
                                            gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                         >  2:596800/1‑100 (MQ=255)
                                            gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                         <  2:1195071/100‑1 (MQ=255)
                                             cGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCt                                                        >  1:1079860/1‑100 (MQ=255)
                                                tGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                         <  2:4091702/96‑1 (MQ=255)
                                                 gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                    >  1:3502360/1‑100 (MQ=255)
                                                 gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGGTATGCAGAAACAATGCTGTCg                                                    >  1:2903835/1‑100 (MQ=255)
                                                         tATGGGAAACCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctgc                                            <  2:796217/100‑1 (MQ=255)
                                                            gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                         >  2:1973363/1‑100 (MQ=255)
                                                             ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                        >  2:1538913/1‑100 (MQ=255)
                                                             ggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtttt                                        >  2:3096293/1‑100 (MQ=255)
                                                              gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                       <  2:3981737/100‑1 (MQ=255)
                                                                 aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCAGATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGc                                      <  1:2580288/98‑1 (MQ=255)
                                                                 aaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGt                                    >  1:196234/1‑100 (MQ=255)
                                                                  aCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTc                                   >  2:2957606/1‑100 (MQ=255)
                                                                   cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCa                                  >  2:3150451/1‑100 (MQ=255)
                                                                   cAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCa                                  <  2:3707900/100‑1 (MQ=255)
                                                                     ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAg                                 >  1:1216203/1‑99 (MQ=255)
                                                                             gATTGAGATGTGTACTGAATATCTACAGTCCACATCAAGAACGTGTCCGGTTATGCAGCAACAATGCTGTCGATGGATGCTTTTGCGTCAGACTGTGCtt                        >  1:1427027/1‑100 (MQ=255)
                                                                             gATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCtt                        >  2:1098903/1‑100 (MQ=255)
                                                                              gtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                       >  1:3828725/6‑100 (MQ=37)
                                                                               ttGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTc                      <  1:889131/100‑1 (MQ=255)
                                                                                tGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                     >  2:303813/1‑100 (MQ=255)
                                                                                tGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCg                     <  1:2940231/100‑1 (MQ=255)
                                                                                 gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc                    >  1:1686480/1‑100 (MQ=255)
                                                                                 gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc                    >  2:2341576/1‑100 (MQ=255)
                                                                                  tgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTGTCGCt                   >  1:4058946/2‑100 (MQ=38)
                                                                                  tgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGAATCTGCTATCACt                   >  2:3997971/2‑100 (MQ=25)
                                                                                  agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt                   <  2:3012169/100‑1 (MQ=255)
                                                                                  agaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCt                   <  2:835732/100‑1 (MQ=255)
                                                                                   gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  >  1:3474430/1‑100 (MQ=38)
                                                                                   gaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  >  1:2297057/1‑100 (MQ=38)
                                                                                   gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  <  1:2912148/100‑1 (MQ=255)
                                                                                   gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  >  2:2459159/1‑100 (MQ=255)
                                                                                   gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  >  1:1326285/1‑100 (MQ=255)
                                                                                   gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                  <  2:2919670/100‑1 (MQ=255)
                                                                                    aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                 >  2:1005106/1‑100 (MQ=255)
                                                                                     tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc                >  2:3205445/1‑100 (MQ=38)
                                                                                     tgtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc                >  1:753942/1‑100 (MQ=38)
                                                                                     tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc                <  2:3949565/100‑1 (MQ=255)
                                                                                     tgtgGACTGAATATCTACAGTCCACATCAAGAACGTGTCAGGTTATGCAGACACAATGCTGTCGATGCCTGCTTTTCCGTCAGACTGTGCCCTCGCTGcc                <  1:856880/100‑1 (MQ=255)
                                                                                      gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa               >  2:1858198/1‑100 (MQ=38)
                                                                                         gACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAttt            >  2:3919379/1‑100 (MQ=255)
                                                                                           cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc          <  1:1565631/100‑1 (MQ=255)
                                                                                           cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc          <  2:1646469/100‑1 (MQ=255)
                                                                                            tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg         >  2:1071566/1‑100 (MQ=255)
                                                                                             naaTATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg        >  1:3601917/2‑100 (MQ=255)
                                                                                              aaTATCTACAGTCCACATCAAGTCCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGa       >  1:2474776/1‑100 (MQ=255)
                                                                                                 atCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg    <  1:1850149/100‑1 (MQ=255)
                                                                                                  tCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGt   <  2:1792771/100‑1 (MQ=255)
                                                                                                   cTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTa  >  2:2328071/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTA  >  NC_000913/1482128‑1482326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: