Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,515,267 A→T 34.8% D169V (GAT→GTT)  ydcV → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,515,2670AT34.8% 182.1 / 154.9 167D169V (GAT→GTT) ydcVputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (51/56);  new base T (37/20);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 4.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTT  >  NC_000913/1515168‑1515366
                                                                                                   |                                                                                                   
tCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGa                                                                                                     <  2:4042108/100‑1 (MQ=255)
     gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCtt                                                                                                 >  1:3929468/1‑99 (MQ=255)
       cggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTggg                                                                                              <  2:2355704/100‑1 (MQ=255)
       cggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTggg                                                                                              <  1:2267815/100‑1 (MQ=255)
       cggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTggg                                                                                              <  1:152532/100‑1 (MQ=255)
        ggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTgggg                                                                                             <  1:4053114/100‑1 (MQ=255)
         gtcATGCGACTTTTTTTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGGTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGACAATGGATCTTGGGGc                                                                                            >  1:2489055/1‑100 (MQ=255)
         gtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGc                                                                                            <  1:3415640/100‑1 (MQ=255)
             tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAt                                                                                        <  1:3099816/100‑1 (MQ=255)
              gCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtg                                                                                       >  1:1145353/1‑100 (MQ=255)
              gCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtg                                                                                       >  1:1037701/1‑100 (MQ=255)
               cGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtgg                                                                                      >  2:367050/1‑100 (MQ=255)
                gACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtg                                                                                       >  1:1712467/1‑98 (MQ=255)
                   tttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggctgg                                                                                  >  2:949451/1‑100 (MQ=255)
                   tttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggctgg                                                                                  >  2:3096690/1‑100 (MQ=255)
                    ttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtggctggc                                                                                 <  1:2206269/100‑1 (MQ=255)
                     tgtgtgTAGTTGTGGTGTTGAACAATGTCATCGCCCGTTTTCGCCGGACCTCCTGGAGTCTGGTTGAGGCGTCAATGGGTCTTGGGGCCAAtggctg                                                                                   <  1:1471361/95‑1 (MQ=255)
                        gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAAc                                                                             >  2:1435244/1‑100 (MQ=255)
                        gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAAc                                                                             >  1:851886/1‑100 (MQ=255)
                         tgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAAcc                                                                            >  1:3580440/1‑100 (MQ=255)
                            aGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTc                                                                         >  2:3975922/1‑100 (MQ=255)
                             gTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTcc                                                                        <  2:3110169/100‑1 (MQ=255)
                               tgtgGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGc                                                                      <  1:1246219/100‑1 (MQ=255)
                               tgtgGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGc                                                                      >  2:4000654/1‑100 (MQ=255)
                                gtggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCGGGTTGAGGCGTCAATGGATCTTGGGCCCAATGGCTGGCAAACCTTCCGCt                                                                     >  2:2816476/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                   >  2:3113000/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                   >  1:789786/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                   >  2:4150658/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                   >  1:3025918/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                   >  2:659140/1‑100 (MQ=255)
                                   gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACg                                                                  >  1:3045663/1‑100 (MQ=255)
                                    tgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACg                                                                  <  1:3150226/99‑1 (MQ=255)
                                    tgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGt                                                                 <  2:1145356/100‑1 (MQ=255)
                                     gtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTa                                                                >  2:3742704/1‑100 (MQ=255)
                                     gtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTa                                                                >  2:4222979/1‑100 (MQ=255)
                                     gtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTa                                                                >  2:3021022/1‑100 (MQ=255)
                                       ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTTCGCTACGTAgt                                                              >  2:3722167/1‑100 (MQ=255)
                                       ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgt                                                              >  2:4009476/1‑100 (MQ=255)
                                       ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgt                                                              <  1:2603702/100‑1 (MQ=255)
                                       ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgt                                                              >  1:106139/1‑100 (MQ=255)
                                          aCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGtt                                                           <  2:326413/100‑1 (MQ=255)
                                             aTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGcc                                                        >  2:3867621/1‑100 (MQ=255)
                                                 cATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAt                                                    >  1:3295054/1‑100 (MQ=255)
                                                 cATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAt                                                    >  2:1814697/1‑100 (MQ=255)
                                                   tCGCCCGTTTTCGCCGCACCTCCTGTAGGCTGGTTGAGGCGTCAATGGATCTTGGCGCCATTGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAtc                                                   <  1:4268874/99‑1 (MQ=255)
                                                    cGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAtctc                                                 >  1:2822184/1‑100 (MQ=255)
                                                    cGCCCGCTTTCGCCGCCCGTCCTGGAGTCTTGTTGAGGCGTCGATGGATCTTGGGGCCAATGGCTGGGAAACCTTCCGCTACGTAGTGTTGCCGAAtct                                                  <  1:4139760/99‑1 (MQ=255)
                                                     gCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCa                                                >  1:2698412/1‑100 (MQ=255)
                                                     gCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCa                                                >  1:651003/1‑100 (MQ=255)
                                                     gCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCa                                                >  1:3750540/1‑100 (MQ=255)
                                                      cccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAg                                               >  2:613810/1‑100 (MQ=255)
                                                       ccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGt                                              >  1:1781190/1‑100 (MQ=255)
                                                        cGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGtt                                             <  2:3445253/100‑1 (MQ=255)
                                                           tttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCgg                                          <  2:1828612/100‑1 (MQ=255)
                                                            ttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGc                                         <  1:1939634/100‑1 (MQ=255)
                                                            ttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGc                                         <  2:2035110/100‑1 (MQ=255)
                                                            ttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGc                                         <  2:2448950/100‑1 (MQ=255)
                                                             tCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGAGCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCg                                        <  1:3069261/100‑1 (MQ=255)
                                                              cgccgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGt                                       >  2:3628734/1‑100 (MQ=255)
                                                               gccgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGtt                                      >  2:3140566/1‑100 (MQ=255)
                                                               gccgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGtt                                      <  1:1232722/100‑1 (MQ=255)
                                                                ccgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTa                                     >  2:255055/1‑100 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                                >  1:1886039/1‑100 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                                <  2:3931536/100‑1 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                                <  1:3682800/100‑1 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                                <  2:2802946/100‑1 (MQ=255)
                                                                        ccttttgtttGGTTGAGGCTTCAATGGGTCTTGGGTCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGTGTTTTTGGCagg                             <  1:829871/91‑1 (MQ=255)
                                                                          tGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggag                           <  2:2161031/100‑1 (MQ=255)
                                                                           ggAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg                          <  1:595405/100‑1 (MQ=255)
                                                                           ggAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCGACGTAGTGTTGCCGAATCACAGTGCGGCGTTACTGGCaggagg                          <  1:149711/100‑1 (MQ=255)
                                                                           ggAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg                          <  2:106139/100‑1 (MQ=255)
                                                                              gggtGGTTGAGGCGTGAATGGAGCGGGAGGGTGATGGCTGGCAAACCTTCCGCTACGTAGTGTGGGCGAATCGCAGTTCGGCGTTACTGGCaggag                           <  1:1958492/93‑1 (MQ=255)
                                                                               tCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATg                      <  1:3975902/100‑1 (MQ=255)
                                                                                cTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGc                     <  2:4134542/100‑1 (MQ=255)
                                                                                 tGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                    >  2:3169530/1‑100 (MQ=255)
                                                                                  ggTTGAGGCGTCAATGGATCTTGGTGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTg                   <  2:3469020/100‑1 (MQ=255)
                                                                                   gTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg                  >  2:3810244/1‑100 (MQ=255)
                                                                                   gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg                  <  2:1844021/100‑1 (MQ=255)
                                                                                    ttGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGc                 <  2:459085/100‑1 (MQ=255)
                                                                                     tGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCg                <  2:1726521/100‑1 (MQ=255)
                                                                                      gAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt               >  1:3270297/1‑100 (MQ=255)
                                                                                      gAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGCCGTTACTCGCAGGAGGCATGCTGGCg                >  1:735811/1‑99 (MQ=255)
                                                                                       aGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGtt              <  2:3521536/100‑1 (MQ=255)
                                                                                       aGGCGTCAATGGCGCTTGGGGCCCATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGtt              >  1:134810/1‑100 (MQ=255)
                                                                                       aGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGtt              >  1:129742/1‑100 (MQ=255)
                                                                                       aGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGtt              >  2:7717/1‑100 (MQ=255)
                                                                                        ggCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt             >  1:1266028/1‑100 (MQ=255)
                                                                                         gCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTg            <  2:2241075/100‑1 (MQ=255)
                                                                                          cGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGc           <  1:1291211/100‑1 (MQ=255)
                                                                                            ttaaTGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc          <  2:347124/97‑1 (MQ=255)
                                                                                            tCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGAATGCTGGCGTTTGCCt         >  1:2768811/1‑100 (MQ=255)
                                                                                            tCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCt         <  2:3752797/100‑1 (MQ=255)
                                                                                             cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCtt        >  1:2871935/1‑100 (MQ=255)
                                                                                             cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCtt        <  1:2844869/100‑1 (MQ=255)
                                                                                             cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCtt        <  2:1538257/100‑1 (MQ=255)
                                                                                              aaTGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTg       >  1:2002702/1‑100 (MQ=255)
                                                                                               aTGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGt      >  1:3910131/1‑100 (MQ=255)
                                                                                                tGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTtctc     >  1:4067190/1‑100 (MQ=255)
                                                                                                 ggATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCg    <  2:1215540/100‑1 (MQ=255)
                                                                                                 ggATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCg    <  2:1609269/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTT  >  NC_000913/1515168‑1515366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: