Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,519,159 A→T 61.2% T45S (ACA→TCA)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,519,1590AT61.2% 85.5 / 138.0 116T45S (ACA→TCA) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (24/21);  new base T (37/34);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

Only 100 of 117 total aligned reads displayed.

CTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCG  >  NC_000913/1519063‑1519255
                                                                                                |                                                                                                
cTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAg                                                                                               >  1:3481744/1‑100 (MQ=255)
cTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAg                                                                                               <  1:100039/100‑1 (MQ=255)
 tGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGt                                                                                              <  2:3541660/100‑1 (MQ=255)
  ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGtt                                                                                             <  1:3282948/100‑1 (MQ=255)
  ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGtt                                                                                             >  2:2509358/1‑100 (MQ=255)
  ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGtt                                                                                             <  1:4136006/100‑1 (MQ=255)
   ggcTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTATTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGttt                                                                                            >  2:2891876/3‑100 (MQ=255)
   gCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGttt                                                                                            >  1:1877060/1‑100 (MQ=255)
    ccTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTa                                                                                           >  1:1199518/1‑100 (MQ=255)
     cTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAt                                                                                          >  1:2921570/1‑100 (MQ=255)
      tCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAtt                                                                                         <  2:2921582/100‑1 (MQ=255)
      tCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAtt                                                                                         <  2:3183921/100‑1 (MQ=255)
      tCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTAtt                                                                                         <  2:3184715/100‑1 (MQ=255)
       cgcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAttt                                                                                        >  2:3719003/1‑100 (MQ=255)
        gcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTg                                                                                       >  2:1096568/1‑100 (MQ=255)
          gTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTc                                                                                     <  2:4040795/100‑1 (MQ=255)
          gTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTc                                                                                     <  2:2713247/100‑1 (MQ=255)
          gTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTc                                                                                     <  1:633493/100‑1 (MQ=255)
             cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCt                                                                                  >  2:3441131/1‑100 (MQ=255)
              ataTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTg                                                                                 >  2:2893408/3‑100 (MQ=255)
                aTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGa                                                                               <  2:1877064/100‑1 (MQ=255)
                 tGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGAc                                                                              >  1:1034889/1‑100 (MQ=255)
                    cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCg                                                                           >  2:3915977/1‑100 (MQ=255)
                    cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCg                                                                           <  2:540638/100‑1 (MQ=255)
                    cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCg                                                                           <  2:1603796/100‑1 (MQ=255)
                      cACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGta                                                                         <  1:968174/100‑1 (MQ=255)
                      cACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGta                                                                         <  1:1217138/100‑1 (MQ=255)
                      cACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGta                                                                         <  1:4117260/100‑1 (MQ=255)
                        ccTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGtata                                                                       <  1:3244050/100‑1 (MQ=255)
                          tGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATg                                                                     <  2:3319201/100‑1 (MQ=255)
                           gtgccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATg                                                                     <  2:3441247/96‑1 (MQ=255)
                           gTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGc                                                                    >  2:3714216/1‑100 (MQ=255)
                           gTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGc                                                                    >  2:378542/1‑100 (MQ=255)
                             tCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGt                                                                  >  1:3408240/1‑100 (MQ=255)
                              ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTg                                                                 <  1:708803/100‑1 (MQ=255)
                               cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTgg                                                                <  1:3655819/100‑1 (MQ=255)
                               cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTgg                                                                >  1:653948/1‑100 (MQ=255)
                                ggAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGa                                                               >  2:1714084/1‑100 (MQ=255)
                                ggAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGa                                                               <  2:2304788/100‑1 (MQ=255)
                                  agaATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACg                                                             <  2:2996567/100‑1 (MQ=255)
                                    aaTTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTa                                                           <  1:1936536/100‑1 (MQ=255)
                                      tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATg                                                         >  1:3113540/1‑100 (MQ=255)
                                      tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATg                                                         >  2:3541821/1‑100 (MQ=255)
                                      tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATg                                                         >  2:272764/1‑100 (MQ=255)
                                        tCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAg                                                       >  1:1091029/1‑100 (MQ=255)
                                          cGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCg                                                     <  2:2507388/100‑1 (MQ=255)
                                           ntCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGa                                                    >  2:1381730/2‑100 (MQ=255)
                                           gTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGGCAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGa                                                    >  1:2272306/1‑100 (MQ=255)
                                           gTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGa                                                    >  1:3321978/1‑100 (MQ=255)
                                           gTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGa                                                    >  2:1936799/1‑100 (MQ=255)
                                           gTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGa                                                    >  1:3602580/1‑100 (MQ=255)
                                            tCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGAt                                                   <  2:4211773/100‑1 (MQ=255)
                                            tCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGAt                                                   <  2:1522836/100‑1 (MQ=255)
                                             cTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATg                                                  <  1:4185324/100‑1 (MQ=255)
                                             cTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCACTGGACCCGTATATGCGTGGACGTATTAGCGCTg                                                  >  1:1630585/1‑100 (MQ=255)
                                               tGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAg                                                <  2:1612477/100‑1 (MQ=255)
                                                gatgaataTGAGGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc                                               <  1:56679/97‑1 (MQ=255)
                                                gaagaaGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc                                               >  1:493756/1‑100 (MQ=255)
                                                 aagaagATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGcc                                              >  2:1150841/1‑100 (MQ=255)
                                                  agaagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCa                                             <  2:2507262/100‑1 (MQ=255)
                                                  agaagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCa                                             >  2:172761/1‑100 (MQ=255)
                                                  agaagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCa                                             <  2:1718147/100‑1 (MQ=255)
                                                  agaagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCa                                             <  2:1715402/100‑1 (MQ=255)
                                                     agaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCTATCt                                          >  1:4143788/1‑100 (MQ=255)
                                                     agaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCt                                          >  1:4031196/1‑100 (MQ=255)
                                                      gatgatGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCtt                                         <  1:3193888/100‑1 (MQ=255)
                                                       atgatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTGGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTa                                        <  2:138324/100‑1 (MQ=255)
                                                        tgatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAt                                       >  1:347807/1‑100 (MQ=255)
                                                        tgatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAt                                       >  1:912239/1‑100 (MQ=255)
                                                          atgTCGCCACACCGGGTGAAGGACAGGTGGTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTc                                     <  1:2760755/100‑1 (MQ=255)
                                                           tgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCa                                    >  2:3955444/1‑100 (MQ=255)
                                                           tgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCa                                    >  2:3337805/1‑100 (MQ=255)
                                                              cGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCg                                 >  1:2286621/1‑100 (MQ=255)
                                                               gCCACACCGGGTGAGGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGc                                <  2:2459200/100‑1 (MQ=255)
                                                               gCCACACCGGGTGAAGGATAGGTGTTACTGCGCTCAGTTTATTTGTCCCTTGACTCGTATGTGCGTGGACTTATGAGCGATGAGCCATCTTATTCAcc                                  >  1:3479646/1‑98 (MQ=255)
                                                               gCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGc                                <  1:2202485/100‑1 (MQ=255)
                                                               gCCACACCGGGTGAAGGACAGGTGTTACTGCACACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGc                                <  1:765785/100‑1 (MQ=255)
                                                                 cacaCCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTGATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCt                              >  1:2286461/1‑100 (MQ=255)
                                                                 cacaCCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCt                              >  2:360709/1‑100 (MQ=255)
                                                                     ccGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTg                          >  1:1277040/1‑100 (MQ=255)
                                                                        ggTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCTCCGCCTGTTGaga                       <  2:2877815/100‑3 (MQ=255)
                                                                         gTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGatat                      >  2:1478758/1‑100 (MQ=255)
                                                                             aGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggc                  >  1:2348690/1‑100 (MQ=255)
                                                                              ggACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGTCATCTTATTCACCGCCTGTTGATATTggcg                 >  1:4090002/1‑100 (MQ=255)
                                                                               gACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcgg                <  2:1377157/100‑1 (MQ=255)
                                                                               gACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcgg                >  2:3796162/1‑100 (MQ=255)
                                                                               gACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGACATCTTATTCACCGCCTGTTGATATTggcgg                >  2:2449263/1‑100 (MQ=255)
                                                                                aCAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcggc               >  1:1543836/1‑100 (MQ=255)
                                                                                 cAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg              <  2:192293/100‑1 (MQ=255)
                                                                                 cAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg              <  2:1094712/100‑1 (MQ=255)
                                                                                   ggTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg            >  2:2513669/1‑100 (MQ=255)
                                                                                   ggTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg            >  2:1027861/1‑100 (MQ=255)
                                                                                   ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg            >  2:3392396/1‑100 (MQ=255)
                                                                                   ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg            <  2:3378059/100‑1 (MQ=255)
                                                                                    gtgtTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGa           <  2:2296311/100‑1 (MQ=255)
                                                                                         aCTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTc      <  2:2286467/100‑1 (MQ=255)
                                                                                          cTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcg     >  2:3088496/1‑100 (MQ=255)
                                                                                            gcgcTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggc   <  1:2588903/100‑1 (MQ=255)
                                                                                             cgcACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggcg  <  2:4273344/100‑1 (MQ=255)
                                                                                                acaGTTTATTTGTCCCTGGAGCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTTTTATTGGCGGCGTGATGGTcggcg  <  1:200317/97‑1 (MQ=255)
                                                                                                |                                                                                                
CTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCG  >  NC_000913/1519063‑1519255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑