Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,665,195 (T)7→8 intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,665,1881.T95.6% 234.3 / 3.7 68intergenic (+68/‑127)dmsD/clcBtwin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base T (26/39);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGC  >  NC_000913/1665089‑1665282
                                                                                                    |                                                                                              
ttCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACg                                                                                                 <  2:716248/100‑1 (MQ=255)
ttCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACg                                                                                                 >  2:181049/1‑100 (MQ=255)
  ccTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtt                                                                                              >  1:1692999/1‑100 (MQ=255)
    tGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttt                                                                                            >  1:542839/1‑100 (MQ=255)
      tCGCGGTTAAACCGCTGTTTCGATAGGATCACCGGAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttttt                                                                                          <  2:1070310/100‑1 (MQ=255)
       cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         <  1:349931/100‑1 (MQ=255)
       cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         <  1:702233/100‑1 (MQ=255)
       cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         >  2:246040/1‑100 (MQ=255)
       cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         >  2:1030868/1‑100 (MQ=255)
       cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         <  2:1360264/100‑1 (MQ=255)
        gcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCGATCCGGCAATAATAGGTTACAGTGTCACGtttttttt                                                                                        <  2:1253216/100‑2 (MQ=255)
        gcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGACGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                                                         <  2:1949309/99‑1 (MQ=255)
              aaaCCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATctct                                                                                   >  1:1543022/1‑100 (MQ=255)
               aaCCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCtt                                                                                  >  1:389049/1‑100 (MQ=255)
                   gCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAg                                                                              >  2:678587/1‑100 (MQ=255)
                   gCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAg                                                                              <  2:87513/100‑1 (MQ=255)
                   gCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgc                                                                             <  2:2126363/100‑1 (MQ=255)
                     tGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgca                                                                            <  1:1995193/100‑1 (MQ=255)
                      gTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcac                                                                           >  2:848972/1‑100 (MQ=255)
                        ttCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgc                                                                         <  1:1043228/100‑1 (MQ=255)
                        ttCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgc                                                                         <  2:2118049/100‑1 (MQ=255)
                           gATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACt                                                                      >  1:260827/1‑100 (MQ=255)
                           gATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACt                                                                      <  2:2292239/100‑1 (MQ=255)
                            aTAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACt                                                                      <  1:277161/99‑1 (MQ=255)
                             tAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGc                                                                    >  1:2308062/1‑100 (MQ=255)
                               ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                  >  2:2115490/1‑100 (MQ=255)
                               ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                  >  1:2107197/1‑100 (MQ=255)
                               ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                  >  1:1975079/1‑100 (MQ=255)
                                gATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCttt                                                                 >  1:369769/1‑100 (MQ=255)
                                 aTCACCGTAATATTGCCGGATGGTGACGATTACGAGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACAGAtttt                                                                >  1:1013648/1‑100 (MQ=255)
                                     ccGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                                                            >  1:395732/1‑100 (MQ=255)
                                     ccGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                                                            <  1:814249/100‑1 (MQ=255)
                                       gTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCt                                                          <  2:1013646/100‑1 (MQ=255)
                                       gTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGAAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCt                                                          >  2:1444025/1‑100 (MQ=255)
                                        tAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                         <  2:2307913/100‑1 (MQ=255)
                                         aaTATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg                                                        >  2:438273/1‑100 (MQ=255)
                                           tatTGTCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcn                                                      <  2:1556222/100‑2 (MQ=255)
                                           tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                      <  1:893297/100‑1 (MQ=255)
                                           tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                      <  1:968768/100‑1 (MQ=255)
                                           tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                      >  2:1769658/1‑100 (MQ=255)
                                           tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                      <  1:1769663/100‑1 (MQ=255)
                                               gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtt                                                  <  2:2064679/100‑1 (MQ=255)
                                               gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt                                                 >  2:981497/1‑100 (MQ=255)
                                                ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt                                                 <  2:260825/100‑1 (MQ=255)
                                                ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt                                                 <  1:1962346/100‑1 (MQ=255)
                                                     tGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCg                                            <  1:857792/100‑1 (MQ=255)
                                                     tGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCg                                            <  1:794363/100‑1 (MQ=255)
                                                     tGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCg                                            <  2:791986/100‑1 (MQ=255)
                                                      ggTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGc                                           <  2:2244750/100‑1 (MQ=255)
                                                        tGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTTTCTTTTTTAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaa                                         >  2:1240152/1‑100 (MQ=255)
                                                         gACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaa                                        >  2:1880474/1‑100 (MQ=255)
                                                           cGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAAt                                      <  1:1477102/100‑1 (MQ=255)
                                                             aTTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAg                                    <  2:47842/100‑1 (MQ=255)
                                                                 cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCg                                 <  1:678588/99‑1 (MQ=255)
                                                                 cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc                                <  1:1393157/100‑1 (MQ=255)
                                                                   cgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc                              >  2:560060/1‑100 (MQ=255)
                                                                   cgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATATCTTAAAGCACGCACTGCTTTTTCGACTGGCCTCTTTTGCGGCAAAATCGt                                   >  2:1747954/1‑95 (MQ=255)
                                                                    gcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCg                             >  1:2331047/1‑100 (MQ=255)
                                                                         ccGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt                        <  2:1212100/100‑1 (MQ=255)
                                                                          cGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTc                       <  2:2063890/100‑1 (MQ=255)
                                                                           ggCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa                      <  1:1784368/100‑1 (MQ=255)
                                                                           ggCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa                      >  2:1459025/1‑100 (MQ=255)
                                                                           ggCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAt                     >  1:37798/1‑100 (MQ=255)
                                                                             cAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAtt                    <  1:560061/100‑1 (MQ=255)
                                                                              aataatAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTg                   <  2:1975072/100‑1 (MQ=255)
                                                                               ataataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc                  <  2:395730/100‑1 (MQ=255)
                                                                               ataataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc                  <  1:990813/100‑1 (MQ=255)
                                                                                 aataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGccc                <  1:1444029/100‑1 (MQ=255)
                                                                                   taGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTTCCCAt              >  2:301395/1‑100 (MQ=255)
                                                                                     ggTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAttt            >  2:1259244/1‑100 (MQ=255)
                                                                                        tACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg         >  1:965057/1‑100 (MQ=255)
                                                                                           aGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTc      <  1:1332362/100‑1 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCCCTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGACCGTGTTTCATTGCCCATTTCTGCTCAt    <  1:1154291/99‑1 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg   <  2:2308053/100‑1 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg   >  1:643424/1‑100 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg   <  2:1692994/100‑1 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg   >  1:380020/1‑100 (MQ=255)
                                                                                              gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg   <  2:1858280/100‑1 (MQ=255)
                                                                                               tCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGc  <  1:216886/100‑1 (MQ=255)
                                                                                               tCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGc  <  2:369767/100‑1 (MQ=255)
                                                                                                    |                                                                                              
TTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGC  >  NC_000913/1665089‑1665282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: