Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T96.1% 356.0 / 6.9 103coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base T (44/55);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 120 total aligned reads displayed.

GTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469553‑2469743
                                                                                                 |                                                                                              
gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTTTTTTATTTTTTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttt                                                                                             >  2:1914911/1‑100 (MQ=255)
 tttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttt                                                                                            <  2:1650882/100‑1 (MQ=255)
  ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt                                                                                           >  2:458143/1‑100 (MQ=255)
    gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  1:486998/1‑100 (MQ=255)
    gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         <  1:960377/100‑1 (MQ=255)
     catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTTTTTTTTTTTTTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                        >  2:332468/1‑99 (MQ=255)
     catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                        >  1:1739706/1‑99 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       <  1:2324807/100‑3 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       >  1:2068072/1‑98 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       <  1:1470064/100‑3 (MQ=255)
      atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       <  1:75038/100‑3 (MQ=255)
       tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCGATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcg                                                                                      >  2:1255011/1‑97 (MQ=255)
       tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcg                                                                                      <  1:2063469/100‑4 (MQ=255)
          ttCTTTTAAGAGCAAATAGTTATAATTTTCCTGCCATGGTTTGTGCATTAAAAAAAAAAAAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     <  2:2223448/98‑5 (MQ=255)
          ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                    <  1:432425/100‑1 (MQ=255)
           tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  1:53383/100‑1 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  1:2229300/1‑100 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  1:1845861/1‑100 (MQ=255)
              aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  2:2260921/1‑100 (MQ=255)
                cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTGTtaata                                                                              >  2:541320/1‑100 (MQ=255)
                cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              <  2:921113/100‑1 (MQ=255)
                 aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             >  2:58208/1‑100 (MQ=255)
                 aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             <  1:2260929/100‑1 (MQ=255)
                  agagCAAATAGTTATAATTTTTCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  1:1257554/100‑1 (MQ=255)
                   gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  1:2184129/100‑1 (MQ=255)
                        aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                      >  1:1730517/1‑100 (MQ=255)
                        aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                      >  1:1652827/1‑100 (MQ=255)
                         aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  2:16420/100‑1 (MQ=255)
                         aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  2:18517/100‑1 (MQ=255)
                             ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTa                                                                 >  2:1540138/1‑100 (MQ=255)
                               ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               <  1:1204454/100‑1 (MQ=255)
                               ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               >  1:852336/1‑100 (MQ=255)
                               ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               >  1:1189579/1‑100 (MQ=255)
                               ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               >  1:204926/1‑100 (MQ=255)
                                taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              <  2:1936749/100‑1 (MQ=255)
                                taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              <  1:2304419/100‑1 (MQ=255)
                                taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              >  1:716651/1‑100 (MQ=255)
                                  aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:1372339/100‑1 (MQ=255)
                                  aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:858974/100‑1 (MQ=255)
                                    tttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                          >  1:2319518/1‑100 (MQ=255)
                                      tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  2:778526/100‑1 (MQ=255)
                                      tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  1:1267949/100‑1 (MQ=255)
                                      tccttccATTTTTGGTGTATACATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  2:2041179/100‑1 (MQ=255)
                                         ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     <  2:486996/100‑1 (MQ=255)
                                          tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    <  1:1698193/100‑1 (MQ=255)
                                          tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    <  1:819069/100‑1 (MQ=255)
                                            cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                  >  1:928100/1‑100 (MQ=255)
                                                tttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAc                                              >  1:483955/1‑100 (MQ=255)
                                                 ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             >  1:1272359/1‑100 (MQ=255)
                                                 ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:204924/100‑1 (MQ=255)
                                                 ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:2229292/100‑1 (MQ=255)
                                                   ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCCATTTATATTAAcgcc                                           >  2:85953/1‑100 (MQ=255)
                                                   ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTCTTTTTATATATACTTCTAAAAATTAATTTatat                                                  >  2:774443/1‑92 (MQ=255)
                                                      gtATTCATTAACATGCAAAAACAATAATTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  1:1285831/99‑1 (MQ=255)
                                                      gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  2:912229/100‑1 (MQ=255)
                                                      gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  2:1132099/100‑1 (MQ=255)
                                                      gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  2:1035981/100‑1 (MQ=255)
                                                       tATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTa                                       <  1:340963/100‑1 (MQ=255)
                                                         ttCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     >  2:1601480/1‑100 (MQ=255)
                                                          tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    >  2:1895496/1‑100 (MQ=255)
                                                          tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    <  2:1189576/100‑1 (MQ=255)
                                                           cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    >  2:1501286/1‑99 (MQ=255)
                                                           cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  2:2187303/1‑100 (MQ=255)
                                                           cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  1:2076997/100‑1 (MQ=255)
                                                           cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  2:613573/1‑100 (MQ=255)
                                                            aTTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACACCACTtata                                    >  2:2138117/1‑98 (MQ=255)
                                                              tAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtgt                                >  1:1454511/1‑100 (MQ=255)
                                                               aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgagat                               >  1:299353/1‑96 (MQ=255)
                                                                 cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             >  2:513784/1‑100 (MQ=255)
                                                                 cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             <  1:513786/100‑1 (MQ=255)
                                                                  aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                            >  1:1186194/1‑100 (MQ=255)
                                                                   tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           >  2:805180/1‑100 (MQ=255)
                                                                   tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           >  2:1396708/1‑100 (MQ=255)
                                                                    gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:1486053/1‑100 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  2:663043/1‑100 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  2:1976428/1‑100 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         <  2:1272356/100‑1 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  1:1560928/1‑100 (MQ=255)
                                                                     cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTAGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         <  1:1639590/100‑1 (MQ=255)
                                                                        aaaCAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTTTAATATCTACTGCTAAAAATTAATTTATATTAACCCCACTAATAGTGTATTCttatt                       >  2:790841/1‑99 (MQ=255)
                                                                         aaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATTATTTTATATTAACCCCACTATTATTTTTTTCatattat                     >  2:850761/1‑100 (MQ=255)
                                                                            aaTAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatatt                  <  1:604400/100‑1 (MQ=255)
                                                                               aaaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttt               <  1:1016185/100‑1 (MQ=255)
                                                                                 aTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             <  2:1486049/100‑1 (MQ=255)
                                                                                  tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            >  2:574767/1‑100 (MQ=255)
                                                                                  tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:1194435/100‑1 (MQ=255)
                                                                                   cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  2:1913635/100‑1 (MQ=255)
                                                                                   cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  1:280788/100‑1 (MQ=255)
                                                                                   cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  1:453520/100‑1 (MQ=255)
                                                                                    tATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGa          <  1:1437209/100‑1 (MQ=255)
                                                                                      tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  2:917769/100‑1 (MQ=255)
                                                                                      tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  2:9428/100‑1 (MQ=255)
                                                                                       gCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCATTAATAGTGTATTCATATTATATTTTTTTTGAt         >  2:1222688/1‑98 (MQ=255)
                                                                                        cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg       <  2:1533340/99‑1 (MQ=255)
                                                                                        cTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac     <  1:1153336/100‑1 (MQ=255)
                                                                                          tttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaca    <  2:1845856/100‑1 (MQ=255)
                                                                                          tttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaca    >  1:2275583/1‑100 (MQ=255)
                                                                                           ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCTCTAATAGTGTATTCATATTATATTTTTTTTGATAGacac   <  1:458145/100‑1 (MQ=255)
                                                                                           ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGacac   <  2:510733/100‑1 (MQ=255)
                                                                                            tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  <  2:1987517/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469553‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: