Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,434,4590CT79.2% 22.4 / 7.0 24intergenic (‑235/+499)ynaE/ttcCcold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (5/14);  total (8/16)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGC  >  NC_000913/1434362‑1434539
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gCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTg                                                                                <  1:1603018/100‑1 (MQ=25)
 cTGAATATGATAAGGAGCGAAATGATTATAAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTgg                                                                               <  2:1048016/100‑1 (MQ=255)
  tGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTggg                                                                              <  1:1960136/100‑1 (MQ=25)
   gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt                                                                             <  1:1474158/100‑1 (MQ=18)
   gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt                                                                             >  1:1670887/1‑100 (MQ=18)
   gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt                                                                             >  1:1212412/1‑100 (MQ=18)
   gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGgt                                                                             >  2:530456/1‑100 (MQ=255)
    aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtg                                                                            <  1:963420/100‑1 (MQ=17)
    aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtg                                                                            <  2:63724/100‑1 (MQ=14)
    aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtg                                                                            <  1:2268937/100‑1 (MQ=18)
        tGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCt                                                                        <  2:259545/100‑1 (MQ=18)
        tGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCt                                                                        >  2:626648/1‑100 (MQ=18)
        tGATAAAGAGCGAAGTTATTATCAGTATTCTGTTCATATAGCCTCTAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCt                                                                        <  2:1393935/100‑1 (MQ=11)
         gATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGAAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCtt                                                                       >  2:411964/1‑100 (MQ=255)
          aTAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTa                                                                      <  1:2030854/100‑1 (MQ=18)
          aTAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTa                                                                      >  1:2015378/1‑100 (MQ=18)
              ggAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGt                                                                  <  1:1759450/100‑1 (MQ=17)
               gAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGtt                                                                 <  1:745340/100‑1 (MQ=14)
                aGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGTTc                                                                <  2:1656816/100‑1 (MQ=14)
                  cGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGTTCGt                                                              <  2:908986/100‑1 (MQ=1)
                           tATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACa                                                     <  2:998523/100‑1 (MQ=33)
                                          cATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGta                                      >  1:778004/1‑100 (MQ=32)
                                             ataGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATAc                                   >  2:1540067/1‑100 (MQ=32)
                                                                              ataGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGc  <  1:1945215/100‑1 (MQ=38)
                                                                                                 |                                                                                
GCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGC  >  NC_000913/1434362‑1434539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: