Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,740	G→T	G150C (GGT→TGT)	rihC →	ribonucleoside hydrolase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,740	0	G	T	100.0%	196.4 / NA	56	G150C (GGT→TGT)	rihC	ribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/25);  total (31/25)

TGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGTGGTATTG  >  NC_000913/27643‑27836                                                                                                  |                                                                                                 tGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATgtgt                                                                                                >  2:2051860/1‑100 (MQ=255)    tACCCTGGTGGCCATCGGCCCGTTCACCAATATTGCGCTGTTACTCTCACACTGCGCGGAATGCAATACTTATATTCGACGTTAGATGCTCATGagtg                                                                                               >  2:194218/1‑98 (MQ=255)    tACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGtgtggt                                                                                             <  2:1553874/100‑1 (MQ=255)    tACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGtgtggt                                                                                             >  2:175936/1‑100 (MQ=255)     aCCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGtt                                                                                            <  2:1695370/100‑1 (MQ=255)       ccTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCt                                                                                          >  2:1204014/1‑100 (MQ=255)         tggtggCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGc                                                                                        <  1:1204017/100‑1 (MQ=255)           gtggCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCg                                                                                      <  1:231703/100‑1 (MQ=255)            tggCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCgg                                                                                     <  2:1957891/100‑1 (MQ=255)               ccATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGAcg                                                                                  >  1:1593085/1‑100 (MQ=255)                 tcttccgatctAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGAcgcg                                                                                <  1:81237/90‑1 (MQ=255)                  tCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCgg                                                                               >  2:1462534/1‑100 (MQ=255)                    ggCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCa                                                                             <  2:563733/100‑1 (MQ=255)                    ggCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCa                                                                             <  2:1198453/100‑1 (MQ=255)                     gCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCaa                                                                            >  2:1085165/1‑100 (MQ=255)                       ccGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACt                                                                          <  2:275335/100‑1 (MQ=255)                       ccGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACt                                                                          <  2:277390/100‑1 (MQ=255)                              ccAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGcc                                                                   >  2:1135956/1‑100 (MQ=255)                              ccAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGcc                                                                   >  1:440219/1‑100 (MQ=255)                              ccAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGcc                                                                   <  1:2140641/100‑1 (MQ=255)                                     tGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGcc                                                            >  2:1166195/1‑100 (MQ=255)                                     tGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGcc                                                            >  2:979848/1‑100 (MQ=255)                                        gcTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAg                                                         >  1:1652841/1‑100 (MQ=255)                                        gcTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAg                                                         >  1:960202/1‑100 (MQ=255)                                         cTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGt                                                        <  1:2051867/100‑1 (MQ=255)                                           gTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGttt                                                      >  2:2197290/1‑100 (MQ=255)                                           gTTACGTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGtct                                                      <  2:1470786/100‑3 (MQ=255)                                             tACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTaa                                                    <  1:1133307/100‑1 (MQ=255)                                              aCTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAat                                                   <  2:1374165/100‑1 (MQ=255)                                               cTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAata                                                  >  2:898023/1‑100 (MQ=255)                                               cTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAata                                                  <  2:959474/100‑1 (MQ=255)                                                   cacaATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATtgc                                              >  2:270001/1‑100 (MQ=255)                                                    acaATGCCCGGAATACAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATtgct                                             <  2:1593080/100‑1 (MQ=255)                                                       aTGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGcc                                          <  1:175938/100‑1 (MQ=255)                                                          cccGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGAt                                       >  1:1961723/1‑100 (MQ=255)                                                            cGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATcc                                     >  1:1558530/1‑100 (MQ=255)                                                            cGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCCAGTTTAATATTGCTGCCGATcc                                     <  2:1360259/100‑1 (MQ=255)                                                              gAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAg                                   >  2:1775576/1‑100 (MQ=255)                                                                 tGCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAg                                <  2:1170280/100‑1 (MQ=255)                                                                  gCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAgc                               >  1:2019342/1‑100 (MQ=255)                                                                  gCAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAgc                               >  2:2057756/1‑100 (MQ=255)                                                                   cAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAgct                              <  1:2091156/100‑1 (MQ=255)                                                                   cAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAgct                              >  1:293815/1‑100 (MQ=255)                                                                   cAAGCCGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAgct                              >  2:2160310/1‑100 (MQ=255)                                                                        cGTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCtgctgc                         >  1:487660/1‑100 (MQ=255)                                                                         gTATATTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGcc                        <  2:960200/100‑1 (MQ=255)                                                                           atatTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCtg                      >  1:807266/1‑100 (MQ=255)                                                                            tatTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCtgt                     >  2:2193868/1‑100 (MQ=255)                                                                             atTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCtgtg                    <  2:649107/100‑1 (MQ=255)                                                                             atTCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCtgtg                    <  2:8068/100‑1 (MQ=255)                                                                              ttCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTgtgt                   >  2:283661/1‑100 (MQ=255)                                                                              ttCGCCGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTgtgt                   >  2:286753/1‑100 (MQ=255)                                                                               tCGCCGTCTGGTGATCATGTGTGGTTCTGCCGTACGCGGCAACTGTACGGCAAACGCCGAGTTTAATATTGCTGCCGATTCAGAAGCTGCTGCCTGTGTc                  <  2:1612814/100‑1 (MQ=255)                                                                                   cGTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTcc              <  1:1887219/100‑1 (MQ=255)                                                                                    gTCTGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCg             >  1:1553967/1‑100 (MQ=255)                                                                                       tGGTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAg          >  1:1250821/1‑100 (MQ=255)                                                                                        ggTGATCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGt         >  1:1167730/1‑100 (MQ=255)                                                                                             tCATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGTGGTAt    >  1:1033317/1‑100 (MQ=255)                                                                                              cATGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGTGGTAtt   >  1:1124325/1‑100 (MQ=255)                                                                                               aTGTGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGTGGTATTg  >  2:1555230/1‑100 (MQ=255)                                                                                                  |                                                                                                 TGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGCCGGACGCGGCAACTGTACGCCAAACGCCGAGTTTAATATTGCTGCCGATCCAGAAGCTGCTGCCTGTGTCTTCCGCAGTGGTATTG  >  NC_000913/27643‑27836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑