| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,490,055 | T→G | E3D (GAA→GAC) | yjgR ← | DUF853 family protein with NTPase fold |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,490,055 | 0 | T | G | 96.1% | 271.1 / ‑0.8 | 77 | E3D (GAA→GAC) | yjgR | DUF853 family protein with NTPase fold |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base G (38/36); total (41/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150 | tACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTc > 2:1936045/1‑100 (MQ=255) ccccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATg < 2:2081911/100‑1 (MQ=255) ccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgag < 1:79597/100‑1 (MQ=255) cGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgaga < 2:2225774/100‑1 (MQ=255) cgcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcc > 1:1410858/1‑100 (MQ=255) gcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct > 2:422619/1‑100 (MQ=255) gTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAg < 2:384584/100‑1 (MQ=255) aaTCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGtt > 2:2050707/1‑100 (MQ=255) cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc < 2:1547545/100‑1 (MQ=255) cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc < 2:2284745/100‑1 (MQ=255) gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAggcaggaa > 1:659873/1‑93 (MQ=255) gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg > 2:1551377/1‑100 (MQ=255) cccATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgc > 2:2291909/1‑100 (MQ=255) cttccgatcttAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcc < 2:1939328/91‑1 (MQ=255) ccATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcc < 1:1936052/100‑1 (MQ=255) ccATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcc < 1:2291051/100‑1 (MQ=255) cATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcct < 1:23794/100‑1 (MQ=255) tGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCTATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgcctg < 2:1076631/99‑1 (MQ=255) tGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCAGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTgg < 2:1646679/100‑1 (MQ=255) gTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtat > 1:1208623/1‑100 (MQ=255) ttAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtata < 2:680146/100‑1 (MQ=255) ttAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtata < 1:376112/100‑1 (MQ=255) tAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgccagatcggaag > 1:1939335/1‑90 (MQ=255) atctcATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAg < 1:980577/96‑1 (MQ=255) aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg > 1:485376/1‑100 (MQ=255) aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 2:1747490/100‑1 (MQ=255) aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGAGCAATTAACAGGAGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 2:1640233/100‑1 (MQ=255) gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc > 1:2252471/1‑100 (MQ=255) gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc > 1:881885/1‑100 (MQ=255) gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTAATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg > 1:1853827/1‑99 (MQ=255) cgatctCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc < 1:1049982/95‑1 (MQ=255) cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgagat > 2:980575/1‑96 (MQ=255) cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCaa > 1:1258733/1‑100 (MQ=255) cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTACAGTTTCCTGCCTGGAGTATAGGCaa > 1:833500/1‑100 (MQ=255) cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCagatcg > 2:1049980/1‑95 (MQ=255) cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACggc > 2:1415901/1‑98 (MQ=255) ccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTcc < 1:896342/100‑1 (MQ=255) cAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCa < 1:2050714/100‑1 (MQ=255) gTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCaaa > 2:1262079/1‑100 (MQ=255) tAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAag > 1:211453/1‑100 (MQ=255) tAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAag < 2:833498/100‑1 (MQ=255) tAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAag < 2:1208620/100‑1 (MQ=255) aaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAaga > 2:2165527/1‑100 (MQ=255) aaaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAaga > 1:1307654/1‑100 (MQ=255) aaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAagag > 1:1983763/1‑100 (MQ=255) aaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAagag < 2:1235789/100‑1 (MQ=255) aCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAAt < 1:1671549/100‑1 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa > 2:144836/1‑100 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa < 2:1983756/100‑1 (MQ=255) gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATTAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg < 2:1853822/100‑1 (MQ=255) gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg < 1:422621/100‑1 (MQ=255) gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg < 1:1501773/100‑1 (MQ=255) gTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACg > 1:722844/1‑100 (MQ=255) tCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACgtg > 1:1145340/1‑100 (MQ=255) gtgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTaa > 2:271901/1‑100 (MQ=255) gtgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTaa > 2:872729/1‑100 (MQ=255) gtgtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTaa > 2:1880459/1‑100 (MQ=255) gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg > 1:1980059/1‑100 (MQ=255) gtCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATg > 2:1775477/1‑100 (MQ=255) tCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGa < 2:140515/100‑1 (MQ=255) tCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGa > 1:1612456/1‑100 (MQ=255) ccGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGaa < 1:775963/100‑1 (MQ=255) ccGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGaa < 1:775884/100‑1 (MQ=255) cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag < 1:2243636/100‑1 (MQ=255) cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAag > 1:800531/1‑100 (MQ=255) gCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAAGGAAGAGaaaa > 1:2048362/1‑100 (MQ=255) cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt > 1:543677/1‑100 (MQ=255) gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGTAGAGAAAATa > 2:2214152/1‑98 (MQ=255) gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc < 1:335703/100‑1 (MQ=255) cAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAcc > 2:793250/1‑100 (MQ=255) ttAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaa < 1:1308008/100‑1 (MQ=255) tAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTaaa > 1:2105778/1‑100 (MQ=255) tAACAGGGGGTCACTCATGAGATATCCTCCAGTGTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAATATAACCTaaa > 2:1533880/1‑100 (MQ=255) aCAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGc > 2:2069356/1‑100 (MQ=255) cAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCg < 2:2252463/100‑1 (MQ=255) cAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCg < 2:1145338/100‑1 (MQ=255) ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg > 1:1168340/1‑100 (MQ=255) | TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |