Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 606,299 A→T V362E (GTG→GAG)  ybdK ← weak gamma‑glutamyl:cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913606,2990AT97.5% 423.9 / ‑0.8 122V362E (GTG→GAG) ybdKweak gamma‑glutamyl:cysteine ligase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (55/63);  total (56/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CATTGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATC  >  NC_000913/606202‑606394
                                                                                                 |                                                                                               
cATTGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTcc                                                                                               <  1:2014818/100‑1 (MQ=255)
cATTGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTcc                                                                                               <  1:1810846/100‑1 (MQ=255)
atctGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTcc                                                                                               <  2:388473/97‑1 (MQ=255)
 aTTGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCa                                                                                              >  2:2165048/1‑100 (MQ=255)
  ttGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAg                                                                                             >  2:343996/1‑100 (MQ=255)
  ttGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAg                                                                                             >  2:1979417/1‑100 (MQ=255)
  ttGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAg                                                                                             <  1:1767387/100‑1 (MQ=255)
   tGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGc                                                                                            >  2:1827277/1‑100 (MQ=255)
    gTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAgat                                                                                           >  1:388475/1‑98 (MQ=255)
    gTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGcc                                                                                           >  2:1940903/1‑100 (MQ=255)
    gTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGcc                                                                                           >  1:245096/1‑100 (MQ=255)
     ttAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGccc                                                                                          <  2:739958/100‑1 (MQ=255)
      tAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCa                                                                                         >  1:633033/1‑100 (MQ=255)
             aaGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCg                                                                                  <  2:911819/100‑1 (MQ=255)
             aaGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCg                                                                                  <  1:2139416/100‑1 (MQ=255)
              aGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGa                                                                                 >  2:304771/1‑100 (MQ=255)
              aGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGa                                                                                 >  1:2233206/1‑100 (MQ=255)
              aGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGa                                                                                 <  2:406202/100‑1 (MQ=255)
               gTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGa                                                                                 >  2:1511672/1‑99 (MQ=255)
               gTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAg                                                                                >  2:154258/1‑100 (MQ=255)
                 tAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCAGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgcc                                                                              >  2:634976/1‑100 (MQ=255)
                  aaaaCACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgccg                                                                             >  2:121248/1‑100 (MQ=255)
                   aaaCACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgc                                                                               >  1:842002/1‑97 (MQ=255)
                   aaaCACCATTGTCAGGGAAATTCTTCTGTAAGTCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgccgc                                                                            >  1:2211867/1‑100 (MQ=255)
                    aaCACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgccgcc                                                                           >  1:1015667/1‑100 (MQ=255)
                    aaCACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAgccgc                                                                            <  2:2232620/99‑1 (MQ=255)
                     acacCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCa                                                                          <  1:1529328/100‑1 (MQ=255)
                      cacCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCAt                                                                         >  1:228806/1‑100 (MQ=255)
                        ccATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCg                                                                       >  1:662041/1‑100 (MQ=255)
                        ccATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCg                                                                       >  2:762687/1‑100 (MQ=255)
                        ccATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCg                                                                       >  1:1244691/1‑100 (MQ=255)
                        ccATTGTCAGGGAAATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCg                                                                       <  2:2211859/100‑1 (MQ=255)
                         cATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCgg                                                                      <  1:1979424/100‑1 (MQ=255)
                          aTTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGc                                                                     <  2:1145646/100‑1 (MQ=255)
                           ttGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGcg                                                                    <  1:1769217/100‑1 (MQ=255)
                             gTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGcgac                                                                  <  1:701438/100‑1 (MQ=255)
                             gTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGcgac                                                                  <  1:763093/100‑1 (MQ=255)
                             gTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGcgac                                                                  >  1:1113249/1‑100 (MQ=255)
                              tCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGcgacg                                                                 <  2:1876379/100‑1 (MQ=255)
                               cAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCACCATCGGcaacg                                                                 >  1:1315301/1‑99 (MQ=255)
                                aGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGaa                                                               >  2:393179/1‑100 (MQ=255)
                                  ggATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAAt                                                             >  2:871704/1‑100 (MQ=255)
                                  ggATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAAt                                                             <  1:203302/100‑1 (MQ=255)
                                  ggATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAAt                                                             <  1:203396/100‑1 (MQ=255)
                                    atatTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATcg                                                           <  2:1005801/100‑1 (MQ=255)
                                    atatTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATcg                                                           <  1:304773/100‑1 (MQ=255)
                                    atatTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATcg                                                           >  2:2214831/1‑100 (MQ=255)
                                    atatTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATcg                                                           >  2:2311961/1‑100 (MQ=255)
                                     tatTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATcgc                                                          >  1:2092073/1‑100 (MQ=255)
                                       ttcttcTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCgcgc                                                        >  2:2327360/1‑100 (MQ=255)
                                        tcttctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCa                                                       <  2:295732/100‑1 (MQ=255)
                                        tcttctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCa                                                       >  2:1002032/1‑100 (MQ=255)
                                         cttctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCAt                                                      >  2:1781637/1‑100 (MQ=255)
                                            ctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAg                                                   <  2:245094/100‑1 (MQ=255)
                                            ctGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAg                                                   >  2:20685/1‑100 (MQ=255)
                                             tGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGc                                                  <  1:1756830/100‑1 (MQ=255)
                                              gTAAGGCAATTCCCGGCTTAGTCACCGGCCCATATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCt                                                 <  1:1394077/100‑1 (MQ=255)
                                              gTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCt                                                 >  1:1869130/1‑100 (MQ=255)
                                                aaGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTgc                                               <  1:44313/100‑1 (MQ=255)
                                                aaGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTgc                                               <  1:2311970/100‑1 (MQ=255)
                                                 aGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTgcg                                              <  1:242113/100‑1 (MQ=255)
                                                  ggCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTgcgc                                             >  2:1519148/1‑100 (MQ=255)
                                                   gCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCt                                            <  1:248515/100‑1 (MQ=255)
                                                   gCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCt                                            <  2:1548364/100‑1 (MQ=255)
                                                   gCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCt                                            <  2:662040/100‑1 (MQ=255)
                                                    cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCtt                                           <  1:2046564/100‑1 (MQ=255)
                                                    cAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCtt                                           <  2:1244688/100‑1 (MQ=255)
                                                       ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCttcat                                        <  1:1737322/100‑1 (MQ=255)
                                                       ttCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCttcat                                        <  2:1602617/100‑1 (MQ=255)
                                                         cccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCttcattc                                      <  1:1379437/100‑1 (MQ=255)
                                                          ccGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCttcattca                                     <  1:343998/100‑1 (MQ=255)
                                                           cGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAg                                    <  2:1025912/100‑1 (MQ=255)
                                                            ggCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGa                                   >  2:969041/1‑100 (MQ=255)
                                                             gCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGAc                                  <  2:951080/100‑1 (MQ=255)
                                                              cTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGAc                                  <  1:2243367/99‑1 (MQ=255)
                                                               ttAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCg                                >  2:1916585/1‑100 (MQ=255)
                                                               ttAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCg                                <  1:120881/100‑1 (MQ=255)
                                                               ttAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCg                                <  2:2221061/100‑1 (MQ=255)
                                                                tAGTCACCGGCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGc                               >  1:1008442/1‑100 (MQ=255)
                                                                        ggCCCAGATCTCACAATGTTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGAcc                       <  1:217045/100‑1 (MQ=255)
                                                                        ggCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGAcc                       <  1:819357/100‑1 (MQ=255)
                                                                         gCCCAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCt                      <  1:969043/100‑1 (MQ=255)
                                                                           ccAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTgg                    <  1:1109343/100‑1 (MQ=255)
                                                                           ccAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTgg                    <  1:2098390/100‑1 (MQ=255)
                                                                            cAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGc                   <  2:1015665/100‑1 (MQ=255)
                                                                            cAGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGc                   <  1:1388788/100‑1 (MQ=255)
                                                                             aGATCTCACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCg                  <  1:871706/100‑1 (MQ=255)
                                                                                tctcACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATg               >  2:1250137/1‑100 (MQ=255)
                                                                                 ctcACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGc              <  1:1941233/100‑1 (MQ=255)
                                                                                  tcACAATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCa             >  1:427028/1‑100 (MQ=255)
                                                                                    acaATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAgg           >  1:386258/1‑100 (MQ=255)
                                                                                    acaATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAgg           <  2:633032/100‑1 (MQ=255)
                                                                                     caATGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAggg          >  1:866221/1‑100 (MQ=255)
                                                                                       aTGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGcc        <  1:1781642/100‑1 (MQ=255)
                                                                                       aTGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGcc        >  1:1606219/1‑100 (MQ=255)
                                                                                        tGCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCATACCGCTGACGACCTGGCGATGCAGGGCCt       >  1:823673/1‑100 (MQ=255)
                                                                                         gCTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTAGCGATGCAGGGCCTc      >  1:2304081/1‑100 (MQ=255)
                                                                                         gCTTTTTCGCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCGGGCGATGCAGGGCCTc      <  2:1964988/100‑1 (MQ=255)
                                                                                          cTTTTTCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCa     <  1:2165055/100‑1 (MQ=255)
                                                                                             tttCTCCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATc  >  1:1705029/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CATTGTTAAATGTAAGTTAAAACACCATTGTCAGGGATATTCTTCTGTAAGGCAATTCCCGGCTTAGTCACCGGCCCAGATCTCACAATGCTTTTTCACCAGCCCAATCAGCGAGCCGCCATCGGCGACGAAATCGCGCATTAGCTGCGCTTCATTCAGACCGCTGACGACCTGGCGATGCAGGGCCTCAATC  >  NC_000913/606202‑606394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: