| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 36,231 | T→C | intergenic (‑69/+40) | caiD ← / ← caiC | carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 36,231 | 0 | T | C | 96.4% | 280.4 / 0.2 | 83 | intergenic (‑69/+40) | caiD/caiC | carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (48/32); total (48/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
GCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT > NC_000913/36136‑36327 | gCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTTTTTTTTTTTTTACGACAACATCCCTGCAAAAAATGTATATTGTTTTAGAGCGTGa > 2:1078439/1‑100 (MQ=255) ggTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGatta < 2:610418/100‑1 (MQ=255) ccgatcttGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGatta < 2:303022/93‑1 (MQ=255) tCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGattatt > 1:1904343/1‑100 (MQ=255) gacaagcaaTGAGTGAATCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAATTGCATATTTGTTTAGAGCGTGAttatta > 2:2183553/8‑100 (MQ=255) aGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGAAAAAAAAAAAAAAAAAAATGCATATTGTTTTAGAGCGTGATTATTAg < 2:581261/100‑1 (MQ=255) gATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGc < 1:30034/100‑1 (MQ=255) tGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGattaagatcgg > 1:303024/1‑93 (MQ=255) tGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTg > 2:67989/1‑100 (MQ=255) gTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTgg > 1:783528/1‑100 (MQ=255) tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc < 2:926933/100‑1 (MQ=255) tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc > 2:197192/1‑100 (MQ=255) tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc > 1:1502434/1‑100 (MQ=255) aTGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAg < 1:401180/100‑1 (MQ=255) tGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAgg < 2:1923285/100‑1 (MQ=255) tGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAgg < 2:1571119/100‑1 (MQ=255) aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt < 1:2171736/100‑1 (MQ=255) tCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAg < 1:1552509/100‑1 (MQ=255) cACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGt > 1:815914/1‑100 (MQ=255) cACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGt > 2:666325/1‑100 (MQ=255) aCTCATTCCTTTTCTCCATTTTTTTTTTTTTTTTTACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGtt > 2:1355589/1‑100 (MQ=255) cATTCCTTTTCTCCATTTTTTCTTTTTTTTTTACGACAACATCCCTGCAAAAAATTCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGtt > 2:351863/1‑97 (MQ=255) aTTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCct > 2:1842123/1‑100 (MQ=255) aTTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCct > 2:43314/1‑100 (MQ=255) cTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCctgctg > 2:1555868/1‑100 (MQ=255) ttttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGt < 1:43316/100‑1 (MQ=255) tttCTCCATTTTTTCTTTTTTATGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTACTGtt > 2:400386/1‑100 (MQ=255) tttCTCCATTTTTGCTTTTTCAGGGGCGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGtt < 1:2071041/100‑1 (MQ=255) tcCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCat > 1:658155/1‑100 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc < 1:351865/100‑1 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc > 2:721200/1‑100 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc > 2:623178/1‑100 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc < 2:644679/100‑1 (MQ=255) ttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTc < 1:1859156/100‑1 (MQ=255) tGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCa > 1:2170195/1‑100 (MQ=255) tGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCa > 1:2106140/1‑100 (MQ=255) gCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAg < 2:2237995/100‑1 (MQ=255) gCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAg > 1:866171/1‑100 (MQ=255) tttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAt > 1:1594945/1‑100 (MQ=255) tttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAt > 1:2150393/1‑100 (MQ=255) ttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttct > 2:1936455/1‑100 (MQ=255) ttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttct > 1:1558468/1‑100 (MQ=255) cAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttcttt > 2:502607/1‑100 (MQ=255) aGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttc < 1:1559461/100‑1 (MQ=255) aGGGACGACAACATCCCTACAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttc > 2:2238339/1‑100 (MQ=255) gggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttct > 2:21046/1‑100 (MQ=255) ggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTa < 1:991331/100‑1 (MQ=255) gacgacAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTaa > 1:1346278/1‑100 (MQ=255) acgacAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAat > 2:1584517/1‑100 (MQ=255) acgacAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAat < 1:1864771/100‑1 (MQ=255) cgacAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAatt > 1:1947709/1‑100 (MQ=255) acaacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattat > 1:1205326/1‑100 (MQ=255) acaacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattat > 1:1949939/1‑100 (MQ=255) acaacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattat > 1:1985724/1‑100 (MQ=255) acaacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattat > 1:2302716/1‑100 (MQ=255) caacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattatt > 2:1679769/1‑100 (MQ=255) caTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTc < 1:1194224/100‑1 (MQ=255) aTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTcc > 1:1828919/1‑100 (MQ=255) aTCCCTGCAAAAAATGCATATTGTTTTAGAGCCTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTcc > 1:2213362/1‑100 (MQ=255) tCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc < 2:1664646/100‑1 (MQ=255) tCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc < 1:623179/100‑1 (MQ=255) cccTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTcccc < 2:2316204/100‑1 (MQ=255) ccTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCg < 2:1738156/100‑1 (MQ=255) tGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAg < 2:1985717/100‑1 (MQ=255) gCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTACCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGc > 2:1066226/1‑100 (MQ=255) cAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCa < 1:282535/100‑1 (MQ=255) aaaaaaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCa > 1:2076712/1‑99 (MQ=255) aaaaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAAtt < 2:1346275/100‑1 (MQ=255) aaaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTa < 1:197194/100‑1 (MQ=255) aaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTAc > 1:605080/1‑100 (MQ=255) gCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTg < 2:2316719/100‑1 (MQ=255) gCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTg < 2:827210/100‑1 (MQ=255) gCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTg > 2:1857006/1‑100 (MQ=255) atatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCTCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGc > 1:2174709/1‑100 (MQ=255) tatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCa > 1:2240961/1‑100 (MQ=255) tatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCa > 2:1811919/1‑100 (MQ=255) tatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCa < 1:881195/100‑1 (MQ=255) atTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAg < 2:1509473/100‑1 (MQ=255) gTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATc > 1:29057/1‑100 (MQ=255) tAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCtttt < 1:340381/100‑1 (MQ=255) agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc > 1:899347/1‑100 (MQ=255) agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTACTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc < 1:400388/100‑1 (MQ=255) gagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCt < 2:815912/100‑1 (MQ=255) | GCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT > NC_000913/36136‑36327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |