Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,079,672	G→A	R123H (CGC→CAC)	putP →	proline:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,079,672	0	G	A	97.9%	331.7 / ‑2.5	98	R123H (CGC→CAC)	putP	proline:sodium symporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (47/48);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTA  >  NC_000913/1079575‑1079767                                                                                                  |                                                                                                aaGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCcaca                                                                                               <  2:2317456/100‑1 (MQ=255)   gCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACAtt                                                                                             >  1:1262329/1‑100 (MQ=255)       gtggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTgc                                                                                         >  2:1344418/1‑100 (MQ=255)        tggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTgcg                                                                                        <  2:519755/100‑1 (MQ=255)        tggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTgcg                                                                                        <  2:2038038/100‑1 (MQ=255)             gggCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTa                                                                                   <  1:857492/100‑1 (MQ=255)             gggCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTa                                                                                   >  1:1356863/1‑100 (MQ=255)              ggCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTAt                                                                                  >  1:302979/1‑100 (MQ=255)                 ggTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTAtctc                                                                               <  2:76572/100‑1 (MQ=255)                 ggTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTAtctc                                                                               >  1:1492054/1‑100 (MQ=255)                  gTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATctct                                                                              >  1:2198321/1‑100 (MQ=255)                  gTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATctct                                                                              >  2:2015082/1‑100 (MQ=255)                   ttGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTg                                                                             <  1:2112757/100‑1 (MQ=255)                    tGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTgc                                                                            >  2:506926/1‑100 (MQ=255)                     gCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTgcg                                                                           <  2:2300306/100‑1 (MQ=255)                     gCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTgcg                                                                           >  2:1526810/1‑100 (MQ=255)                         gtgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTgg                                                                       <  1:2197693/100‑1 (MQ=255)                          tgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGt                                                                      >  1:1415546/1‑100 (MQ=255)                          tgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGt                                                                      >  1:1117162/1‑100 (MQ=255)                          tgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGt                                                                      >  2:949244/1‑100 (MQ=255)                           gCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGt                                                                      <  2:991120/99‑1 (MQ=255)                           gCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGACCCATTTTGCGCATTATCTCTGCGCTGGt                                                                      >  2:433943/1‑99 (MQ=255)                            cATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTa                                                                    >  2:619568/1‑100 (MQ=255)                            cATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTa                                                                    <  2:1356860/100‑1 (MQ=255)                            cATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTa                                                                    >  2:898091/1‑100 (MQ=255)                            cATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTa                                                                    >  2:1264131/1‑100 (MQ=255)                               cttccgatctacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAttt                                                                 <  2:2182438/90‑1 (MQ=255)                               aCCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAttt                                                                 <  2:1597885/100‑1 (MQ=255)                                ccGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAtttt                                                                <  2:302977/100‑1 (MQ=255)                                ccGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAtttt                                                                >  1:1694122/1‑100 (MQ=255)                                 cGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTg                                                               <  2:869110/100‑1 (MQ=255)                                 cGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTg                                                               <  1:686458/100‑1 (MQ=255)                                 cGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTg                                                               <  1:686430/100‑1 (MQ=255)                                  gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                              <  1:2015089/100‑1 (MQ=255)                                  gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                              <  1:1834187/100‑1 (MQ=255)                                  gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                              >  2:1927474/1‑100 (MQ=255)                                  gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                              <  1:619569/100‑1 (MQ=255)                                       caacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttc                                                         >  2:691340/1‑100 (MQ=255)                                       caacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttc                                                         >  2:1835968/1‑100 (MQ=255)                                        aacacTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATTAAAGCTACCTTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                        >  2:1545813/1‑100 (MQ=255)                                        aacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                        <  2:1238515/100‑1 (MQ=255)                                        aacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                        <  1:184715/100‑1 (MQ=255)                                        aacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                        <  2:1113129/100‑1 (MQ=255)                                         acaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAtttagatcggaag                                                       >  1:2182445/1‑90 (MQ=255)                                          caaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttcttc                                                      <  2:546467/100‑1 (MQ=255)                                          caaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttcttc                                                      <  2:1707092/100‑1 (MQ=255)                                           aaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCa                                                     <  1:2283075/100‑1 (MQ=255)                                              aaCGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCa                                                     <  2:1626319/97‑1 (MQ=255)                                                cGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCAtt                                                >  2:907439/1‑100 (MQ=255)                                                 gCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCAttt                                               <  1:1264134/100‑1 (MQ=255)                                                  ccTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTa                                              >  1:1765884/1‑100 (MQ=255)                                                     tAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTg                                           <  2:2102963/100‑1 (MQ=255)                                                     tAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTg                                           <  1:1319280/100‑1 (MQ=255)                                                      aaCACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgc                                          >  2:202693/1‑100 (MQ=255)                                                      aaCACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgc                                          <  1:2035331/100‑1 (MQ=255)                                                      aaCACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgc                                          <  2:1564158/100‑1 (MQ=255)                                                      aaCACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgc                                          <  1:1503499/100‑1 (MQ=255)                                                       acacTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcg                                         >  1:1114748/1‑100 (MQ=255)                                                        cacTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcgc                                        >  1:546366/1‑100 (MQ=255)                                                            gCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCg                                    >  1:2334481/1‑100 (MQ=255)                                                            gCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCg                                    >  1:2334582/1‑100 (MQ=255)                                                            gCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCg                                    >  1:1378044/1‑100 (MQ=255)                                                             ccGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACGTTTTGCGAATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCgg                                   <  1:1214717/100‑1 (MQ=255)                                                              cGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCggg                                  <  1:292232/100‑1 (MQ=255)                                                               ggATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGc                                 <  1:1344421/100‑1 (MQ=255)                                                                gATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCa                                >  1:1973327/1‑100 (MQ=255)                                                                 attattTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCAt                               >  2:2131649/1‑100 (MQ=255)                                                                    attTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgt                            >  2:455664/1‑100 (MQ=255)                                                                     tttCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                           <  1:1264803/100‑1 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         <  2:687227/100‑1 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         <  2:1101335/100‑1 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         >  2:1903921/1‑100 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         <  1:907441/100‑1 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         >  1:1085220/1‑100 (MQ=255)                                                                       tCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                         >  2:2171813/1‑100 (MQ=255)                                                                        ccccGGGCGCTTTGCAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                           <  1:433945/95‑1 (MQ=255)                                                                        cACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCa                        >  1:479297/1‑100 (MQ=255)                                                                         aCCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAg                       >  2:2282414/1‑100 (MQ=255)                                                                         aCCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAg                       >  1:1028134/1‑100 (MQ=255)                                                                          ccGGGCGCTTTGAAGATAAATGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAg                       >  2:132302/1‑99 (MQ=255)                                                                           cGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgc                     >  2:1264595/1‑100 (MQ=255)                                                                            gggCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcg                    <  2:1745512/100‑1 (MQ=255)                                                                            gggCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcg                    <  2:1973320/100‑1 (MQ=255)                                                                                 cTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGt               >  1:148091/1‑100 (MQ=255)                                                                                    tGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCGGGCGCGCGTCTg            <  2:1378041/100‑1 (MQ=255)                                                                                     gAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGt           >  1:2196585/1‑100 (MQ=255)                                                                                     gAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGt           >  2:1863445/1‑100 (MQ=255)                                                                                       aGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGttt         <  1:1794981/100‑1 (MQ=255)                                                                                       aGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGttt         <  2:1765879/100‑1 (MQ=255)                                                                                        gATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTg        >  1:10742/1‑100 (MQ=255)                                                                                        gATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTg        <  2:906982/100‑1 (MQ=255)                                                                                        gATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTg        >  1:1222816/1‑100 (MQ=255)                                                                                          tAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGaa      <  1:2282423/100‑1 (MQ=255)                                                                                            aaaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAg    >  2:475875/1‑100 (MQ=255)                                                                                            aaaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAg    >  2:2332704/1‑100 (MQ=255)                                                                                            aaaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAg    <  2:2334472/100‑1 (MQ=255)                                                                                            aaaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAg    <  2:2334573/100‑1 (MQ=255)                                                                                             aaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGt   >  1:1152786/1‑100 (MQ=255)                                                                                              aGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTa  <  2:1694117/100‑1 (MQ=255)                                                                                                  |                                                                                                AAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTA  >  NC_000913/1079575‑1079767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑