Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,179,100	T→C	L170L (TTG→CTG)	nagK →	N‑acetyl‑D‑glucosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,179,100	0	T	C	100.0%	262.4 / NA	72	L170L (TTG→CTG)	nagK	N‑acetyl‑D‑glucosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

GCTGATTTTCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGTTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATT  >  NC_000913/1179003‑1179196                                                                                                  |                                                                                                 gCTGATTTTCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTg                                                                                                <  1:433174/100‑1 (MQ=255) gCTGATTTTCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTg                                                                                                <  2:1610222/100‑1 (MQ=255)  cTGATTTTCAACGGCAACCCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTgg                                                                                               <  2:2332251/100‑1 (MQ=255)  cTGATTTTCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTgg                                                                                               <  2:563096/100‑1 (MQ=255)   ccgatctCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGa                                                                                              <  1:1980830/94‑1 (MQ=255)      ttttCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGAttt                                                                                           >  1:1741130/1‑100 (MQ=255)      ttttCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGAttt                                                                                           >  2:2265652/1‑100 (MQ=255)       tttCAACGGCAAACCGAGTACCGGTAAAAGCTACAGTACCGGCGAGGTTGGCCATATTCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTc                                                                                          <  1:1952668/100‑1 (MQ=255)        ttCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTcc                                                                                         >  1:1871628/1‑100 (MQ=255)         tCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTccc                                                                                        <  1:1854219/100‑1 (MQ=255)         tCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTccc                                                                                        >  1:747510/1‑100 (MQ=255)         tCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTccc                                                                                        >  2:225279/1‑100 (MQ=255)         tCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTccc                                                                                        <  2:823488/100‑1 (MQ=255)         tCAACGGCAAACCGATTACCGGTAAAACCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTccc                                                                                        <  2:885690/100‑1 (MQ=255)          cAACGGCAAACCGATTACCGGTAAAAGCTAGATGACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCAGGATTTCCCg                                                                                       >  2:1534368/1‑100 (MQ=255)          cAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGaagatcgg                                                                                       >  2:1980823/1‑93 (MQ=255)          cAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCg                                                                                       <  1:1031153/100‑1 (MQ=255)            aCGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGtt                                                                                     <  1:1119257/100‑1 (MQ=255)               gCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTAcg                                                                                  >  1:1173728/1‑100 (MQ=255)               gCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTAcg                                                                                  <  2:601099/100‑1 (MQ=255)                 aaaCCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTAcgcc                                                                                <  1:1649547/100‑1 (MQ=255)                   aCCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTAcgccgc                                                                              <  1:225281/100‑1 (MQ=255)                         tACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGc                                                                        >  1:1562011/1‑100 (MQ=255)                         tACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGc                                                                        <  2:1959691/100‑1 (MQ=255)                         tACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGc                                                                        <  1:93776/100‑1 (MQ=255)                          aCCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCt                                                                       >  1:1717651/1‑100 (MQ=255)                           ccGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtg                                                                      <  1:1311835/100‑1 (MQ=255)                           ccGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtg                                                                      >  1:698173/1‑100 (MQ=255)                           ccGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtg                                                                      <  2:1562007/100‑1 (MQ=255)                           ccGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtg                                                                      >  2:1311832/1‑100 (MQ=255)                           atcttAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtg                                                                      <  1:1987545/96‑1 (MQ=255)                             ggTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtgtg                                                                    <  1:1806890/100‑1 (MQ=255)                              gTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtgtgn                                                                   <  1:1613226/100‑2 (MQ=255)                               tAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCtgagat                                                                  >  2:1987538/1‑96 (MQ=255)                                 aaaGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCa                                                                >  2:1843456/1‑100 (MQ=255)                                  aaGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAg                                                               >  2:198273/1‑100 (MQ=255)                                    gCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCa                                                             >  2:265727/1‑100 (MQ=255)                                    gCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGACTGTGGTCAGCa                                                             <  1:1534372/100‑1 (MQ=255)                                          ttACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTg                                                       <  2:132073/100‑1 (MQ=255)                                           tACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGc                                                      >  2:165695/1‑100 (MQ=255)                                            aCCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCa                                                     >  1:2066755/1‑100 (MQ=255)                                             ccGGCGAGTTTGGCCATATTCGTCGGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGTTGTGGTCAGCATGGCTGCAt                                                    >  2:1511622/1‑100 (MQ=255)                                             ccGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGcct                                                    >  1:1594903/1‑98 (MQ=255)                                              cGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCAtt                                                   >  1:138021/1‑100 (MQ=255)                                              cGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCAtt                                                   <  2:174244/100‑1 (MQ=255)                                               ggCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTg                                                  >  1:717703/1‑100 (MQ=255)                                                   aGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGaaaa                                              >  1:282491/1‑100 (MQ=255)                                                    gTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGaaaa                                              >  1:1337023/1‑99 (MQ=255)                                                        ggCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTAtc                                         <  1:156507/100‑1 (MQ=255)                                                        ggCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTAtc                                         >  2:391485/1‑100 (MQ=255)                                                        ggCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTAtc                                         <  2:717702/100‑1 (MQ=255)                                                               tGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTgg                                  <  2:1741125/100‑1 (MQ=255)                                                                  gTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTcg                               <  1:165697/100‑1 (MQ=255)                                                                  gTCTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTcg                               <  2:1384110/100‑1 (MQ=255)                                                                    cTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGTTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTcgcg                             <  1:1511626/100‑1 (MQ=255)                                                                    cTGCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTcg                               <  2:213643/98‑1 (MQ=255)                                                                     tGCCGGTTGATGCGTTAACCATGCTGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTTGGCGCgg                            >  2:774726/1‑100 (MQ=255)                                                                      gCCGGTTGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGt                           >  1:2287495/1‑100 (MQ=255)                                                                            tGATGCGTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCg                     <  2:1796599/100‑1 (MQ=255)                                                                                 cGTTAACCATGATGGGGCTAGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCt                >  2:2253693/1‑100 (MQ=255)                                                                                  gTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTg               >  2:1876725/1‑100 (MQ=255)                                                                                  gTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTg               >  2:1674704/1‑100 (MQ=255)                                                                                  gTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTg               <  2:1173725/100‑1 (MQ=255)                                                                                  gTTAACCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTg               <  1:774727/100‑1 (MQ=255)                                                                                      aCCATGATGGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATc           <  2:1337020/100‑1 (MQ=255)                                                                                         atgatgGGGCTGGATTTCCCGTTACGCCGCTTCGGCTCTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAac        <  2:1579726/100‑1 (MQ=255)                                                                                            atgGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACt     >  1:2141915/1‑100 (MQ=255)                                                                                             tgGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACta    >  2:232559/1‑100 (MQ=255)                                                                                             tgGGGCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACta    <  2:1717646/100‑1 (MQ=255)                                                                                               gggCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACtatt  >  1:139946/1‑100 (MQ=255)                                                                                               gggCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACtatt  >  1:892506/1‑100 (MQ=255)                                                                                               gggCTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACtatt  >  1:321336/1‑100 (MQ=255)                                                                                                  |                                                                                                 GCTGATTTTCAACGGCAAACCGATTACCGGTAAAAGCTACATTACCGGCGAGTTTGGCCATATGCGTCTGCCGGTTGATGCGTTAACCATGATGGGGTTGGATTTCCCGTTACGCCGCTGCGGCTGTGGTCAGCATGGCTGCATTGAAAATTATCTGTCTGGTCGCGGTTTTGCGTGGCTGTATCAACACTATT  >  NC_000913/1179003‑1179196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑