Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,282,840 G→T E993* (GAG→TAG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,282,8400GT96.8% 328.9 / 2.1 95E993* (GAG→TAG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (44/48);  total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACA  >  NC_000913/1282744‑1282933
                                                                                                |                                                                                             
ccGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACtaga                                                                                            >  1:130803/1‑100 (MQ=255)
ccGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACtag                                                                                             >  1:1963107/1‑99 (MQ=255)
 cGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTagaa                                                                                           <  2:640231/100‑1 (MQ=255)
  ggAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGagaag                                                                                          <  1:2126382/100‑1 (MQ=255)
    aaaCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGAt                                                                                        >  1:688595/1‑100 (MQ=255)
      aCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATcc                                                                                      >  1:1808276/1‑100 (MQ=255)
       ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATc                                                                                       >  2:597998/1‑98 (MQ=255)
       ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCg                                                                                     >  1:207409/1‑100 (MQ=255)
       ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCg                                                                                     <  1:1807347/100‑1 (MQ=255)
       ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCg                                                                                     >  1:601762/1‑100 (MQ=255)
        cAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGc                                                                                    >  2:1276442/1‑100 (MQ=255)
        cAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGc                                                                                    >  2:2180912/1‑100 (MQ=255)
         atctgTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCt                                                                                   <  1:1790600/96‑1 (MQ=255)
         aaCGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCt                                                                                   <  2:601761/100‑1 (MQ=255)
         aaCGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCt                                                                                   <  2:381784/100‑1 (MQ=255)
            ggTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTcc                                                                                >  1:949036/1‑100 (MQ=255)
             gTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCtagat                                                                               >  2:1790595/1‑96 (MQ=255)
             gTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCcg                                                                               >  2:1968907/1‑100 (MQ=255)
              tCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCcgc                                                                              <  1:1143994/100‑1 (MQ=255)
               cAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCcgcg                                                                             <  2:290020/100‑1 (MQ=255)
               cAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCcgcg                                                                             <  1:790602/100‑1 (MQ=255)
                aGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGa                                                                            <  1:1370970/100‑1 (MQ=255)
                 ggTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGat                                                                           <  2:958056/100‑1 (MQ=255)
                  gTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGata                                                                          >  1:2013094/1‑100 (MQ=255)
                    aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATAtt                                                                        >  1:284655/1‑100 (MQ=255)
                     gCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTc                                                                       <  2:1653534/100‑1 (MQ=255)
                        gTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAgg                                                                    <  1:672556/100‑1 (MQ=255)
                             aGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAg                                                               >  1:1998716/1‑100 (MQ=255)
                               ccTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGc                                                              <  2:1808271/99‑1 (MQ=255)
                                cTGGGCGGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGAGCCGCTTCCGCGATATTCAGGCACAGCcg                                                            <  1:581814/100‑1 (MQ=255)
                                  gggCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                          >  2:1972380/1‑100 (MQ=255)
                                  gggCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                          <  2:284653/100‑1 (MQ=255)
                                  gggCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                          <  1:1792135/100‑1 (MQ=255)
                                  gggCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                          <  2:1336182/100‑1 (MQ=255)
                                  gggCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgcg                                                          <  1:1218138/100‑1 (MQ=255)
                                       gCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa                                                     >  2:2315119/1‑100 (MQ=255)
                                       gCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa                                                     >  1:324422/1‑100 (MQ=255)
                                        cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                                                    >  2:852360/1‑100 (MQ=255)
                                        cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                                                    <  1:1968914/100‑1 (MQ=255)
                                        cccTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                                                    <  1:1819481/100‑1 (MQ=255)
                                          cTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTa                                                  <  2:166231/100‑1 (MQ=255)
                                          cTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTa                                                  <  2:1454128/100‑1 (MQ=255)
                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTaac                                                >  1:2060450/1‑98 (MQ=255)
                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                                                >  1:1979117/1‑100 (MQ=255)
                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                                                >  1:1939136/1‑100 (MQ=255)
                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                                                >  1:1939121/1‑100 (MQ=255)
                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                                                <  2:130801/100‑1 (MQ=255)
                                             aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtct                                               >  2:629883/1‑100 (MQ=255)
                                              gCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtctc                                              >  1:645474/1‑100 (MQ=255)
                                               cGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTcc                                             >  2:642497/1‑100 (MQ=255)
                                               cGAATTTACCGGTCGCGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTcc                                             <  1:1396210/100‑1 (MQ=255)
                                                gAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCa                                            <  1:2253844/100‑1 (MQ=255)
                                                 aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATGAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAg                                           <  1:869731/100‑1 (MQ=255)
                                                 aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAg                                           >  2:1774348/1‑100 (MQ=255)
                                                  aTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGc                                          >  1:824566/1‑100 (MQ=255)
                                                   tttACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGcc                                         <  1:852362/100‑1 (MQ=255)
                                                     tACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg                                       >  1:170181/1‑100 (MQ=255)
                                                      aCCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa                                      <  2:1398519/100‑1 (MQ=255)
                                                      aCCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa                                      >  2:261696/1‑100 (MQ=255)
                                                       ccGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGAc                                     >  1:1026945/1‑100 (MQ=255)
                                                        cGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGAcc                                    <  1:752108/100‑1 (MQ=255)
                                                         ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGaccaggtc                                   <  1:2039032/100‑6 (MQ=255)
                                                         ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct                                   >  1:1490667/1‑100 (MQ=255)
                                                         ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct                                   >  1:1856605/1‑100 (MQ=255)
                                                         ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct                                   >  1:675258/1‑100 (MQ=255)
                                                         ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct                                   >  2:2055899/1‑100 (MQ=255)
                                                           tCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg                                 <  1:2032742/100‑1 (MQ=255)
                                                           tCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg                                 <  1:2180919/100‑1 (MQ=255)
                                                            cGTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggt                                >  1:1321371/1‑100 (MQ=255)
                                                             gTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc                               <  2:1682830/100‑1 (MQ=255)
                                                             gTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc                               <  2:1998709/100‑1 (MQ=255)
                                                             gTGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc                               <  2:207407/100‑1 (MQ=255)
                                                              tGACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtct                              <  1:642498/100‑1 (MQ=255)
                                                               gACCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctg                             >  2:781033/1‑100 (MQ=255)
                                                                aCCATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctgg                            <  2:2312004/100‑1 (MQ=255)
                                                                  cATACGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCtggtctggtc                          <  2:324420/100‑1 (MQ=255)
                                                                      cGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGa                      <  2:1939129/100‑1 (MQ=255)
                                                                      cGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGa                      <  2:1939114/100‑1 (MQ=255)
                                                                      cGCATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAACTCCAGCCCGACCTGGTCTGGTCTGGa                      <  2:2060443/100‑1 (MQ=255)
                                                                       gCATCTGGCGCTGAATAAAGAAGACTAGAAGAGCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGaa                     <  2:1963100/100‑1 (MQ=255)
                                                                        cATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAg                    <  2:949034/100‑1 (MQ=255)
                                                                        cATCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAg                    <  2:89397/100‑1 (MQ=255)
                                                                          tCTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGAt                  >  2:2022459/1‑100 (MQ=255)
                                                                           cTGGCGCTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATg                 >  2:1890793/1‑100 (MQ=255)
                                                                           cTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCCAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGa                   <  2:1255029/98‑1 (MQ=255)
                                                                              gcgcTGAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAac              <  1:1276445/100‑1 (MQ=255)
                                                                                   gAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGtt         <  2:170179/100‑1 (MQ=255)
                                                                                   gAATAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGtt         >  2:890510/1‑100 (MQ=255)
                                                                                     aTAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTc       >  2:1156433/1‑100 (MQ=255)
                                                                                     aTAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTc       >  1:1546966/1‑100 (MQ=255)
                                                                                      tAAAGAAGACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt      <  1:1614210/100‑1 (MQ=255)
                                                                                        aagaagACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa    >  2:964339/1‑100 (MQ=255)
                                                                                        aagaagACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa    >  2:905395/1‑100 (MQ=255)
                                                                                        aagaagACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa    >  2:29824/1‑100 (MQ=255)
                                                                                        aagaagACTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa    >  1:13463/1‑100 (MQ=255)
                                                                                         agaagaCTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTAc   <  1:2315128/100‑1 (MQ=255)
                                                                                          gaagaCTAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa  >  1:1111330/1‑100 (MQ=255)
                                                                                                |                                                                                             
CCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACA  >  NC_000913/1282744‑1282933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: