Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,401,844 A→G I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,401,8440AG93.5% 295.2 / 3.9 95I471T (ATC→ACC) abgBp‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (48/39);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGT  >  NC_000913/1401747‑1401938
                                                                                                 |                                                                                              
ggaCGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTa                                                                                              <  2:948417/100‑1 (MQ=255)
 gacgacGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAg                                                                                             <  2:2199765/100‑1 (MQ=255)
  acgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAgg                                                                                            >  1:1762160/1‑100 (MQ=255)
   cgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAggg                                                                                           >  2:739706/1‑100 (MQ=255)
     acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCa                                                                                         >  1:1478956/1‑100 (MQ=255)
     acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCa                                                                                         <  2:1478952/100‑1 (MQ=255)
       ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGt                                                                                       >  2:1729246/1‑100 (MQ=255)
        gTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTg                                                                                      <  2:1762155/100‑1 (MQ=255)
        gTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTg                                                                                      <  2:461433/100‑1 (MQ=255)
          aTGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGAt                                                                                    <  2:237870/100‑1 (MQ=255)
           tGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATa                                                                                   >  2:841814/1‑100 (MQ=255)
           nggATGACATACTCATGGGCATTCCTCGGTAGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGATGACGGTGTCACGTTTTAAGGGGTAGGGCAGTGATa                                                                                   >  1:2003451/2‑100 (MQ=255)
            ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATAc                                                                                  <  1:1092011/100‑1 (MQ=255)
            ggATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATAc                                                                                  >  2:1564734/1‑100 (MQ=255)
               tGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGt                                                                               >  1:765865/1‑100 (MQ=255)
               ctaCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGt                                                                               <  2:1151327/98‑1 (MQ=255)
                gACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGtt                                                                              >  2:897990/1‑100 (MQ=255)
                 aCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGtag                                                                             >  1:1151329/1‑98 (MQ=255)
                 aCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTg                                                                             <  2:402525/100‑1 (MQ=255)
                 aCATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGCTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTg                                                                             >  2:2335489/1‑100 (MQ=255)
                   aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCg                                                                           >  1:342274/1‑100 (MQ=255)
                    tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                          >  2:976070/1‑100 (MQ=255)
                     aCTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCgtg                                                                         <  2:2070590/100‑1 (MQ=255)
                      cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                        >  1:1740352/1‑100 (MQ=255)
                      cTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgtgt                                                                        >  2:185861/1‑100 (MQ=255)
                        cATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTcc                                                                      <  2:972450/100‑1 (MQ=255)
                          tGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGt                                                                    >  2:1294682/1‑100 (MQ=255)
                            ggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTa                                                                  >  1:869257/1‑100 (MQ=255)
                             gCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTAc                                                                 <  2:342272/100‑1 (MQ=255)
                              cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                >  2:1788866/1‑100 (MQ=255)
                                 tCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGc                                                             <  2:294360/100‑1 (MQ=255)
                                 tCCTCGGTTGTTGTGTTTGCGTTGTTGTTCTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTCGGGCAGTGATACGGTTGCGTGTCCGTTACTTGc                                                             <  2:1409045/100‑1 (MQ=255)
                                  ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                            <  1:77744/100‑1 (MQ=255)
                                  ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                            >  2:278018/1‑100 (MQ=255)
                                  ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                            <  2:1030980/100‑1 (MQ=255)
                                  ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                            <  1:564119/100‑1 (MQ=255)
                                   cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                           >  2:1007297/1‑100 (MQ=255)
                                   cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                           >  1:598710/1‑100 (MQ=255)
                                    tCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGa                                                          <  2:1691560/100‑1 (MQ=255)
                                          gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctct                                                    >  2:1850401/1‑100 (MQ=255)
                                           ttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCtt                                                   <  2:538284/100‑1 (MQ=255)
                                             gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgt                                                 <  1:185863/100‑1 (MQ=255)
                                             gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgt                                                 >  2:228791/1‑100 (MQ=255)
                                              tgtTTGCGTTGTTGTTGTTATTTTTTTTTTTTTTTTTTCACGTTTTTCGGGTTAGGGCAGTGATACGGTTGCGTGTCTGTTACTTGTTGATGCTCttgtt                                                >  2:204018/1‑100 (MQ=255)
                                               gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                               >  2:1885181/1‑100 (MQ=255)
                                               gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                               <  2:1758377/100‑1 (MQ=255)
                                               gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                               <  1:2327915/100‑1 (MQ=255)
                                                tttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTGGTTgg                                              <  2:1049212/100‑1 (MQ=255)
                                                 ttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGc                                             <  2:751716/100‑1 (MQ=255)
                                                  tGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGc                                             <  1:976072/99‑1 (MQ=255)
                                                      ttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCt                                        >  2:2332234/1‑100 (MQ=255)
                                                      ttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCt                                        <  1:1226266/100‑1 (MQ=255)
                                                       tgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCCGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCtt                                       <  1:2014992/100‑1 (MQ=255)
                                                        gttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                      <  2:1310549/100‑1 (MQ=255)
                                                        gttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                      <  2:1700594/100‑1 (MQ=255)
                                                        gttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTg                                      <  1:2214906/100‑1 (MQ=255)
                                                         ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                     <  2:1377709/100‑1 (MQ=255)
                                                         ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                     <  1:739707/100‑1 (MQ=255)
                                                         ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                     <  1:841816/100‑1 (MQ=255)
                                                         ttgttgttAGTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                     <  1:1729251/100‑1 (MQ=255)
                                                         gatctgttAGTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                     <  1:1947583/96‑1 (MQ=255)
                                                           gttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                   >  1:2143717/1‑100 (MQ=255)
                                                           gttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                   >  1:254289/1‑100 (MQ=255)
                                                            ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt                                  >  2:902121/1‑100 (MQ=255)
                                                             tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                 >  1:683940/1‑100 (MQ=255)
                                                              gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAg                                >  1:383837/1‑100 (MQ=255)
                                                              gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAg                                >  1:1993585/1‑100 (MQ=255)
                                                              gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCagat                                 >  2:1947576/1‑96 (MQ=255)
                                                               ttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGc                               >  2:543327/1‑100 (MQ=255)
                                                                tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGTTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTAATGCTCTTGTTGGCATTCTTGCAATAGcc                              >  2:1410504/1‑100 (MQ=255)
                                                                tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGTCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                              >  2:2196700/1‑100 (MQ=255)
                                                                   tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                           >  1:1066308/1‑100 (MQ=255)
                                                                   tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                           >  1:225254/1‑100 (MQ=255)
                                                                    ttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGa                          >  1:2074932/1‑100 (MQ=255)
                                                                     tAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGaa                         >  2:1826245/1‑100 (MQ=255)
                                                                        aGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATATCCCTGAATCa                      >  2:775117/1‑100 (MQ=255)
                                                                         ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCaa                     >  1:102727/1‑100 (MQ=255)
                                                                         ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCaa                     <  2:2192459/100‑1 (MQ=255)
                                                                          gTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAg                    >  1:2029589/1‑100 (MQ=255)
                                                                           tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                   <  2:388040/100‑1 (MQ=255)
                                                                            gACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGa                  >  2:1298653/1‑100 (MQ=255)
                                                                              cGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAag                >  1:1112766/1‑100 (MQ=255)
                                                                              cGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAag                <  2:1326258/100‑1 (MQ=255)
                                                                              cGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAag                >  1:740708/1‑100 (MQ=255)
                                                                                  gtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt            <  2:765864/100‑1 (MQ=255)
                                                                                    cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg          >  2:1546591/1‑100 (MQ=255)
                                                                                    cACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg          >  2:674353/1‑100 (MQ=255)
                                                                                    cACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAttn          <  1:755239/100‑2 (MQ=255)
                                                                                     aCGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTg          <  2:2197136/99‑1 (MQ=255)
                                                                                      cttttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc        <  2:1016818/98‑1 (MQ=255)
                                                                                      cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc        >  2:906865/1‑100 (MQ=255)
                                                                                       gTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACt       >  1:147205/1‑100 (MQ=255)
                                                                                         ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgt     >  2:1847354/1‑100 (MQ=255)
                                                                                         ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATCCTTGCCATAGCCCTGAATCAAGGAAGAGATTGACtgt     >  2:1573496/1‑100 (MQ=255)
                                                                                            tCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGt  <  2:598709/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGT  >  NC_000913/1401747‑1401938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑