Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,460,438	T→C	L462S (TTG→TCG)	paaH →	3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,460,438	0	T	C	97.4%	269.4 / ‑2.1	77	L462S (TTG→TCG)	paaH	3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (34/41);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAA  >  NC_000913/1460341‑1460530                                                                                                  |                                                                                             cTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgc                                                                                            <  1:397376/100‑1 (MQ=255)   tGCCTGGGTAGCGCAACTTGGCTGGCAGCGAATATTAAGGGTCCTTGAAAATCTACAGCATCGCTATGGCGAAGAACGCTATCGCCCATGTTCATcgct                                                                                           <  1:2025969/99‑1 (MQ=255)   tGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgctg                                                                                          >  1:1140136/1‑100 (MQ=255)   tGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgctg                                                                                          >  1:666554/1‑100 (MQ=255)   tGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgctg                                                                                          <  1:297240/100‑1 (MQ=255)      cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcgc                                                                                       >  2:377680/1‑100 (MQ=255)       tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                      <  1:377682/100‑1 (MQ=255)       tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                      <  2:1595700/100‑1 (MQ=255)         gggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCaa                                                                                    >  1:441743/1‑100 (MQ=255)            aGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACgg                                                                                 >  2:1269543/1‑100 (MQ=255)             gcgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACggg                                                                                <  1:1093034/100‑1 (MQ=255)              cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgc                                                                               <  2:208220/100‑1 (MQ=255)               gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgcg                                                                              <  1:608228/100‑1 (MQ=255)                cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgcgc                                                                             >  2:734775/1‑100 (MQ=255)                     tggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTg                                                                        <  2:1736098/100‑1 (MQ=255)                       gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGga                                                                      <  2:1565346/100‑1 (MQ=255)                        ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGgag                                                                     >  2:1839682/1‑100 (MQ=255)                         tggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGgaga                                                                    <  2:1146072/100‑1 (MQ=255)                            cAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCg                                                                 >  1:2097816/1‑100 (MQ=255)                              gCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGt                                                               <  2:441741/100‑1 (MQ=255)                                gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTa                                                             <  1:2337313/100‑1 (MQ=255)                                gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTa                                                             <  1:351813/100‑1 (MQ=255)                                  atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                           <  1:25833/100‑1 (MQ=255)                                  atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                           <  1:1390513/100‑1 (MQ=255)                                  atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                           <  2:268415/100‑1 (MQ=255)                                  atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                           <  2:666553/100‑1 (MQ=255)                                    atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                         <  1:1972700/100‑1 (MQ=255)                                    atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                         <  1:1141465/100‑1 (MQ=255)                                    atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                         >  2:1289384/1‑100 (MQ=255)                                    atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                         <  1:1846644/100‑1 (MQ=255)                                      tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                       >  2:2312729/1‑100 (MQ=255)                                      tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                       >  2:2176074/1‑100 (MQ=255)                                      tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                       >  1:598920/1‑100 (MQ=255)                                      tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                       >  1:442559/1‑100 (MQ=255)                                       aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                      <  2:931327/100‑1 (MQ=255)                                         ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATa                                                    >  2:1684704/1‑100 (MQ=255)                                         ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATa                                                    >  1:813030/1‑100 (MQ=255)                                         ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATa                                                    >  1:1124614/1‑100 (MQ=255)                                          gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATaa                                                   >  1:2094843/1‑100 (MQ=255)                                          gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATaa                                                   <  2:1755610/100‑1 (MQ=255)                                            tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAgg                                                 <  1:2012844/100‑1 (MQ=255)                                               ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCt                                              >  2:58923/1‑100 (MQ=255)                                               ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCt                                              >  2:985154/1‑100 (MQ=255)                                                 gAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                            <  1:327514/100‑1 (MQ=255)                                                  aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGc                                           <  1:1789540/100‑1 (MQ=255)                                                    aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaa                                         >  1:2075030/1‑100 (MQ=255)                                                         aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGc                                    <  2:2075023/100‑1 (MQ=255)                                                         aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGc                                    <  2:673612/100‑1 (MQ=255)                                                         aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGc                                    <  2:2332063/100‑1 (MQ=255)                                                            gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGccc                                  <  2:2338004/99‑1 (MQ=255)                                                            gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                 >  2:1477588/1‑100 (MQ=255)                                                            gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                 >  1:1914220/1‑100 (MQ=255)                                                            gCATCACTATGGCGAAGAACGCGATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                 >  2:1362106/1‑100 (MQ=255)                                                               gatcTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGc                              <  2:406210/97‑1 (MQ=255)                                                                cACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                             <  2:442557/100‑1 (MQ=255)                                                                 aCTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCaa                            >  1:2218844/1‑100 (MQ=255)                                                                 aCTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCaa                            >  2:986265/1‑100 (MQ=255)                                                                  cTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                           >  2:2168753/1‑100 (MQ=255)                                                                  cTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                           <  1:127424/100‑1 (MQ=255)                                                                  cTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                           <  2:38690/100‑1 (MQ=255)                                                                  cTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCGGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                           <  1:1362109/100‑1 (MQ=255)                                                                    aTGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCagatc                         >  1:406212/1‑96 (MQ=255)                                                                    aTGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                         <  1:422813/100‑1 (MQ=255)                                                                     tGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                        >  2:1673930/1‑100 (MQ=255)                                                                      ggCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAt                       <  2:1579852/100‑1 (MQ=255)                                                                       gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                      >  2:124721/1‑100 (MQ=255)                                                                       gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTAATAAGGCCTGGCAAAATGCCCATGCAATGTATg                      >  1:2088641/1‑100 (MQ=255)                                                                         gaagaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                    <  2:492382/100‑1 (MQ=255)                                                                            gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                 <  1:734776/100‑1 (MQ=255)                                                                               cGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGAt              >  2:2090063/1‑100 (MQ=255)                                                                                gCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg             <  1:58925/100‑1 (MQ=255)                                                                                  tATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc           <  1:1432845/100‑1 (MQ=255)                                                                                  tATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc           >  2:2279917/1‑100 (MQ=255)                                                                                  tATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc           <  1:1160464/100‑1 (MQ=255)                                                                                    tCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTg         <  1:2312738/100‑1 (MQ=255)                                                                                    tCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTg         <  1:2176081/100‑1 (MQ=255)                                                                                      gCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg       >  1:879225/1‑100 (MQ=255)                                                                                       cccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc      >  2:26714/1‑100 (MQ=255)                                                                                       cccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc      >  2:968878/1‑100 (MQ=255)                                                                                       cccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc      >  2:968901/1‑100 (MQ=255)                                                                                           tGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaaa  >  2:566545/1‑100 (MQ=255)                                                                                           tGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaaa  >  2:254523/1‑100 (MQ=255)                                                                                                  |                                                                                             CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAA  >  NC_000913/1460341‑1460530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑