| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,580,094 | C→T | D233N (GAT→AAT) | ydeM ← | putative YdeN‑specific sulfatase‑maturating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,580,094 | 0 | C | T | 97.8% | 310.6 / ‑2.0 | 90 | D233N (GAT→AAT) | ydeM | putative YdeN‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (40/48); total (41/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
CCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATG > NC_000913/1579998‑1580190 | ccTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtt > 2:735511/1‑100 (MQ=255) tGCAGGACCCCTGGAAAATACAACTGGTATGCCCATTCCCCAAACAACGGCTGACAAAGCTTTCAAACCGACGGATGAAAATTTCACCCACATTGtttt < 1:2226506/99‑1 (MQ=255) ggACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAAcc > 2:454091/1‑100 (MQ=255) ggACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAAcc > 1:475588/1‑100 (MQ=255) ggACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAAcc < 1:890042/100‑1 (MQ=255) aCTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCa < 2:1778674/100‑1 (MQ=255) cTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCAt > 1:2123883/1‑100 (MQ=255) tCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCAtt < 1:1778507/100‑1 (MQ=255) tCCTGGAAAATACAACTGGTATGACCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCa < 2:347294/98‑1 (MQ=255) ccTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTg > 1:641490/1‑100 (MQ=255) ccTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTg < 1:1680545/100‑1 (MQ=255) cTGGAAAATACAACGGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGc < 1:2087697/100‑1 (MQ=255) tctaaaaTACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCa < 2:706623/97‑1 (MQ=255) tcgAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCACACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCa < 1:1866293/98‑1 (MQ=255) tGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCa > 1:1050198/1‑100 (MQ=255) tGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCa > 1:1888957/1‑100 (MQ=255) ggAAAATACAACTGGTATGCCCATTCCCCAAAAGACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt > 2:1404212/1‑100 (MQ=255) ggAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt < 2:2074353/100‑1 (MQ=255) aaaaTACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCaaga > 1:706624/1‑97 (MQ=255) aaTACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATaaaa > 1:1898592/1‑100 (MQ=255) aTACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATaaaaa > 2:1031835/1‑100 (MQ=255) cAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATgg < 1:1442161/100‑1 (MQ=255) cAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATgg > 1:2327890/1‑100 (MQ=255) aaCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGt > 1:1680312/1‑100 (MQ=255) aCTGGTATTCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGtt < 2:2327881/100‑1 (MQ=255) aCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGtt > 1:243736/1‑100 (MQ=255) cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTg < 2:1968902/100‑1 (MQ=255) tGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGa < 1:735512/100‑1 (MQ=255) ggTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGa > 1:2243567/1‑99 (MQ=255) ggTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGAc > 1:765117/1‑100 (MQ=255) ggTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGAc < 1:1362445/100‑1 (MQ=255) gTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa < 1:1132578/100‑1 (MQ=255) atctGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa < 2:483336/97‑1 (MQ=255) atctGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa < 2:725923/97‑1 (MQ=255) tATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACAt > 1:255974/1‑100 (MQ=255) tGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACAtga > 2:77977/1‑98 (MQ=255) gCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACaagat > 1:483338/1‑96 (MQ=255) gCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACaagat > 1:725924/1‑96 (MQ=255) gCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACAtgaa > 2:1113715/1‑97 (MQ=255) cgatctCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAc < 2:1135812/95‑1 (MQ=255) ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACt > 1:1458447/1‑100 (MQ=255) ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc < 1:2205736/100‑1 (MQ=255) ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc < 2:201493/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 2:1506395/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 1:1718203/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 2:1989529/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 1:385651/100‑1 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAcagatcg > 1:1135814/1‑94 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:765389/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 2:2061935/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:1952436/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:67326/1‑100 (MQ=255) cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCAt < 1:454093/100‑1 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa > 2:933514/1‑100 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa > 2:523603/1‑100 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa < 2:1820260/100‑1 (MQ=255) aaaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAg > 1:1758488/1‑100 (MQ=255) aCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTg < 1:1113717/100‑1 (MQ=255) aCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTg < 1:77979/100‑1 (MQ=255) ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTggg < 1:1412444/100‑1 (MQ=255) gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 1:2061942/100‑1 (MQ=255) atctcAAAGCTTTCAAACTGACAGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 1:1957331/95‑1 (MQ=255) tGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAgg < 2:1717869/100‑1 (MQ=255) gACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGc < 1:325693/100‑1 (MQ=255) cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGaagatc > 2:1957324/1‑95 (MQ=255) cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGcac < 2:1680307/100‑1 (MQ=255) aaaGCTTTCAAACTGACGGATGAAAATTTCACTCACATTGGTTTTAACCCATTGCATAAAAATGGTTGACAGGAACTTGCCATAAGCCGTGGGAGGcaca < 2:1461670/100‑1 (MQ=255) cAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc < 2:243734/100‑1 (MQ=255) aaCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCaa > 2:878589/1‑100 (MQ=255) aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 2:730527/100‑1 (MQ=255) ggATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACg > 1:728695/1‑100 (MQ=255) gATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 2:1050196/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt > 1:449661/1‑100 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt < 1:523605/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt < 2:1378332/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt > 2:574653/1‑100 (MQ=255) aaTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtgtg > 1:698629/1‑100 (MQ=255) aTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGAAAAAAAAAAAAAAATCAATGATACGGAATgtgt < 2:641489/100‑1 (MQ=255) ttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtc < 1:2084953/100‑1 (MQ=255) tCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:574654/100‑1 (MQ=255) cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc > 2:2292136/1‑100 (MQ=255) cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 2:1884299/100‑1 (MQ=255) cACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 2:1898585/100‑1 (MQ=255) aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa < 2:700327/100‑1 (MQ=255) aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa > 2:1820809/1‑100 (MQ=255) cccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc < 2:1888950/100‑1 (MQ=255) ccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt < 2:2123876/100‑1 (MQ=255) cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa > 1:1748288/1‑100 (MQ=255) cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa > 1:1003865/1‑100 (MQ=255) caTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg > 1:47911/1‑100 (MQ=255) | CCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATG > NC_000913/1579998‑1580190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |