| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,580,128 | A→G | F221F (TTT→TTC) | ydeM ← | putative YdeN‑specific sulfatase‑maturating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,580,128 | 0 | A | G | 98.8% | 288.7 / ‑4.5 | 82 | F221F (TTT→TTC) | ydeM | putative YdeN‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (38/43); total (38/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTT > NC_000913/1580032‑1580222 | cgatctCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAc < 2:1135812/95‑1 (MQ=255) ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACt > 1:1458447/1‑100 (MQ=255) ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc < 1:2205736/100‑1 (MQ=255) ttCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGc < 2:201493/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 2:1506395/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 1:385651/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 1:1718203/100‑1 (MQ=255) tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGcc < 2:1989529/100‑1 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAAcagatcg > 1:1135814/1‑94 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:1952436/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 2:2061935/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:67326/1‑100 (MQ=255) ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCa > 1:765389/1‑100 (MQ=255) cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCAt < 1:454093/100‑1 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa > 2:933514/1‑100 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa < 2:1820260/100‑1 (MQ=255) ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATa > 2:523603/1‑100 (MQ=255) aaaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAg > 1:1758488/1‑100 (MQ=255) aCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTg < 1:77979/100‑1 (MQ=255) aCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTg < 1:1113717/100‑1 (MQ=255) ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTggg < 1:1412444/100‑1 (MQ=255) gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 1:2061942/100‑1 (MQ=255) atctcAAAGCTTTCAAACTGACAGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGa < 1:1957331/95‑1 (MQ=255) tGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAgg < 2:1717869/100‑1 (MQ=255) gACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGc < 1:325693/100‑1 (MQ=255) cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGaagatc > 2:1957324/1‑95 (MQ=255) cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGcac < 2:1680307/100‑1 (MQ=255) aaaGCTTTCAAACTGACGGATGAAAATTTCACTCACATTGGTTTTAACCCATTGCATAAAAATGGTTGACAGGAACTTGCCATAAGCCGTGGGAGGcaca < 2:1461670/100‑1 (MQ=255) cAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc < 2:243734/100‑1 (MQ=255) aaCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCaa > 2:878589/1‑100 (MQ=255) aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 2:730527/100‑1 (MQ=255) ggATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACg > 1:728695/1‑100 (MQ=255) gATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 2:1050196/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt > 2:574653/1‑100 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt < 2:1378332/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt < 1:523605/100‑1 (MQ=255) gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt > 1:449661/1‑100 (MQ=255) aaTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtgtg > 1:698629/1‑100 (MQ=255) aTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGAAAAAAAAAAAAAAATCAATGATACGGAATgtgt < 2:641489/100‑1 (MQ=255) ttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtc < 1:2084953/100‑1 (MQ=255) tCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:574654/100‑1 (MQ=255) cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc > 2:2292136/1‑100 (MQ=255) cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 2:1884299/100‑1 (MQ=255) cACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 2:1898585/100‑1 (MQ=255) aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa < 2:700327/100‑1 (MQ=255) aCCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCa > 2:1820809/1‑100 (MQ=255) cccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc < 2:1888950/100‑1 (MQ=255) ccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt < 2:2123876/100‑1 (MQ=255) cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa > 1:1003865/1‑100 (MQ=255) cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa > 1:1748288/1‑100 (MQ=255) cattgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg < 2:255972/96‑1 (MQ=255) cattgTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg < 2:765388/96‑1 (MQ=255) caTTGTTTTTAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg > 1:47911/1‑100 (MQ=255) ttttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACt > 1:1585867/1‑100 (MQ=255) tttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg > 2:220422/1‑100 (MQ=255) tttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg < 1:1031837/100‑1 (MQ=255) tttAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTg < 2:1748283/100‑1 (MQ=255) tAACCCATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGaa > 2:1873750/1‑100 (MQ=255) cccATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc > 1:203633/1‑100 (MQ=255) cccATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc < 2:67324/100‑1 (MQ=255) cccATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATc > 1:491263/1‑100 (MQ=255) ccATTGCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCa < 1:1865063/100‑1 (MQ=255) gCATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATAtt > 1:1401303/1‑100 (MQ=255) cATAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTc > 1:2009214/1‑100 (MQ=255) tAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCgg < 2:203631/100‑1 (MQ=255) tAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCgg < 1:125560/100‑1 (MQ=255) tAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCgg > 1:336739/1‑100 (MQ=255) tAAAAATGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCgg > 1:850268/1‑100 (MQ=255) aaaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGc > 1:676772/1‑100 (MQ=255) aaaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGc > 2:1719280/1‑100 (MQ=255) aaaaaTGGTTAACATGAACCCGCCATAAGCCGTAGGAGGCACAGAAAAAAAAATGATACGCAACGTGTTCTCACTATAACCTCTGAACGCAATAGTCAGc < 2:765116/100‑1 (MQ=255) aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg > 1:1917502/1‑100 (MQ=255) aaaaTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCg < 1:1873756/100‑1 (MQ=255) aTGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTcc > 1:622708/1‑100 (MQ=255) tGGTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTccc < 1:1404215/100‑1 (MQ=255) gTTGACATGAACTTGCCATAAGCCGTTGGAGGCACAGAAAAATCAATGATACGGAATGTTTTCTCACGATGACCACTTAAATCAATATTCGGCGTCCCgg < 2:2243559/100‑1 (MQ=255) gTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg > 1:405669/1‑100 (MQ=255) gTTGACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCgg > 1:405680/1‑100 (MQ=255) gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 2:978835/1‑100 (MQ=255) gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 1:2025155/1‑100 (MQ=255) gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt < 2:475586/100‑1 (MQ=255) gACATGAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGttt > 2:1134196/1‑100 (MQ=255) | CCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTT > NC_000913/1580032‑1580222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |