| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,686,092 | G→T | T166N (ACC→AAC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,686,092 | 0 | G | T | 97.3% | 256.8 / ‑0.9 | 74 | T166N (ACC→AAC) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (33/39); total (34/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTC > NC_000913/1685995‑1686187 | ncccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGttt > 1:2044852/2‑100 (MQ=255)ccccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGttt > 1:598583/1‑100 (MQ=255) cccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGtttt < 1:2252014/100‑1 (MQ=255) ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTtttt > 1:159597/1‑100 (MQ=255) ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTtttt > 1:1600605/1‑100 (MQ=255) cAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTa < 2:495278/100‑1 (MQ=255) cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGc > 1:1758183/1‑100 (MQ=255) cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGc < 1:2117248/100‑1 (MQ=255) cGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGACAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGc < 2:147090/100‑1 (MQ=255) gTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCt < 2:461253/100‑1 (MQ=255) tAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGa > 2:2124554/1‑100 (MQ=255) aCGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAgg < 1:2211554/100‑1 (MQ=255) aCGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAgg < 2:964657/100‑1 (MQ=255) cGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGa > 1:2059765/1‑100 (MQ=255) ggCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGaa < 2:1468735/100‑1 (MQ=255) gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGAGTCTGTGTGAGGTTTTTAAGCTGAGGAATGa > 2:1578837/1‑100 (MQ=255) gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGtt < 1:419090/100‑1 (MQ=255) cATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttg > 2:903036/1‑100 (MQ=255) aTCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgc < 1:44671/100‑1 (MQ=255) ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgctt > 1:2012710/1‑100 (MQ=255) ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcgg < 2:266787/100‑3 (MQ=255) cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttg < 1:928777/100‑1 (MQ=255) tGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttgc < 2:2002438/100‑1 (MQ=255) tGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGttgcttgc > 1:1147487/1‑100 (MQ=255) gCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcg < 2:1799947/100‑1 (MQ=255) cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc > 1:2140211/1‑100 (MQ=255) cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc > 1:2140115/1‑100 (MQ=255) cAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTgcgc < 2:809523/100‑1 (MQ=255) gTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAgc < 1:2100985/100‑1 (MQ=255) aGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGcc < 2:455155/100‑1 (MQ=255) gTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCca > 1:1423290/1‑100 (MQ=255) cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCcagca < 2:369077/100‑1 (MQ=255) gACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCTgctggcgctg < 1:1310094/100‑9 (MQ=255) aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc > 1:2019357/1‑100 (MQ=255) aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc > 1:2021258/1‑100 (MQ=255) aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCagcagc < 1:2209727/100‑1 (MQ=255) cAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAgcgc < 1:2145078/100‑1 (MQ=255) aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGcgcc > 2:2112887/1‑100 (MQ=255) ttGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCaa > 2:62552/1‑100 (MQ=255) gACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACg > 1:785077/1‑100 (MQ=255) cGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTg > 2:793103/1‑100 (MQ=255) tatCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAt > 1:677343/1‑100 (MQ=255) atCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAtn < 2:2044845/100‑2 (MQ=255) tCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCg > 1:868656/1‑100 (MQ=255) cGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGc > 1:1925515/1‑100 (MQ=255) aaaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTc < 1:784654/100‑1 (MQ=255) aaaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTc < 1:162198/100‑1 (MQ=255) aaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCg < 1:2264895/100‑1 (MQ=255) aaaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCg < 2:2012703/100‑1 (MQ=255) aaGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCgg > 2:1042954/1‑100 (MQ=255) aGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGa < 2:1005720/100‑1 (MQ=255) gCACGGGATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGaa < 1:903038/100‑1 (MQ=255) tccgatctctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGa < 1:1596045/93‑1 (MQ=255) gggATTTCTCATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCCCAGCGCCAGCTGCGCCGCAACGTGCATCGCCGTCGGAAAgcc < 1:1578842/100‑3 (MQ=255) gATTTCTCATTCAGTGTCTGTGCCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACAt > 2:1040568/1‑100 (MQ=255) tctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGtt > 2:1451686/1‑100 (MQ=255) ctcATTCAGTGTCTGTGTCAGGTTTTTTTTTTTTTTTTTTAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg > 2:1618037/1‑100 (MQ=255) ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGaagatcgga > 2:1596040/1‑92 (MQ=255) ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg > 1:1942577/1‑100 (MQ=255) ctcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTg < 2:1758178/100‑1 (MQ=255) tcATTCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTgg < 2:2059758/100‑1 (MQ=255) ttCAGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAAc < 1:793104/100‑1 (MQ=255) aGTGTCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACttt < 2:598582/100‑1 (MQ=255) gtgtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTg < 2:1147485/100‑1 (MQ=255) tgtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTgg < 2:1717903/100‑1 (MQ=255) gtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGc < 1:480668/100‑1 (MQ=255) gtCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCAGCGCCGTCGGAAAGACATCGTTGGAACTTTgg < 2:734788/99‑1 (MQ=255) tCTGTGTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt > 1:974136/1‑100 (MQ=255) tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt > 2:26206/1‑100 (MQ=255) tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt < 2:2140204/100‑1 (MQ=255) tgtgTCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCttt < 2:2140108/100‑1 (MQ=255) tCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGtt > 1:384516/1‑100 (MQ=255) tCAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGtt > 2:128533/1‑100 (MQ=255) cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:579538/1‑100 (MQ=255) cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:2237910/1‑100 (MQ=255) cAGGTTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc < 2:868654/100‑1 (MQ=255) aGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTc > 2:1539379/1‑99 (MQ=255) | CCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGGAATGAGTTGCTTGCGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCTTTTGTTC > NC_000913/1685995‑1686187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |