Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,955	G→A	A141V (GCC→GTC)	ruvC ←	component of RuvABC resolvasome, endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,955	0	G	A	100.0%	212.7 / NA	60	A141V (GCC→GTC)	ruvC	component of RuvABC resolvasome, endonuclease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/32);  total (28/32)

CGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTT  >  NC_000913/1946859‑1947050                                                                                                 |                                                                                                cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcgacgg                                                                                              <  2:661310/100‑1 (MQ=255)     gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATc                                                                                          <  1:1595155/100‑1 (MQ=255)       cGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCg                                                                                        >  1:234622/1‑100 (MQ=255)         cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGcc                                                                                      <  2:335277/100‑1 (MQ=255)         cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGcc                                                                                      <  1:1968208/100‑1 (MQ=255)          ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCt                                                                                     >  1:1706985/1‑100 (MQ=255)          ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCt                                                                                     >  1:733662/1‑100 (MQ=255)          ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCt                                                                                     >  1:1407349/1‑100 (MQ=255)            tctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCtgt                                                                                   <  2:677153/100‑1 (MQ=255)                    ggTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGc                                                                           <  1:1561506/100‑1 (MQ=255)                        cAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGc                                                                       <  1:1160237/100‑1 (MQ=255)                        cAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGc                                                                       <  1:1829787/100‑1 (MQ=255)                          gCCGCGATTCGCTCACCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAg                                                                     <  2:2107247/100‑1 (MQ=255)                               gATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCa                                                                <  2:744171/100‑1 (MQ=255)                                aTTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAg                                                               <  2:1491088/100‑1 (MQ=255)                                 ttCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGc                                                              >  2:1182911/1‑100 (MQ=255)                                     cTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAgg                                                          <  1:1182914/100‑1 (MQ=255)                                        aTCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGc                                                       <  1:2201741/100‑1 (MQ=255)                                         tCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATGGACGGCGTCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCg                                                      >  1:166699/1‑100 (MQ=255)                                            gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                   >  2:823459/1‑100 (MQ=255)                                              aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                 <  2:1181675/100‑1 (MQ=255)                                              aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                 >  1:911202/1‑100 (MQ=255)                                              aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                 <  1:663114/100‑1 (MQ=255)                                                tctaTTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                               <  1:712573/97‑1 (MQ=255)                                                cttccgatctcTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCTGGCAGTTTCAGCAAGGTGCGGACCata                                               <  1:1792304/90‑1 (MQ=255)                                                cGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                               >  1:1356490/1‑100 (MQ=255)                                                cGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                               <  1:1485032/100‑1 (MQ=255)                                                cGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                               >  1:1859371/1‑100 (MQ=255)                                                cGCATTCCGACCAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGTATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                               >  2:1228345/1‑100 (MQ=255)                                                   aTTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCataaga                                            >  2:712572/1‑97 (MQ=255)                                                      cTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtgctgc                                         >  1:1922417/1‑100 (MQ=255)                                                      cTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtgctgc                                         <  2:234620/100‑1 (MQ=255)                                                       tGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCa                                        <  2:2026449/100‑1 (MQ=255)                                                        gACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCa                                        <  1:2237808/99‑1 (MQ=255)                                                          cTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCTGGCAGTTTCAGCAAGGTGCGGACCataagatcggaag                                     >  2:1792299/1‑90 (MQ=255)                                                           tAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTg                                    <  2:1982298/100‑1 (MQ=255)                                                            aaCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTgg                                   >  2:351883/1‑100 (MQ=255)                                                            aaCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTgg                                   >  1:1884201/1‑100 (MQ=255)                                                                   cAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCtttttt                            <  1:351885/100‑1 (MQ=255)                                                                    aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                           >  2:930802/1‑100 (MQ=255)                                                                    aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                           <  2:1892132/100‑1 (MQ=255)                                                                    aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                           <  2:1845053/100‑1 (MQ=255)                                                                     gTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCg                          <  2:166697/100‑1 (MQ=255)                                                                      tGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCgg                         <  1:930804/100‑1 (MQ=255)                                                                         gTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTATCGGTCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGTCTTTTTTCGGCAc                      >  2:1599315/1‑100 (MQ=255)                                                                         gTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCAc                      >  1:361354/1‑100 (MQ=255)                                                                          tGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACt                     <  2:626962/100‑1 (MQ=255)                                                                          tGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACt                     <  2:733661/100‑1 (MQ=255)                                                                          tGATAGCAATCGCCAGCGCATCGACGGCACCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACt                     <  2:1407346/100‑1 (MQ=255)                                                                           gATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTg                    >  1:424568/1‑100 (MQ=255)                                                                            aTAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGc                   >  2:1875675/1‑100 (MQ=255)                                                                             tAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGcc                  <  1:576341/100‑1 (MQ=255)                                                                               gCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCaa                >  1:53873/1‑100 (MQ=255)                                                                                cAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAt               >  2:830266/1‑100 (MQ=255)                                                                                  aTCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtac             <  2:1922410/100‑1 (MQ=255)                                                                                  aTCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtac             <  1:1958553/100‑1 (MQ=255)                                                                                    cGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtacct           >  1:755646/1‑100 (MQ=255)                                                                                     gCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta          >  1:1658684/1‑100 (MQ=255)                                                                                        aGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTacca       >  2:688433/1‑100 (MQ=255)                                                                                         gcgcATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTaccac      >  2:1406028/1‑100 (MQ=255)                                                                                             aTCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGtt  >  2:2294944/1‑100 (MQ=255)                                                                                                 |                                                                                                CGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTT  >  NC_000913/1946859‑1947050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑