Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 194,506 A→T Q329L (CAG→CTG)  dxr → 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913194,5060AT98.8% 285.3 / ‑3.9 80Q329L (CAG→CTG) dxr1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (45/34);  total (46/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

ATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAT  >  NC_000913/194413‑194600
                                                                                             |                                                                                              
ccgatctcAAACTAAGTGCGTTCACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGGGGCGTTCGAACAAGGCCTGGCAGc                                                                                          <  2:1574971/93‑1 (MQ=255)
 tttttGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGGAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGcg                                                                                         >  2:1277207/1‑100 (MQ=255)
   tttGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGcgac                                                                                       >  1:1463790/1‑100 (MQ=255)
    ttGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGCTCGTTATCCATGCCTGAAACTGGCGCTGGAGGCGTTCGAACAAGGCCTGGCAGcgacg                                                                                      >  1:1143540/1‑100 (MQ=255)
      gCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCgacgac                                                                                    >  1:275021/1‑100 (MQ=255)
      gCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCgacgac                                                                                    >  2:100863/1‑100 (MQ=255)
       cgaaCTAAGTGCGATGACATTTGCCGCACCGCATTATGATCGCTATCCATGCCTGAAACTGGCGATGGGGGCGTTCGAACAAGGCCTGGCAGcagatcgg                                                                                   >  1:1574976/3‑93 (MQ=255)
        aaaCTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAg                                                                                  >  2:312477/1‑100 (MQ=255)
        aaaCTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAg                                                                                  <  2:2166848/100‑1 (MQ=255)
         aaCTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGc                                                                                 >  1:1564159/1‑100 (MQ=255)
            tAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCAtt                                                                              <  2:30695/100‑1 (MQ=255)
             aaGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTg                                                                             <  2:2336056/100‑1 (MQ=255)
              aGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGa                                                                            >  1:1004075/1‑100 (MQ=255)
                tGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAAt                                                                          <  2:841351/100‑1 (MQ=255)
                 gCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATg                                                                         >  1:1418825/1‑100 (MQ=255)
                 gCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATg                                                                         <  1:429162/100‑1 (MQ=255)
                  cGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGc                                                                        >  2:766267/1‑100 (MQ=255)
                  cGTTGACATTTGCCGCACCGGATTATGATCGTTATCAATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGc                                                                        >  1:1652767/1‑100 (MQ=255)
                   gTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGcc                                                                       >  1:2201351/1‑100 (MQ=255)
                   gTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGcc                                                                       >  1:1602941/1‑100 (MQ=255)
                    ttGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCg                                                                      <  2:516523/100‑1 (MQ=255)
                      gACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCa                                                                    >  1:102605/1‑100 (MQ=255)
                        cATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCaaa                                                                  <  2:1899011/100‑1 (MQ=255)
                          tttGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACg                                                                <  1:354549/100‑1 (MQ=255)
                          tttGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACg                                                                <  1:766268/100‑1 (MQ=255)
                           ttGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGa                                                               <  1:1552915/100‑1 (MQ=255)
                            tGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGaa                                                              >  2:502749/1‑100 (MQ=255)
                             gCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGaaa                                                             >  1:2043383/1‑100 (MQ=255)
                             gCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGaaa                                                             >  2:2243479/1‑100 (MQ=255)
                              ccGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAAt                                                            >  2:1345181/1‑100 (MQ=255)
                                   ccGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCg                                                       >  1:1064645/1‑100 (MQ=255)
                                       aTTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgc                                                   <  1:469750/100‑1 (MQ=255)
                                       aTTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgc                                                   <  2:2082842/100‑1 (MQ=255)
                                         tATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgctg                                                 <  2:2201343/100‑1 (MQ=255)
                                         tATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCACCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgctg                                                 >  2:1645579/1‑100 (MQ=255)
                                            gATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCtt                                              >  1:1346984/1‑100 (MQ=255)
                                            gATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCtt                                              >  1:2001136/1‑100 (MQ=255)
                                             ttccgatctcATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCttt                                             <  2:41468/91‑1 (MQ=255)
                                               cGTTATCCATGCCTGAAACTGGCGATGGAGGCGCTCGAACCAGACCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCttt                                             >  1:2140757/1‑98 (MQ=255)
                                                 ttATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCtt                                         >  2:2311396/1‑100 (MQ=255)
                                                  tATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTg                                        <  1:876904/100‑1 (MQ=255)
                                                     ccATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgcgc                                     <  1:1277210/100‑1 (MQ=255)
                                                      cATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCtttagatcggaa                                    >  1:41470/1‑91 (MQ=255)
                                                      cATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGca                                    <  2:2026467/100‑1 (MQ=255)
                                                      cATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGca                                    <  1:1791960/100‑1 (MQ=255)
                                                      cATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGca                                    <  1:239218/100‑1 (MQ=255)
                                                       aTGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaa                                   <  1:1873487/100‑1 (MQ=255)
                                                       aTGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaa                                   >  1:371103/1‑100 (MQ=255)
                                                         gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                 >  1:1449593/1‑100 (MQ=255)
                                                         gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                 >  2:167365/1‑100 (MQ=255)
                                                           cTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACaaa                               >  1:907486/1‑100 (MQ=255)
                                                           cTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACaaa                               >  1:585058/1‑100 (MQ=255)
                                                           cTGAAACAGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACaaa                               >  1:1229699/1‑100 (MQ=255)
                                                            tGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAAt                              <  2:508524/100‑1 (MQ=255)
                                                             gAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATc                             >  2:2222504/1‑100 (MQ=255)
                                                                 cTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCt                         >  2:1389432/1‑100 (MQ=255)
                                                                 cTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCt                         >  2:101462/1‑100 (MQ=255)
                                                                 cTGGCGATGGAGGCGTTCGAACAAGACCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCt                         >  2:661755/1‑100 (MQ=255)
                                                                  tGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCtt                        >  2:2207247/1‑100 (MQ=255)
                                                                   ggCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATGACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCttt                       >  1:2222252/1‑100 (MQ=255)
                                                                    gCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTa                      <  2:2269400/100‑1 (MQ=255)
                                                                     cGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTAc                     >  1:302751/1‑100 (MQ=255)
                                                                      gATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACg                    <  1:100865/100‑1 (MQ=255)
                                                                       aTGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                   <  1:1158212/100‑1 (MQ=255)
                                                                       aTGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                   <  2:1064643/100‑1 (MQ=255)
                                                                       aTGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                   <  2:1092764/100‑1 (MQ=255)
                                                                         ggAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATAGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGat                 >  2:1437337/1‑100 (MQ=255)
                                                                           aGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGatat               >  1:781132/1‑100 (MQ=255)
                                                                              cGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGc            >  2:2018841/1‑100 (MQ=255)
                                                                              cGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAAGGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGTATATCGc            >  2:1258340/1‑100 (MQ=255)
                                                                                ttCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTg          >  1:2184733/1‑100 (MQ=255)
                                                                                   gAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGt       <  2:2043376/100‑1 (MQ=255)
                                                                                    aaCAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGtt      >  2:1282880/1‑100 (MQ=255)
                                                                                     aCAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTg     <  2:1564155/100‑1 (MQ=255)
                                                                                     aCAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTg     <  1:1191132/100‑1 (MQ=255)
                                                                                      cAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGa    <  1:81604/100‑1 (MQ=255)
                                                                                      cAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGa    <  1:1778670/100‑1 (MQ=255)
                                                                                        aGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAt  <  1:502751/100‑1 (MQ=255)
                                                                                        aGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAt  <  1:2164427/100‑1 (MQ=255)
                                                                                        aGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAt  >  1:1290959/1‑100 (MQ=255)
                                                                                             |                                                                                              
ATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAT  >  NC_000913/194413‑194600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑