Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,191,432 C→T G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,191,4320CT97.6% 286.8 / ‑2.4 83G72S (GGC→AGC) yehCputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (37/44);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCG  >  NC_000913/2191335‑2191527
                                                                                                 |                                                                                               
aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtat                                                                                               <  1:1676640/100‑1 (MQ=255)
  ttttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatca                                                                                             <  1:1654182/100‑1 (MQ=255)
   tttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtatcat                                                                                            <  1:563307/100‑1 (MQ=255)
     tACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTAtcatca                                                                                          <  1:1173531/100‑1 (MQ=255)
      aCTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCaa                                                                                         <  1:526754/100‑1 (MQ=255)
      aCTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCaa                                                                                         >  1:1980071/1‑100 (MQ=255)
        ttGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATc                                                                                       <  1:351268/100‑1 (MQ=255)
         tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                      <  1:1094771/100‑1 (MQ=255)
         tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                      <  1:539413/100‑1 (MQ=255)
         tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                      <  1:2133870/100‑1 (MQ=255)
         tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                      <  1:2132154/100‑1 (MQ=255)
         tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                      >  1:1764148/1‑100 (MQ=255)
            tgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACg                                                                                   >  1:876669/1‑100 (MQ=255)
            tgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACg                                                                                   >  1:373329/1‑100 (MQ=255)
            tgcCCGGAATTTGCCCCTATTGTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACg                                                                                   <  2:1774163/100‑1 (MQ=255)
             gcCCGGAATTTGCCCCTATTTTTGTTTTTTTTTTCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                  >  2:1655320/1‑100 (MQ=255)
             gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                  >  2:581327/1‑100 (MQ=255)
              cccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGcc                                                                                 <  2:1005206/100‑1 (MQ=255)
              cccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGcc                                                                                 <  2:1764143/100‑1 (MQ=255)
                   aaTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgcag                                                                            <  2:1866381/100‑1 (MQ=255)
                    aTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgcagc                                                                           <  1:985360/100‑1 (MQ=255)
                    aTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgcagc                                                                           >  1:837921/1‑100 (MQ=255)
                       tGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCagcagc                                                                        >  1:897483/1‑100 (MQ=255)
                        gCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCg                                                                       <  1:1343110/100‑1 (MQ=255)
                           ccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaag                                                                    <  2:595090/100‑1 (MQ=255)
                           ccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCgaag                                                                    >  1:2162478/1‑100 (MQ=255)
                              aTTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAAc                                                                 <  1:548872/100‑1 (MQ=255)
                                ttttGCCCCTGGTGGCGTTAACATGCAAGGAACCTGACTTTTTTCTGGTGGTAATGACCTATCGCTATCATCCCTCCACGCCTGCAGCAGCGAAGAACgg                                                               <  1:583627/100‑1 (MQ=255)
                                ttttGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACgg                                                               >  1:1896915/1‑100 (MQ=255)
                                 tttGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGt                                                              <  1:920395/100‑1 (MQ=255)
                                  ttGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTTTTTTTTTTATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGtt                                                             >  2:795868/1‑100 (MQ=255)
                                    gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc                                                           >  1:1189727/1‑100 (MQ=255)
                                    gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc                                                           >  1:2274330/1‑100 (MQ=255)
                                    gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc                                                           >  1:2273492/1‑100 (MQ=255)
                                    gCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTc                                                           >  2:1687547/1‑100 (MQ=255)
                                     ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                          >  2:838558/1‑100 (MQ=255)
                                       aCTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCt                                                        <  2:1426671/100‑1 (MQ=255)
                                       aCTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCt                                                        <  2:1426679/100‑1 (MQ=255)
                                        cTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTg                                                       <  2:214533/100‑1 (MQ=255)
                                        cTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTg                                                       <  1:903744/100‑1 (MQ=255)
                                         tggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTgg                                                      <  2:1299405/100‑1 (MQ=255)
                                          ngtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGt                                                     >  1:584711/2‑100 (MQ=255)
                                          ggtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGt                                                     <  1:1178316/100‑1 (MQ=255)
                                           gtggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGtt                                                    <  1:523006/100‑1 (MQ=255)
                                            tggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                   >  2:2128293/1‑100 (MQ=255)
                                             ggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                  >  1:841944/1‑100 (MQ=255)
                                             ggCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                  >  1:934888/1‑100 (MQ=255)
                                                gTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatca                                               >  1:324132/1‑100 (MQ=255)
                                                  tAACATGAAAGGAACCTGAATTTTTTCTTTTTTTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                             >  2:2233061/1‑100 (MQ=255)
                                                  tAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                             <  2:75934/100‑1 (MQ=255)
                                                  tAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                             >  2:1017110/1‑100 (MQ=255)
                                                  cgatctGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                             <  1:480557/95‑1 (MQ=255)
                                                   aaCATGAAAGGAACCTGAATTTTTTTTTTTTTTTATGACGTATCGCTATCATCAATCCACGCCTTCAGCAGCGAAGAACGGTTTGCCTTGTTCATCAACt                                            >  2:925425/1‑100 (MQ=255)
                                                     cATGAAAGGAACCTGAATTTTTTTTTTTTTTTTTGACGTATAGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGTTTCATCAACTGc                                          >  2:1184648/1‑100 (MQ=255)
                                                     cATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGc                                          <  1:291152/100‑1 (MQ=255)
                                                       tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc                                        >  1:119138/1‑100 (MQ=255)
                                                       tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc                                        >  2:774913/1‑100 (MQ=255)
                                                       tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc                                        >  2:2157680/1‑100 (MQ=255)
                                                       tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc                                        >  1:1700725/1‑100 (MQ=255)
                                                        gAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAcagatcg                                       >  2:480555/1‑94 (MQ=255)
                                                        gAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAcc                                       <  1:2050817/100‑1 (MQ=255)
                                                         aaaGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCa                                      >  1:107700/1‑100 (MQ=255)
                                                         aaaGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCa                                      <  1:1626687/100‑1 (MQ=255)
                                                           aGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATc                                    <  2:819966/100‑1 (MQ=255)
                                                            ggAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCa                                   <  2:2273484/100‑1 (MQ=255)
                                                            ggAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCa                                   <  2:2274322/100‑1 (MQ=255)
                                                             gAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAc                                  <  1:1212713/100‑1 (MQ=255)
                                                                ccTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtc                               <  1:1687552/100‑1 (MQ=255)
                                                                  tGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtctt                             <  1:581328/100‑1 (MQ=255)
                                                                  tGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtctt                             >  1:835082/1‑100 (MQ=255)
                                                                   gAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCttt                            <  2:2103302/100‑1 (MQ=255)
                                                                       tttttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttn                        <  2:584710/100‑2 (MQ=255)
                                                                         tttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTc                      <  2:1700720/100‑1 (MQ=255)
                                                                             tggtggTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc                  >  1:987184/1‑100 (MQ=255)
                                                                               gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg                <  1:2237292/100‑1 (MQ=255)
                                                                               gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg                <  1:2157687/100‑1 (MQ=255)
                                                                               gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg                >  1:62800/1‑100 (MQ=255)
                                                                               gtggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCgg                <  1:774914/100‑1 (MQ=255)
                                                                                tggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCggg               >  2:1757287/1‑100 (MQ=255)
                                                                                  gtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTa             <  2:612070/100‑1 (MQ=255)
                                                                                  gtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTa             <  2:897481/100‑1 (MQ=255)
                                                                                     aTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaat          <  2:2239076/100‑1 (MQ=255)
                                                                                         cGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATg      >  1:2247274/1‑100 (MQ=255)
                                                                                            aTCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcgc   >  2:2325692/1‑100 (MQ=255)
                                                                                            aTCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcgc   >  2:2324460/1‑100 (MQ=255)
                                                                                             tCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGcgcg  <  2:1616318/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCG  >  NC_000913/2191335‑2191527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: