Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,264	G→A	W297* (TGG→TGA)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,264	0	G	A	100.0%	269.6 / NA	75	W297* (TGG→TGA)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/38);  total (37/38)

ATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGG  >  NC_000913/2485167‑2485360                                                                                                  |                                                                                                 aTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAt                                                                                                <  2:782170/100‑1 (MQ=255)  tGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAAtt                                                                                               >  1:996653/1‑100 (MQ=255)   gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTa                                                                                              <  1:1068968/100‑1 (MQ=255)   gAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTa                                                                                              <  2:1623542/100‑1 (MQ=255)      gTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagc                                                                                           >  1:553535/1‑100 (MQ=255)        tCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTAGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcag                                                                                         <  2:355003/100‑1 (MQ=255)         cGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAagcagc                                                                                        <  2:2322299/100‑1 (MQ=255)           cTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCAt                                                                                      >  1:1661293/1‑100 (MQ=255)            tATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATc                                                                                     <  1:2035143/100‑1 (MQ=255)             aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGACTTAAGCAGCATcc                                                                                    >  1:862459/1‑100 (MQ=255)             aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATcc                                                                                    <  1:1068357/100‑1 (MQ=255)             aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATcc                                                                                    <  1:881200/100‑1 (MQ=255)             aTGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATcc                                                                                    <  1:1966496/100‑1 (MQ=255)               gacgTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGCACCTGAAAAACAGTGAATTAAGCAGCATcc                                                                                    >  2:1069058/4‑98 (MQ=255)                aaGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCaa                                                                                 <  1:1903599/100‑1 (MQ=255)                      cacaAAATTGGCTTGATACAGGAAACCTGGCTTTTCTGAACAAACCATTAGTTTTCACTGAACTTGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                           <  1:1365951/100‑1 (MQ=255)                      cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                           <  2:2332205/100‑1 (MQ=255)                      cacaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTaaa                                                                           >  1:736903/1‑100 (MQ=255)                       acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                          >  2:1871495/1‑100 (MQ=255)                       acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                          >  2:2290093/1‑100 (MQ=255)                       acaAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAg                                                                          >  1:1399401/1‑100 (MQ=255)                        caAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAgg                                                                         >  1:1873429/1‑100 (MQ=255)                           aaTTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGc                                                                      <  1:965669/100‑1 (MQ=255)                            aTTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCt                                                                     >  1:691059/1‑100 (MQ=255)                             ttGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTg                                                                    <  2:1399398/100‑1 (MQ=255)                                gCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGaa                                                                 <  1:198670/100‑1 (MQ=255)                                 cTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGaaa                                                                >  1:2176440/1‑100 (MQ=255)                                  ttGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATGAAGCAGCATCCCAATTTAAAGGTGCTGGaaa                                                                <  1:1055146/99‑1 (MQ=255)                                   tGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAAt                                                              >  2:1147496/1‑100 (MQ=255)                                   tGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAAt                                                              >  2:1297084/1‑100 (MQ=255)                                    gATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATc                                                             <  2:546503/100‑1 (MQ=255)                                     aTACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATcc                                                            <  2:1976351/100‑1 (MQ=255)                                     aTACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATcc                                                            <  1:1067061/100‑1 (MQ=255)                                      tACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCt                                                           >  1:83570/1‑100 (MQ=255)                                        cAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTa                                                         >  2:2130001/1‑100 (MQ=255)                                          ggAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACt                                                       >  1:1809333/1‑100 (MQ=255)                                            aaaCCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCg                                                     >  2:1764908/1‑100 (MQ=255)                                               ccTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGccc                                                  <  1:1159205/100‑1 (MQ=255)                                                cTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGcccc                                                 <  1:1615103/100‑1 (MQ=255)                                                    ccTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTa                                             >  1:2180191/1‑100 (MQ=255)                                                     cTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTAt                                            <  1:836860/100‑1 (MQ=255)                                                      tttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTAtt                                           <  1:2290102/100‑1 (MQ=255)                                                       ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTc                                          <  2:1913106/100‑1 (MQ=255)                                                       ttCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTc                                          <  2:164296/100‑1 (MQ=255)                                                         cTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCTCCCTATTCTa                                        >  1:562734/1‑100 (MQ=255)                                                             aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                    <  2:831037/100‑1 (MQ=255)                                                             aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                    <  1:1147498/100‑1 (MQ=255)                                                             aCAAACCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGAc                                    <  2:2008620/100‑1 (MQ=255)                                                              cAAACCATTAGAACAAACTAAAAAAGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACg                                   <  2:1809328/100‑1 (MQ=255)                                                                aaCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCC‑TACTCGCCCCCCTATTCTATGACGGAt                                <  2:1832615/100‑1 (MQ=255)                                                                 aCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                                >  1:1074964/1‑100 (MQ=255)                                                                 aCCATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGAt                                <  1:2245814/100‑1 (MQ=255)                                                                  ccATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATg                               <  2:1661288/100‑1 (MQ=255)                                                                   cATTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGa                              <  1:2282994/100‑1 (MQ=255)                                                                    aTTAGAACTCACTGAACATGAAAAACAGTGAATTTAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGaa                             >  2:422210/1‑100 (MQ=255)                                                                    aTTAGAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGaa                             >  1:1419884/1‑100 (MQ=255)                                                                        gAACTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATg                         >  1:422017/1‑100 (MQ=255)                                                                          aCTCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGc                       <  2:2176433/100‑1 (MQ=255)                                                                            tCACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTc                     >  1:441668/1‑100 (MQ=255)                                                                             cACTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCg                    <  1:1069060/100‑1 (MQ=255)                                                                               cTGAACATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGt                  >  1:2271298/1‑100 (MQ=255)                                                                                 gAACATGAAAAACAGTGAATTAAGCAGCATCCCGATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTc                >  2:1545767/1‑100 (MQ=255)                                                                                  aaCATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCGCCTATTCTATGACGGATGAAAATggctcggctc                >  2:1565795/1‑99 (MQ=255)                                                                                   aCATGAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCgg              <  2:2877/100‑1 (MQ=255)                                                                                       gAAAAACAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCg          >  2:1601549/1‑100 (MQ=255)                                                                                         aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt        >  2:1184790/1‑100 (MQ=255)                                                                                         aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt        <  2:1873423/100‑1 (MQ=255)                                                                                         aaaaCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGtt        >  2:575755/1‑100 (MQ=255)                                                                                           aaCAGTGAATTAAACAGCATCCCAATTTAAACGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTAt      >  1:1578112/1‑100 (MQ=255)                                                                                            aCAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATg     >  1:658109/1‑100 (MQ=255)                                                                                             cAGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgg    <  2:1632111/100‑1 (MQ=255)                                                                                              aGTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATggg   >  2:1833034/1‑100 (MQ=255)                                                                                               gTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg  >  2:1984461/1‑100 (MQ=255)                                                                                               gTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg  >  1:1095862/1‑100 (MQ=255)                                                                                               gTGAATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATgggg  >  2:940157/1‑100 (MQ=255)                                                                                                  |                                                                                                 ATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGG  >  NC_000913/2485167‑2485360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑