Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,564,525 G→A M1M (ATG→ATA) † yffS → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,564,5250GA97.8% 315.3 / ‑2.4 92M1M (ATG→ATA) †yffSCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (49/41);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCA  >  NC_000913/2564428‑2564621
                                                                                                 |                                                                                                
tccgatctcGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAg                                                                                                <  1:702171/92‑1 (MQ=255)
gtgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCACTGGATTCTATGAGATGGGAGATAAAAATCATAAg                                                                                                >  2:1891392/1‑100 (MQ=255)
gtgATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAg                                                                                                <  1:1830709/100‑1 (MQ=255)
 tgATCTGCCTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGt                                                                                               >  1:458640/1‑100 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                                              >  1:458788/1‑100 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                                              >  2:156198/1‑100 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                                              >  2:1853250/1‑100 (MQ=255)
  atcttGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGtt                                                                                              <  2:1737644/96‑1 (MQ=255)
     cTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACg                                                                                           >  2:1973779/1‑100 (MQ=255)
     cTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACg                                                                                           <  2:1330344/100‑1 (MQ=255)
      tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTagat                                                                                          >  1:1737649/1‑97 (MQ=255)
      tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGa                                                                                          >  2:207255/1‑100 (MQ=255)
       atctCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaa                                                                                         <  2:1211728/97‑1 (MQ=255)
         ttctgttgccccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                                       <  2:458388/91‑1 (MQ=255)
         gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                                       >  1:1515679/1‑100 (MQ=255)
         gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                                       <  1:207257/100‑1 (MQ=255)
         gTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAACACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAAt                                                                                       >  2:1256830/1‑100 (MQ=255)
           cAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGaaagat                                                                                     >  1:1211731/1‑97 (MQ=255)
           cAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCa                                                                                     >  2:365411/1‑100 (MQ=255)
              tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCacaa                                                                                  >  1:2084701/1‑100 (MQ=255)
              tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaaaa                                                                                  >  2:622875/1‑100 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                            <  2:2175721/100‑1 (MQ=255)
                     ttCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGa                                                                           <  2:1369948/100‑1 (MQ=255)
                     ttCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGa                                                                           <  2:1957151/100‑1 (MQ=255)
                      tCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGAc                                                                          <  2:525632/100‑1 (MQ=255)
                        cGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACAt                                                                        >  1:573549/1‑100 (MQ=255)
                         gTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATc                                                                       >  1:696741/1‑100 (MQ=255)
                          ttACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATcc                                                                      <  2:658464/100‑1 (MQ=255)
                           tACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCt                                                                     >  2:1731792/1‑100 (MQ=255)
                           tACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCt                                                                     <  2:1515675/100‑1 (MQ=255)
                              gCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaa                                                                  >  1:98728/1‑100 (MQ=255)
                               cTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaaa                                                                 <  1:622876/100‑1 (MQ=255)
                                 tACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAgg                                                               >  1:1887750/1‑100 (MQ=255)
                                  aCCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgc                                                              >  2:1293833/1‑100 (MQ=255)
                                   cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                             >  2:1006584/1‑100 (MQ=255)
                                     cTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCt                                                           >  1:1288918/1‑100 (MQ=255)
                                      tCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTg                                                          >  1:33266/1‑100 (MQ=255)
                                       ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                         <  1:1696320/100‑1 (MQ=255)
                                         accaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGa                                                       >  1:657958/1‑100 (MQ=255)
                                          ccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt                                                      <  2:692695/100‑1 (MQ=255)
                                          ccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt                                                      >  2:1029525/1‑100 (MQ=255)
                                            aaTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                                                    <  2:1887743/100‑1 (MQ=255)
                                            aaTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                                                    >  1:164786/1‑100 (MQ=255)
                                            aaTACGCGTGTTAACCTCCCAATGGATTCTATGAGATAAAAAAAAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATgg                                                    <  2:903600/100‑1 (MQ=255)
                                             aTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGa                                                   <  2:458786/100‑1 (MQ=255)
                                             aTACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGa                                                   <  1:1006586/100‑1 (MQ=255)
                                               aCGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                                 <  1:1383090/100‑1 (MQ=255)
                                                cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                                 <  1:1029527/99‑1 (MQ=255)
                                                   gtgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACa                                             >  2:282189/1‑100 (MQ=255)
                                                    tgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACaa                                            >  2:569640/1‑100 (MQ=255)
                                                    tgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACaa                                            <  1:579211/100‑1 (MQ=255)
                                                     gtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAat                                           <  1:19521/100‑1 (MQ=255)
                                                       tAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAatat                                         <  2:114249/100‑1 (MQ=255)
                                                        aaCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                                        >  2:151649/1‑100 (MQ=255)
                                                        aaCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                                        <  1:1891399/100‑1 (MQ=255)
                                                         ctcTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaa                                       <  2:1381521/98‑1 (MQ=255)
                                                         aCCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaa                                       >  1:2324785/1‑100 (MQ=255)
                                                           cTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATaaag                                     >  1:1381524/1‑98 (MQ=255)
                                                            tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                                    <  1:365413/100‑1 (MQ=255)
                                                               cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                                 <  2:1093102/100‑1 (MQ=255)
                                                               cAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg                                  >  2:2053181/1‑99 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  2:558854/1‑100 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                <  1:2062938/100‑1 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  1:913856/1‑100 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  1:1319439/1‑100 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  2:2176180/1‑100 (MQ=255)
                                                                 aTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTc                               <  2:371275/100‑1 (MQ=255)
                                                                 aTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTc                               <  2:78238/100‑1 (MQ=255)
                                                                   ggATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCaa                             >  1:371172/1‑100 (MQ=255)
                                                                        cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        <  1:155302/100‑1 (MQ=255)
                                                                        cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        >  2:1206293/1‑100 (MQ=255)
                                                                        cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        >  2:954977/1‑100 (MQ=255)
                                                                        cTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        <  2:164784/100‑1 (MQ=255)
                                                                         tATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTggg                       >  1:368588/1‑100 (MQ=255)
                                                                         tATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTggg                       <  2:425326/100‑1 (MQ=255)
                                                                         tATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTggg                       <  2:1684956/100‑1 (MQ=255)
                                                                          aTGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg                      >  1:2175775/1‑100 (MQ=255)
                                                                           tGAGATGGGAGATAAAAATCATAAGTTCCGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGc                     <  2:2084694/100‑1 (MQ=255)
                                                                             agaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCa                   >  2:1036506/1‑100 (MQ=255)
                                                                                 gggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg               <  2:1158576/100‑1 (MQ=255)
                                                                                 gggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg               >  1:165297/1‑100 (MQ=255)
                                                                                  ggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTGAACGTGGGGCCAAATGc              >  2:532887/1‑100 (MQ=255)
                                                                                   gagaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCg             <  1:2053188/100‑1 (MQ=255)
                                                                                     gaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCga            >  1:1052178/1‑99 (MQ=255)
                                                                                      aTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagg          <  2:1288915/100‑1 (MQ=255)
                                                                                       tAAAAATCATAAGTTACGAAATCAAAATTTGCTACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGTCGCTCAACGTGGTGCCAAATGCgagga         >  2:118845/1‑100 (MQ=255)
                                                                                       tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga         >  2:1984406/1‑100 (MQ=255)
                                                                                       tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga         >  1:2236194/1‑100 (MQ=255)
                                                                                         aaaaTCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGgaga       >  1:1063980/1‑100 (MQ=255)
                                                                                         aaaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGgaga       <  1:151651/100‑1 (MQ=255)
                                                                                             tCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGc   >  2:2116070/1‑100 (MQ=255)
                                                                                              cATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCa  >  1:606731/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCA  >  NC_000913/2564428‑2564621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: