BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,931	T→C	intergenic (‑71/+128)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,931	0	T	C	98.4%	438.1 / ‑3.1	125	intergenic (‑71/+128)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (69/54); total (71/54)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 127 total aligned reads displayed.

TACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCG  >  NC_000913/2817834‑2818027

tACTCTATCCGGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAccc                                                                                                <  1:1561093/100‑1 (MQ=255)

tACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAccc                                                                                                <  2:1412753/100‑1 (MQ=255)

 aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCg                                                                                               <  2:734370/100‑1 (MQ=255)

 aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCg                                                                                               >  1:830164/1‑100 (MQ=255)

  ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCgg                                                                                              >  1:236603/1‑100 (MQ=255)

  ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                              >  1:727687/1‑100 (MQ=255)

  ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                              >  2:1684804/1‑100 (MQ=255)

    ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGgga                                                                                            >  2:599778/1‑100 (MQ=255)

    ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGgga                                                                                            <  1:656077/100‑1 (MQ=255)

     tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGggag                                                                                           >  1:129942/1‑100 (MQ=255)

      aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGggagg                                                                                          <  2:500081/100‑1 (MQ=255)

      aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGggagg                                                                                          >  1:239912/1‑100 (MQ=255)

         cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGAtt                                                                                       >  2:280522/1‑100 (MQ=255)

            cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACt                                                                                    >  2:776013/1‑100 (MQ=255)

               aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGc                                                                                 <  1:1785117/100‑1 (MQ=255)

               aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGc                                                                                 <  1:1906456/100‑1 (MQ=255)

                gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGcc                                                                                <  1:287519/100‑1 (MQ=255)

                    cGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGc                                                                            <  1:402030/100‑1 (MQ=255)

                      gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCgg                                                                          >  1:769326/1‑100 (MQ=255)

                      gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCgg                                                                          >  1:2169897/1‑100 (MQ=255)

                        tGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCt                                                                        <  1:1800680/100‑1 (MQ=255)

                        tGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCt                                                                        <  2:196399/100‑1 (MQ=255)

                          cATCGGGAAAACTTATTCTACTGCCGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                      >  1:782943/1‑100 (MQ=255)

                             cGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                   >  1:41572/1‑100 (MQ=255)

                              gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                  <  2:1471711/100‑1 (MQ=255)

                              gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                  >  1:314424/1‑100 (MQ=255)

                                gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                <  2:362704/100‑1 (MQ=255)

                                   aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                             >  1:1963214/1‑100 (MQ=255)

                                   aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                             >  1:1961632/1‑100 (MQ=255)

                                   aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                             <  2:495/100‑1 (MQ=255)

                                    aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                            <  2:1799488/100‑1 (MQ=255)

                                    aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                            <  2:1799547/100‑1 (MQ=255)

                                     cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                           >  2:2200719/1‑100 (MQ=255)

                                     cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                           >  2:753713/1‑100 (MQ=255)

                                     cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                           >  2:1796418/1‑100 (MQ=255)

                                       tATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                         <  2:1695138/100‑1 (MQ=255)

                                        aTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                        <  2:154443/100‑1 (MQ=255)

                                        aTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                        <  1:582855/100‑1 (MQ=255)

                                        aTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                        >  2:822050/1‑100 (MQ=255)

                                           cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                      >  2:271327/1‑99 (MQ=255)

                                              ctgctgATATTGATACCGCTACTAAAGCCATATCAATGAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                  >  2:1251559/1‑100 (MQ=255)

                                               tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                 <  2:2016315/100‑1 (MQ=255)

                                               tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                 <  2:769325/100‑1 (MQ=255)

                                                gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                <  1:1152025/100‑1 (MQ=255)

                                                atctatatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                <  2:1999373/96‑1 (MQ=255)

                                                 ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                               <  2:402376/100‑1 (MQ=255)

                                                      atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                          >  2:483686/1‑100 (MQ=255)

                                                       ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcc                                         <  2:1934463/100‑4 (MQ=14)

                                                       ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcc                                         <  1:776014/100‑4 (MQ=11)

                                                       ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                         <  2:1583992/100‑1 (MQ=255)

                                                       tggATACCGCTACAAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                         >  1:1583997/3‑100 (MQ=255)

                                                        tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                        >  1:2288674/1‑100 (MQ=255)

                                                        tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                        >  2:1038068/1‑100 (MQ=25)

                                                         gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                       <  1:822052/100‑1 (MQ=255)

                                                         gATACCGCCTCTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCTCTTCGGGCCGTTGCTTTAGCAActgccc                                        <  1:1017178/99‑6 (MQ=11)

                                                          aTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatca                                      <  1:1750029/100‑1 (MQ=255)

                                                           tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                     <  1:1251562/100‑1 (MQ=255)

                                                           tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                     >  2:1689128/1‑100 (MQ=255)

                                                             ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                   <  1:1010798/100‑1 (MQ=255)

                                                              cGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                  >  1:148137/1‑100 (MQ=255)

                                                              cGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                  >  1:149969/1‑100 (MQ=255)

                                                                  actaAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                              >  2:626469/1‑100 (MQ=255)

                                                                   ctaAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                             >  1:128601/1‑100 (MQ=255)

                                                                    taAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                            >  1:326914/1‑100 (MQ=255)

                                                                     aaaGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                           <  1:280524/100‑1 (MQ=255)

                                                                        gCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                        <  2:2315184/100‑1 (MQ=255)

                                                                        gCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                        >  2:1629092/1‑100 (MQ=255)

                                                                         cgatctCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                       <  2:1261436/95‑1 (MQ=255)

                                                                         ccATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                       <  1:421444/100‑1 (MQ=255)

                                                                          cATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                      <  2:1611993/100‑1 (MQ=255)

                                                                           atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                     <  2:864998/100‑1 (MQ=255)

                                                                           atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                     >  2:903017/1‑100 (MQ=255)

                                                                           atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                     <  1:271329/100‑1 (MQ=255)

                                                                           atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                     <  1:599779/100‑1 (MQ=255)

                                                                               cAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgagatcg                 >  1:1261439/1‑94 (MQ=255)

                                                                               cAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                 <  2:700840/100‑1 (MQ=255)

                                                                               cAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCAGCTCGCCCTTCGGGCCGTTACTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                 >  2:1276524/1‑100 (MQ=255)

                                                                                aaTTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAtt                >  1:1556835/1‑100 (MQ=255)

                                                                                 aTTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc               >  1:1032019/1‑100 (MQ=255)

                                                                                   tAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg             >  2:631267/1‑100 (MQ=255)

                                                                                   tAAGAGAAGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg             >  1:1234855/1‑100 (MQ=255)

                                                                                    cttccgatctGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc            <  2:2271385/91‑1 (MQ=17)

                                                                                    aaGAGATGGTGCACCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc            <  1:1038070/100‑1 (MQ=255)

                                                                                    aaGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc            >  2:2207271/1‑100 (MQ=255)

                                                                                     agagATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt           >  1:2207025/1‑100 (MQ=255)

                                                                                     agagATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt           <  1:461156/100‑1 (MQ=255)

                                                                                     agagATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt           >  1:762035/1‑100 (MQ=255)

                                                                                     agagATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt           >  1:1901238/1‑100 (MQ=255)

                                                                                      gagaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt           >  2:403215/1‑99 (MQ=255)

                                                                                      gagaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa          >  1:586939/1‑100 (MQ=255)

                                                                                      gagaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa          <  2:239910/100‑1 (MQ=255)

                                                                                        gaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc        <  1:2200727/100‑1 (MQ=255)

                                                                                         aTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct       >  1:965095/1‑100 (MQ=255)

                                                                                          tGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctc      >  2:1036986/1‑100 (MQ=255)

                                                                                            gTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt    <  2:41570/100‑1 (MQ=255)

                                                                                            gTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt    <  2:1183271/100‑1 (MQ=255)

                                                                                            gTGCACCCGGGAGGATTACTAGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctcat    >  2:1423618/1‑98 (MQ=255)

                                                                                             tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc   <  2:1893451/100‑1 (MQ=255)

                                                                                              gCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg  >  1:1635427/1‑100 (MQ=255)

                                                                                              gCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg  <  2:149967/100‑1 (MQ=255)

TACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCG  >  NC_000913/2817834‑2818027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`