Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,582,272 G→A C137Y (TGT→TAT)  yhhZ → putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,582,2720GA98.9% 324.4 / NA 91C137Y (TGT→TAT) yhhZputative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (41/49);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATA  >  NC_000913/3582176‑3582367
                                                                                                |                                                                                               
aaCTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCa                                                                                              <  1:1383435/100‑1 (MQ=255)
   tAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACa                                                                                           <  2:270179/100‑1 (MQ=255)
    aaGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATAAAGAAACAATAACTATTAGTTATGAATTTATACTCTATCAACAt                                                                                          >  1:1158087/1‑100 (MQ=255)
     agagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATc                                                                                         >  1:1070475/1‑100 (MQ=255)
      gagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCt                                                                                        <  2:2269619/100‑1 (MQ=255)
      gagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCt                                                                                        <  2:869467/100‑1 (MQ=255)
      gagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCt                                                                                        <  2:1127704/100‑1 (MQ=255)
        ggCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTa                                                                                      >  1:499135/1‑100 (MQ=255)
        ggCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTa                                                                                      >  2:908239/1‑100 (MQ=255)
          cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATc                                                                                    >  2:1799595/1‑100 (MQ=255)
          cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATc                                                                                    >  2:895043/1‑100 (MQ=255)
          cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATc                                                                                    >  2:1622340/1‑100 (MQ=255)
          cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATc                                                                                    >  2:1990944/1‑100 (MQ=255)
           gcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCg                                                                                   <  2:128930/100‑1 (MQ=255)
            cTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCg                                                                                   <  2:2276007/99‑1 (MQ=255)
             ttttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCa                                                                                 <  1:2254287/100‑1 (MQ=255)
             ttttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCa                                                                                 <  1:726271/100‑1 (MQ=255)
             ttttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCa                                                                                 >  1:1879533/1‑100 (MQ=255)
               ttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCaaa                                                                               <  1:1077871/100‑1 (MQ=255)
                 ttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATa                                                                             <  1:1615288/100‑1 (MQ=255)
                  tATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATAc                                                                            >  2:1873386/1‑100 (MQ=255)
                    tCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCg                                                                          <  2:1703998/100‑1 (MQ=255)
                    tCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCg                                                                          <  1:169053/100‑1 (MQ=255)
                     cGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGa                                                                         >  1:2105606/1‑100 (MQ=255)
                     cGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGa                                                                         >  2:1212026/1‑100 (MQ=255)
                      ggCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAg                                                                        >  1:2156449/1‑100 (MQ=255)
                      ggCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAg                                                                        >  1:1248876/1‑100 (MQ=255)
                         tATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTc                                                                     <  1:1634816/100‑1 (MQ=255)
                         tATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTc                                                                     <  1:1128133/100‑1 (MQ=255)
                          aTTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCa                                                                    <  2:2311721/100‑1 (MQ=255)
                          aTTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCa                                                                    >  1:1165826/1‑100 (MQ=255)
                              atcaCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTa                                                                <  1:1075144/100‑1 (MQ=255)
                               tcaCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTAt                                                               >  2:800/1‑100 (MQ=255)
                                caCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTAtt                                                              >  2:2229427/1‑100 (MQ=255)
                                 aCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTAttt                                                             <  1:800/100‑1 (MQ=255)
                                 aCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTAttt                                                             >  2:1056349/1‑100 (MQ=255)
                                   cAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTgg                                                           >  2:318743/1‑100 (MQ=255)
                                    aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc                                                          <  2:232488/100‑1 (MQ=255)
                                    aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc                                                          <  1:1744761/100‑1 (MQ=255)
                                    aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc                                                          >  2:985244/1‑100 (MQ=255)
                                           tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCt                                                   >  2:2018427/1‑100 (MQ=255)
                                           tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCt                                                   >  2:685120/1‑100 (MQ=255)
                                           tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCt                                                   <  1:1641870/100‑1 (MQ=255)
                                           tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCt                                                   <  1:1194296/100‑1 (MQ=255)
                                            gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTg                                                  >  2:1291001/1‑100 (MQ=255)
                                            gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTg                                                  <  1:914683/100‑1 (MQ=255)
                                            gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTg                                                  >  2:1034397/1‑100 (MQ=255)
                                               aaCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGaaa                                               >  1:2274390/1‑100 (MQ=255)
                                               aaCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGaaa                                               <  2:926413/100‑1 (MQ=255)
                                                     cTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATtata                                         <  1:1034399/100‑1 (MQ=255)
                                                      tGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATaa                                        >  2:309805/1‑100 (MQ=255)
                                                       ggATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAc                                       <  1:895045/100‑1 (MQ=255)
                                                       ggATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAc                                       >  2:719001/1‑100 (MQ=255)
                                                        gATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAcc                                      <  1:1872218/100‑1 (MQ=255)
                                                        gATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAcc                                      <  2:1820845/100‑1 (MQ=255)
                                                            cAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgttt                                  <  1:2004275/100‑1 (MQ=255)
                                                             aGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttg                                 <  1:2206782/100‑1 (MQ=255)
                                                             aGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttg                                 >  1:229834/1‑100 (MQ=255)
                                                                 aCAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGtttt                             >  1:2302930/1‑100 (MQ=255)
                                                                  cAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGttttt                            <  2:732905/100‑1 (MQ=255)
                                                                  cAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGttttt                            <  2:1070473/100‑1 (MQ=255)
                                                                  cAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATCATAACCGTTTGttttt                            <  2:589559/100‑1 (MQ=255)
                                                                    aTAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAc                          <  2:2274382/100‑1 (MQ=255)
                                                                     tAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAcc                         <  1:1622345/100‑1 (MQ=255)
                                                                     tAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAcc                         <  1:318745/100‑1 (MQ=255)
                                                                     tAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGCTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAcc                         <  1:1450509/100‑1 (MQ=255)
                                                                       aCTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCaa                       <  1:1342015/100‑1 (MQ=255)
                                                                         tATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAAt                     <  2:1970518/100‑1 (MQ=255)
                                                                           ttAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTc                   >  2:327973/1‑100 (MQ=255)
                                                                           ttAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTc                   <  2:1446790/100‑1 (MQ=255)
                                                                            tAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCa                  <  2:2156442/100‑1 (MQ=255)
                                                                             aGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTGCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa                 <  2:1248873/100‑1 (MQ=255)
                                                                              gTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaa                >  2:597064/1‑100 (MQ=255)
                                                                                tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa              <  1:2229435/100‑1 (MQ=255)
                                                                                tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa              <  1:2092432/100‑1 (MQ=255)
                                                                                tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACAGTTTGTTTTTACCAAATTCaaaaa              >  2:333147/1‑100 (MQ=255)
                                                                                 ntGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa             >  1:435229/2‑100 (MQ=255)
                                                                                 aTGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa             <  1:719002/100‑1 (MQ=255)
                                                                                 aTGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa             >  1:234975/1‑100 (MQ=255)
                                                                                 aTGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa             <  1:658632/100‑1 (MQ=255)
                                                                                  tGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAc            >  1:188668/1‑100 (MQ=255)
                                                                                  tGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAc            >  1:1750333/1‑100 (MQ=255)
                                                                                  tGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAc            <  1:1990951/100‑1 (MQ=255)
                                                                                    aaTTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACca          >  1:1647639/1‑100 (MQ=255)
                                                                                    aaTTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACca          >  2:888103/1‑100 (MQ=255)
                                                                                     aTTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaa         >  2:823367/1‑100 (MQ=255)
                                                                                       ttATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaac       <  1:327975/100‑1 (MQ=255)
                                                                                        tATCCTCTATCAACATCTTATCCCAAATACCGAGTTCAGATATTTGGCCCTCCCTAAAAATTATAACCGTTTGTTTTTACCAAATTAAAAAAACcaaaca      <  2:556774/100‑1 (MQ=255)
                                                                                           ccTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaat   >  1:1486261/1‑100 (MQ=255)
                                                                                           ccTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaat   >  1:1002574/1‑100 (MQ=255)
                                                                                            ctctATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaata  >  1:886177/1‑100 (MQ=255)
                                                                                                |                                                                                               
AACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATA  >  NC_000913/3582176‑3582367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑