Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,594,073 | G→T | T45N (ACC→AAC) | livG ← | branched‑chain amino acid ABC transporter ATPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,594,073 | 0 | G | T | 94.7% | 192.1 / 3.2 | 58 | T45N (ACC→AAC) | livG | branched‑chain amino acid ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base G (3/0); new base T (32/22); total (36/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 |
AATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCG > NC_000913/3593976‑3594167 | aaTTTGCTGCCACGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGttt < 2:925655/100‑1 (MQ=255) tttGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTtttt > 1:67407/1‑100 (MQ=255) tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCgg < 1:1954938/100‑1 (MQ=255) gcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGc < 2:2178503/100‑1 (MQ=255) ggTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGt > 1:2311259/1‑100 (MQ=255) ggTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGt > 1:483083/1‑100 (MQ=255) aCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAggg > 2:1837246/1‑100 (MQ=255) ccAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGAt > 1:2249257/1‑100 (MQ=255) cAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGAtt < 1:1974058/100‑1 (MQ=255) gTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAAc < 1:982927/100‑1 (MQ=255) tGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACg < 1:378702/100‑1 (MQ=255) tGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACg < 1:301234/100‑1 (MQ=255) gCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACga > 1:2252706/1‑100 (MQ=255) tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACgaga > 2:2266737/1‑100 (MQ=255) gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc > 2:2191637/1‑100 (MQ=255) gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc > 2:1941469/1‑100 (MQ=255) gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc > 2:169566/1‑100 (MQ=255) gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGAc > 1:2154947/1‑100 (MQ=255) gcgcAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtc > 1:2114958/1‑100 (MQ=255) aGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCt < 1:702665/100‑1 (MQ=255) gTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTg > 1:440473/1‑100 (MQ=255) aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCgg < 2:597384/100‑1 (MQ=255) aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCgg > 1:908516/1‑100 (MQ=255) aTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGt < 1:464747/100‑1 (MQ=255) tGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAg > 2:630942/1‑100 (MQ=255) gccgccGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTCCGGGTACGGt > 1:2040146/1‑100 (MQ=255) cgccgGTGGGTTTGTAGATTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGGACAGTTc > 2:2183568/1‑100 (MQ=255) cgccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTc > 1:1182284/1‑100 (MQ=255) ggTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGa > 2:287157/1‑100 (MQ=255) gTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGAt < 1:2266745/100‑1 (MQ=255) gggTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTg > 2:1465425/1‑100 (MQ=255) ggTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGa < 2:2154940/100‑1 (MQ=255) gTTTGTAGAATCCGGTCAGACAGTTAAAATCCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGa < 1:115527/99‑1 (MQ=255) gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc > 2:1900753/1‑100 (MQ=255) tttGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACg < 1:1158567/100‑1 (MQ=255) tGTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt > 1:503940/1‑100 (MQ=255) gTAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttg < 1:1941476/100‑1 (MQ=255) tAGAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt < 2:51780/100‑1 (MQ=255) gAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTc < 1:1465429/100‑1 (MQ=255) gAATCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTc > 1:1322883/1‑100 (MQ=255) aTCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc > 2:1178388/1‑100 (MQ=255) aTCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc > 1:690982/1‑100 (MQ=255) aTCCGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGACCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc > 1:1342187/1‑100 (MQ=255) cGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGc > 1:992466/1‑100 (MQ=255) cGGTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGc > 2:1331232/1‑100 (MQ=255) ggTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGcc > 1:1923832/1‑100 (MQ=255) gTCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGcc < 2:67405/99‑1 (MQ=255) tCAGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcag < 2:503938/100‑1 (MQ=255) aGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca < 2:2311250/100‑1 (MQ=255) aGACAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca < 1:169568/100‑1 (MQ=255) aCAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAgg > 1:1472434/1‑100 (MQ=255) cAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGCCGCTCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca > 1:1151124/1‑97 (MQ=255) cAGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgc > 2:67686/1‑100 (MQ=255) aGTTAAAAACCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgcc > 2:391319/1‑100 (MQ=255) gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg > 2:830344/1‑100 (MQ=255) gTTAAAAACCGTGATTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGgccg > 1:1571192/1‑100 (MQ=255) aaaaaCCGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGccgccg < 1:242103/100‑1 (MQ=255) aaaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGccgccg > 1:1409528/1‑100 (MQ=255) ccGTGTTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgcg < 1:1367986/100‑1 (MQ=255) | AATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCG > NC_000913/3593976‑3594167 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |