Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,853,909	A→G	intergenic (+267/‑13)	tisB → / → emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,853,909	0	A	G	99.0%	354.4 / ‑4.4	98	intergenic (+267/‑13)	tisB/emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (46/51);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853812‑3854004                                                                                                  |                                                                                                ttCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGc                                                                                               >  2:1630040/1‑100 (MQ=255)  tCCTGGCGTATATCTGTCTAACATTCGTCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCa                                                                                              <  2:1881721/100‑1 (MQ=255)    cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATc                                                                                            >  1:2115645/1‑100 (MQ=255)    cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATc                                                                                            <  2:50125/100‑1 (MQ=255)     tGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATcc                                                                                           >  1:1837758/1‑100 (MQ=255)       gCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGt                                                                                         <  1:2207077/100‑1 (MQ=255)        cGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTg                                                                                        <  1:1653000/100‑1 (MQ=255)        cGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTg                                                                                        >  1:1780140/1‑100 (MQ=255)             atCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAt                                                                                   <  2:1837753/100‑1 (MQ=255)              tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  <  2:2312092/100‑1 (MQ=255)                tGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGGAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  <  2:1896155/98‑1 (MQ=255)                 ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaa                                                                               >  1:2231878/1‑100 (MQ=255)                 ggCTAACATTCATCAACGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaa                                                                                >  2:476372/1‑99 (MQ=255)                   cTAACATTCATCAGTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAg                                                                             <  2:1527323/100‑1 (MQ=255)                       cATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCaa                                                                         >  2:1312769/1‑100 (MQ=255)                         ttCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAg                                                                       <  2:1316275/100‑1 (MQ=255)                          tcatcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGa                                                                      >  2:1759343/1‑100 (MQ=255)                          tcatcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGa                                                                      >  2:1185150/1‑100 (MQ=255)                           catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa                                                                     >  2:47251/1‑100 (MQ=255)                           catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa                                                                     >  1:1445839/1‑100 (MQ=255)                             tcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAAc                                                                   <  2:1921887/100‑1 (MQ=255)                             tcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAAc                                                                   >  1:2177839/1‑100 (MQ=255)                              caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg                                                                  >  2:394203/1‑100 (MQ=255)                               aaTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGt                                                                 >  1:529051/1‑100 (MQ=255)                                 tgtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCa                                                               >  1:977655/1‑100 (MQ=255)                                  gtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa                                                              >  2:160717/1‑100 (MQ=255)                                      tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg                                                          >  2:2060458/1‑100 (MQ=255)                                       aGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAtttat                                                         <  1:1984240/100‑3 (MQ=255)                                       aGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt                                                         <  2:731614/100‑1 (MQ=255)                                       aGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt                                                         <  2:1292452/100‑1 (MQ=255)                                        gATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAACGGCATCCGTGATAATGAAAAGGCAAAGAAACGGCAATTTGt                                                         >  1:1414996/1‑99 (MQ=255)                                        gATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGtt                                                        >  2:1008976/1‑100 (MQ=255)                                           tCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATTCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAtt                                                     <  2:1058354/100‑1 (MQ=255)                                            ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    <  1:316341/100‑1 (MQ=255)                                            ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    <  1:345853/100‑1 (MQ=255)                                            ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    >  2:1469788/1‑100 (MQ=255)                                            ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    >  2:855973/1‑100 (MQ=255)                                            ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    >  1:106551/1‑100 (MQ=255)                                             ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   >  2:2060954/1‑100 (MQ=255)                                             ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   <  2:2176055/100‑1 (MQ=255)                                             ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   <  2:977653/100‑1 (MQ=255)                                                tcCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                                                <  2:2278346/100‑1 (MQ=255)                                                tcCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                                                <  2:1751058/100‑1 (MQ=255)                                                tcCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                                                <  1:47253/100‑1 (MQ=255)                                                 cccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGtt                                               <  1:1161536/100‑1 (MQ=255)                                                  ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg                                              >  1:1840501/1‑100 (MQ=255)                                                  ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg                                              <  1:779725/100‑1 (MQ=255)                                                   cGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTgg                                             <  2:1507271/100‑1 (MQ=255)                                                    gCATTTATGGGAATGCGTAGTGACTTATTCTAACTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGt                                            <  2:373476/100‑1 (MQ=255)                                                     cATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTa                                           <  1:2060961/100‑1 (MQ=255)                                                      aTTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTAGAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTgg                                             <  2:1490641/97‑1 (MQ=255)                                                       tttATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAtt                                         >  1:1930595/1‑100 (MQ=255)                                                       tttATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAtt                                         <  2:2231870/100‑1 (MQ=255)                                                            gggAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCg                                    >  2:1080064/1‑100 (MQ=255)                                                             ggAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGt                                   >  2:317783/1‑100 (MQ=255)                                                              gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  2:2177832/100‑1 (MQ=255)                                                               aaTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTgg                                 <  1:855975/100‑1 (MQ=255)                                                                cgatctAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  2:615597/95‑1 (MQ=255)                                                                aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  1:1088079/100‑1 (MQ=255)                                                                aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  2:323056/100‑1 (MQ=255)                                                                  gCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCg                              >  2:1118861/1‑100 (MQ=255)                                                                   cGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGt                             >  2:181672/1‑100 (MQ=255)                                                                   cGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGt                             >  2:14542/1‑100 (MQ=255)                                                                    gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  1:390704/100‑1 (MQ=255)                                                                    gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  2:1840496/100‑1 (MQ=255)                                                                    gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  1:1505823/100‑1 (MQ=255)                                                                      aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg                          <  1:1223085/100‑1 (MQ=255)                                                                      aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg                          <  1:881963/100‑1 (MQ=255)                                                                      aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAGTGATGTTGGTATTACTCGTGGcagatcg                          >  1:615598/1‑94 (MQ=255)                                                                         gACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa                       >  2:1055914/1‑100 (MQ=255)                                                                         gACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa                       >  1:1968433/1‑100 (MQ=255)                                                                          aCTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAg                      <  2:1125797/100‑1 (MQ=255)                                                                          aCTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAg                      <  2:1125879/100‑1 (MQ=255)                                                                          aCTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAg                      <  1:1630045/100‑1 (MQ=255)                                                                           cTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGa                     >  2:2012832/1‑100 (MQ=255)                                                                              aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATgg                  <  1:853074/100‑1 (MQ=255)                                                                              aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATgg                  >  2:332249/1‑100 (MQ=255)                                                                               ttCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgc                 >  1:2038429/1‑100 (MQ=255)                                                                               ttCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgc                 >  1:2038440/1‑100 (MQ=255)                                                                                tCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg                >  2:2142729/1‑100 (MQ=255)                                                                                cttAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg                <  2:91894/98‑1 (MQ=255)                                                                                 cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               >  2:1960710/1‑100 (MQ=255)                                                                                 cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               <  2:2142750/100‑1 (MQ=255)                                                                                  tAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgag              >  1:91896/1‑98 (MQ=255)                                                                                  tAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCa              <  1:2037219/100‑1 (MQ=255)                                                                                   acttattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaa             <  2:1414992/98‑1 (MQ=255)                                                                                   aaTTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaa             >  2:664523/1‑100 (MQ=255)                                                                                    attattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaaa            >  1:1510902/1‑100 (MQ=255)                                                                                    attattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaaa            >  2:797497/1‑100 (MQ=255)                                                                                      tattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAcc          >  2:1818688/1‑100 (MQ=255)                                                                                      tattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAcc          >  1:1517539/1‑100 (MQ=255)                                                                                          tttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAttt      <  1:1800199/100‑1 (MQ=255)                                                                                          tttATAAAGGCATCCGTGATAATGAAAAGACAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAttt      <  2:718730/100‑1 (MQ=255)                                                                                          tttATAAAGGCAGCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAt        <  2:2333351/98‑1 (MQ=255)                                                                                           ttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     <  1:1759348/100‑1 (MQ=255)                                                                                             ataAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTtata   <  2:2115638/100‑1 (MQ=255)                                                                                              taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  <  1:160719/100‑1 (MQ=255)                                                                                              taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  >  1:951963/1‑100 (MQ=255)                                                                                                  |                                                                                                TTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853812‑3854004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑