Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,731 Δ1 bp 100% coding (199/1023 nt) ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,7280A.52.1% 160.0 / 217.9 94coding (202/1023 nt)ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (24/21);  new base . (21/28);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 104 total aligned reads displayed.

GCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATC  >  NC_000913/1560629‑1560827
                                                                                                   |                                                                                                   
gCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTa                                                                                                     <  2:1585123/100‑1 (MQ=255)
 cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaa                                                                                                    <  2:2786728/100‑1 (MQ=255)
  gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaa                                                                                                   <  1:2808600/100‑1 (MQ=255)
   tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaac                                                                                                  <  2:1497089/100‑2 (MQ=255)
   tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaac                                                                                                  <  2:2447108/100‑2 (MQ=255)
     gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaactg                                                                                                >  2:3475504/1‑97 (MQ=255)
     gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACt                                                                                                >  1:3578120/1‑100 (MQ=255)
     gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGCAGCGGTaaactg                                                                                                <  1:2712402/100‑4 (MQ=255)
     gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGATACGAATGGATGTTCCCAGGTCACCATGAAACAAGTCGCTGACGTAGCGGTAAAAc                                                                                                 >  2:614725/1‑99 (MQ=255)
      ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaactgt                                                                                               <  2:1265888/100‑5 (MQ=255)
       cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGTATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTaaactgt                                                                                               <  2:2397034/99‑5 (MQ=255)
       cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCCCCATGAAACAGGTCGCTGACGTAGCGGTAAACTGta                                                                                             <  2:2268298/100‑1 (MQ=255)
        gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGta                                                                                             >  1:29766/1‑100 (MQ=255)
        gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGta                                                                                             <  2:4017954/100‑1 (MQ=255)
        gggAAAAATATACGCAACTCTTCCAGCACCCGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtac                                                                                            <  1:2781622/100‑1 (MQ=255)
          gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtacat                                                                                          >  1:129794/1‑100 (MQ=255)
            aaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtacatac                                                                                        <  2:2911923/100‑1 (MQ=255)
             aaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGtacataca                                                                                       >  1:3658288/1‑100 (MQ=255)
             aaaTATACGCAACACGTCCAGCACCGGACGCCCGTTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGtacata                                                                                         <  1:2406340/99‑1 (MQ=255)
                tataCGCACCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGt                                                                                     <  2:3626428/100‑1 (MQ=255)
                tataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGt                                                                                     <  2:3078407/100‑1 (MQ=255)
                   aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCt                                                                                 <  1:4053988/100‑1 (MQ=255)
                    cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCt                                                                                 >  1:1586995/1‑100 (MQ=255)
                    cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCt                                                                                 >  1:1441212/1‑100 (MQ=255)
                    cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTg                                                                                >  1:895864/1‑100 (MQ=255)
                    cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTg                                                                                >  1:510453/1‑100 (MQ=255)
                      cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTgg                                                                               <  1:1310010/100‑1 (MQ=255)
                      cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGt                                                                              <  2:1324613/100‑1 (MQ=255)
                      cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGt                                                                              <  2:1320765/100‑1 (MQ=255)
                       aaCTCTTCCAGCACCGGTCGCGCGGTACGAATGGATGTTTCCAGGTCCCCATGAAACAGGTCGCTGACTTAGCGGTAAAACTGTACATACAGTGGCTGGt                                                                              <  1:1050812/100‑1 (MQ=255)
                        cgatctCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTcc                                                                            <  2:1266741/95‑1 (MQ=255)
                        aCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTcc                                                                            <  2:1732212/100‑1 (MQ=255)
                         ctctTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTcc                                                                            <  1:2908597/100‑1 (MQ=255)
                         ctctTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTcc                                                                            <  1:3035210/100‑1 (MQ=255)
                            ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAg                                                                          >  2:1155909/1‑99 (MQ=255)
                             tCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTc                                                                        >  1:759783/1‑100 (MQ=255)
                              ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAgatcg                                                                      >  1:1266749/1‑96 (MQ=255)
                                 gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGt                                                                   <  2:604727/100‑1 (MQ=255)
                                  cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGt                                                                   >  2:426234/1‑100 (MQ=255)
                                   aCCGGCCGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                  <  2:4058377/100‑1 (MQ=255)
                                   aCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCACGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTg                                                                 >  1:3219472/1‑100 (MQ=255)
                                      ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                              <  1:632910/100‑1 (MQ=255)
                                       gACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTgg                                                             <  1:3742802/100‑1 (MQ=255)
                                        aCGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGc                                                            <  1:568802/100‑1 (MQ=255)
                                          gCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACCGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                           <  2:2202396/100‑1 (MQ=255)
                                           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGaa                                                         >  1:3557955/1‑100 (MQ=255)
                                           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGaa                                                         <  1:1966994/100‑1 (MQ=255)
                                           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGaa                                                         <  1:1967872/100‑1 (MQ=255)
                                              ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAatat                                                      <  1:2565138/100‑1 (MQ=255)
                                               gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATAtt                                                     <  2:19906/100‑1 (MQ=255)
                                                    aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                                 >  2:4096574/1‑100 (MQ=255)
                                                    aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                                 <  2:3334629/100‑1 (MQ=255)
                                                    aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                >  1:2330570/1‑100 (MQ=255)
                                                     aTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAACACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                >  2:2343002/1‑100 (MQ=255)
                                                      tGGATGTTCCCAGGTCACCATGAAACAGGGCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCAc                                               <  2:1601315/100‑1 (MQ=255)
                                                          tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAg                                          <  1:1490402/100‑1 (MQ=255)
                                                          tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTACACGATAg                                          >  1:1629725/1‑100 (MQ=255)
                                                           gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAg                                          >  1:1427522/1‑100 (MQ=255)
                                                           gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAg                                          <  1:2670265/100‑1 (MQ=255)
                                                            ttCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                         <  1:687828/100‑1 (MQ=255)
                                                            ttCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                         >  2:3167498/1‑100 (MQ=255)
                                                              cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                       >  1:2694979/1‑100 (MQ=255)
                                                              cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                       >  2:1331612/1‑100 (MQ=255)
                                                              cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                       <  1:1146885/100‑1 (MQ=255)
                                                                cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCg                                     >  1:3705529/1‑100 (MQ=255)
                                                                cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGc                                    >  1:2230991/1‑100 (MQ=255)
                                                                  ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCt                                   <  1:256990/100‑1 (MQ=255)
                                                                  ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTg                                  >  1:2325674/1‑100 (MQ=255)
                                                                   gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTgg                                 <  1:288166/100‑1 (MQ=255)
                                                                   gTCACCATGAAACAGGTCGCGGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTg                                  <  2:1113317/99‑1 (MQ=255)
                                                                     cACCATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcg                               >  1:2819362/1‑100 (MQ=255)
                                                                       ccATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcg                               >  2:1231887/1‑98 (MQ=255)
                                                                       ccATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                             <  1:1997873/100‑1 (MQ=255)
                                                                       ccATGAAACAGGACGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcgc                              >  1:2717337/1‑100 (MQ=255)
                                                                        cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                             >  2:3926853/1‑99 (MQ=255)
                                                                        cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                            >  2:1026079/1‑100 (MQ=255)
                                                                        cATGAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                            <  1:1486814/100‑1 (MQ=255)
                                                                         aTGAAACGGGTCGATGGAGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCGCGGTTGCTGGCGAAGATCTTCCACGATAGCATCGCTGGCGCgg                            <  2:1997497/99‑1 (MQ=255)
                                                                           gAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTc                          >  1:3053351/1‑100 (MQ=255)
                                                                           gAAACAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGCTAGCATCGCTGGCGCGGTCa                         >  2:1988016/1‑100 (MQ=255)
                                                                           gAAACAGGTCGCTGACGTAGCGGTAAACTGGACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                            <  2:747559/97‑1 (MQ=255)
                                                                               cAGGTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCg                     <  1:2464372/100‑1 (MQ=255)
                                                                                aGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCg                     <  2:1441202/100‑1 (MQ=255)
                                                                                 ggTCGCTGTCGTAGCGGTAAACTGTACATACAGAGGCTGGTCCAGTGCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                   >  2:1438169/1‑100 (MQ=255)
                                                                                 ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                   >  2:1394468/1‑100 (MQ=255)
                                                                                 ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                   <  1:1230849/100‑1 (MQ=255)
                                                                                 ggTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                   <  1:4122622/100‑1 (MQ=255)
                                                                                  gTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                   >  1:4106709/1‑100 (MQ=255)
                                                                                  gTCGCTGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                  >  1:394869/1‑100 (MQ=255)
                                                                                     gCTGACGTAGCGGTAAACTATACATACACTGGCTAGTCCAGTCCCAGTCGCTGGCGAATATTTTCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTa               <  2:1677716/100‑1 (MQ=255)
                                                                                       tGACGTAGCGGTAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc             <  2:255214/100‑1 (MQ=255)
                                                                                        gACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc             >  1:2033242/1‑100 (MQ=255)
                                                                                        gACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc             >  1:2033243/1‑100 (MQ=255)
                                                                                              gCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGcc       >  2:3352327/1‑100 (MQ=255)
                                                                                               cGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCg      >  2:3441333/1‑100 (MQ=255)
                                                                                                ggTAAAGCTGTACATACAGTGGCTGGTCCAGTGCCAGGTGCTGGCTAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGcca      <  2:2602261/99‑2 (MQ=25)
                                                                                                ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccag     <  1:3267887/100‑3 (MQ=37)
                                                                                                ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCgg     <  1:2029322/100‑1 (MQ=255)
                                                                                                ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCgg     >  1:1042513/1‑100 (MQ=255)
                                                                                                 gTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa    <  1:1915831/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATC  >  NC_000913/1560629‑1560827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: