Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,555 Δ1 bp 16.0% coding (1126/1452 nt) uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,5550G.16.0% 292.4 / 54.9 100coding (1126/1452 nt)uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (44/40);  new base . (6/10);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 120 total aligned reads displayed.

ATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAC  >  NC_000913/1609456‑1609654
                                                                                                   |                                                                                                    
 tGTTTCGCCGTTACGCTCACCACGATAGCACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     <  2:3385978/100‑1 (MQ=255)
 tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     <  2:1464293/100‑1 (MQ=255)
  gattCGCCGTTACGCTCACCACGATAGAACGAAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGAAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     >  2:1422104/3‑99 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagca                                                                                                    <  2:1054233/100‑1 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagca                                                                                                    >  1:4092074/1‑100 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagca                                                                                                    >  2:1619666/1‑100 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagca                                                                                                    >  1:2978850/1‑100 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagca                                                                                                    >  1:1699246/1‑100 (MQ=255)
  gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     >  1:577947/1‑99 (MQ=255)
   tttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagcag                                                                                                   >  1:4030636/1‑100 (MQ=255)
    ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  >  1:95781/1‑100 (MQ=255)
    ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  <  1:2058349/100‑1 (MQ=255)
    ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  >  2:1865311/1‑100 (MQ=255)
    ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  <  1:1291090/100‑1 (MQ=255)
    ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  >  1:3267267/1‑100 (MQ=255)
     tCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCt                                                                                                 >  1:141591/1‑100 (MQ=255)
      cGCCGTTACGCGCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGACGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCt                                                                                                 >  2:2167425/1‑99 (MQ=255)
        ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                             >  2:3963305/1‑100 (MQ=255)
        ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                             <  2:2579412/100‑1 (MQ=255)
            tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAg                                                                                         <  2:742734/100‑1 (MQ=255)
            tACGCTCACCACGATAGAACGCAATCAATGCCGCTAAGGCGAAAGTACGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                         <  2:2748077/100‑1 (MQ=255)
             aCGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgg                                                                                        <  2:1551283/100‑1 (MQ=255)
              cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGa                                                                                       <  1:2653402/100‑1 (MQ=255)
              cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                       <  2:1301002/100‑2 (MQ=255)
              cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                       <  1:2277647/100‑2 (MQ=255)
              cGCTCACCACGAGAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgg                                                                                        <  2:393550/99‑1 (MQ=255)
               gCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggc                                                                                      <  1:2318444/100‑3 (MQ=255)
                cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATg                                                                                     >  1:1692133/1‑100 (MQ=255)
                cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATg                                                                                     >  1:2351499/1‑100 (MQ=255)
                cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                     <  1:3106828/100‑4 (MQ=255)
                    ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                 >  1:1321742/1‑100 (MQ=255)
                    ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                 <  1:300370/100‑1 (MQ=255)
                    ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATCCggg                                                                                 <  1:2569958/100‑1 (MQ=255)
                     cacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggcagaagg                                                                                <  1:1493493/100‑9 (MQ=38)
                      acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGt                                                                                >  2:1779315/1‑99 (MQ=255)
                       cGATAGAACGCAATAAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGTCGTTTGCCTTCTGCACTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                               >  1:1076375/1‑99 (MQ=255)
                        gATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCg                                                                             <  2:550555/100‑1 (MQ=255)
                              aCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                       >  1:4049756/1‑100 (MQ=255)
                               cGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                      >  2:1387996/1‑100 (MQ=255)
                                gCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                     <  1:2412609/100‑1 (MQ=255)
                                gCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                     <  1:3953642/100‑1 (MQ=255)
                                 caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                    >  2:2551418/1‑100 (MQ=255)
                                 caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                    <  1:3038847/100‑1 (MQ=255)
                                   atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCa                                                                  <  2:181821/100‑1 (MQ=255)
                                   atcaatGCCGCTAATGCCAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                    <  2:1947953/98‑1 (MQ=255)
                                    tcaatGCCGCTAATGCGAAAGTAAGGAGCGACGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCAt                                                                 >  2:160847/1‑100 (MQ=255)
                                      aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAc                                                               >  1:3688516/1‑100 (MQ=255)
                                      aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAc                                                               <  2:3517282/100‑1 (MQ=255)
                                           ngCTAATGCGAAAGTAAGGCGCGCCGGACGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAGCGTTg                                                          >  2:1520437/2‑100 (MQ=255)
                                           cGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                          <  1:725087/100‑1 (MQ=255)
                                              tAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgc                                                       <  2:2337734/100‑1 (MQ=255)
                                                   cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  >  2:818918/1‑100 (MQ=255)
                                                   cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  >  1:38329/1‑100 (MQ=255)
                                                   cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  <  1:1374515/100‑1 (MQ=255)
                                                     aaaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAg                                                <  2:135702/100‑1 (MQ=255)
                                                      aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTTTGCTCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  >  2:1619498/1‑97 (MQ=255)
                                                      aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGa                                               >  2:3625595/1‑100 (MQ=255)
                                                        gTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAca                                             <  2:3267247/100‑1 (MQ=255)
                                                          aaGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagc                                           <  1:451299/100‑1 (MQ=255)
                                                           aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                          >  1:2665775/1‑100 (MQ=255)
                                                            ggCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                         <  1:344749/100‑1 (MQ=255)
                                                             gcgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACagcagc                                        <  2:3114285/100‑1 (MQ=255)
                                                              cgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGCTAGACAGCAGCt                                       >  1:2890968/1‑100 (MQ=255)
                                                                cgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGa                                     >  1:1631799/1‑100 (MQ=255)
                                                                 gcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCACCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGAt                                    <  2:2784636/100‑1 (MQ=255)
                                                                 gcCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGAt                                    <  2:2784622/100‑1 (MQ=255)
                                                                  ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                   <  1:1520449/100‑1 (MQ=255)
                                                                     gAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCtt                                >  2:2684855/1‑100 (MQ=255)
                                                                       aGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTaa                              <  2:38329/100‑1 (MQ=255)
                                                                        gtgtGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAAt                             <  1:544869/100‑1 (MQ=255)
                                                                        gtgtGCCGGTTGCCTCCTCCCCTGCCAGCAGCTGTGTCAGGATGCGGGTGCGGAATTTGGTCATACCGTTGAGCGCGCTAGACAGCAGCTGATGCtt                                <  2:3356873/97‑1 (MQ=255)
                                                                          gtgCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGt                           >  1:3094686/1‑100 (MQ=255)
                                                                          gtgCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGt                           >  1:1191458/1‑100 (MQ=255)
                                                                           tgCCGTTTGCCTTCTGCTCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTa                          >  2:1897652/1‑100 (MQ=255)
                                                                            gCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTAc                         >  1:2569322/1‑100 (MQ=255)
                                                                             ccGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACg                        <  1:3396202/100‑1 (MQ=255)
                                                                               gtgtGCCGTCTGCCCTGCCAGCAGCTCTGGCAGGATGCGGGCGCGGAACTTTGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTa                          <  2:3714312/93‑1 (MQ=255)
                                                                                tttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGt                     <  1:2551432/100‑1 (MQ=255)
                                                                                    tctcgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                 <  1:678257/92‑1 (MQ=38)
                                                                                    ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                 <  1:672556/100‑1 (MQ=255)
                                                                                    ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                 <  1:953231/100‑1 (MQ=255)
                                                                                     cTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                >  1:618156/1‑100 (MQ=255)
                                                                                     cTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                 <  2:2514600/99‑1 (MQ=255)
                                                                                       cgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgaga              >  2:678251/6‑97 (MQ=38)
                                                                                       cgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaa              >  2:1909692/6‑100 (MQ=38)
                                                                                       cgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaa              >  1:262925/6‑100 (MQ=38)
                                                                                        ctgccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa             >  1:722554/1‑100 (MQ=255)
                                                                                         catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc            >  2:1595824/4‑100 (MQ=255)
                                                                                          gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGTAGCTGATGCTTAATGTACGGGTTACGGAAACg           <  1:3922202/100‑1 (MQ=255)
                                                                                          gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAACCg           >  2:3341606/1‑100 (MQ=255)
                                                                                          gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  1:1200710/1‑100 (MQ=255)
                                                                                          gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  2:1222552/1‑100 (MQ=255)
                                                                                          atcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATCGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  1:1061714/3‑100 (MQ=255)
                                                                                            cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc         >  1:1893485/1‑100 (MQ=255)
                                                                                            cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc         >  1:2538578/1‑100 (MQ=255)
                                                                                               gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGccc        >  2:493689/1‑98 (MQ=255)
                                                                                               gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg      <  1:1865324/100‑1 (MQ=255)
                                                                                               gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg      <  1:2678556/100‑1 (MQ=255)
                                                                                                  agcagcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGa   >  2:1907172/1‑100 (MQ=255)
                                                                                                   gcagcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc  <  2:2616630/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
ATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAC  >  NC_000913/1609456‑1609654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: