Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,165,590 Δ1 bp 100% intergenic (+69/‑78) yegP → / → yegQ UPF0339 family protein/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,5880C.59.3% 73.9 / 283.0 140intergenic (+67/‑80)yegP/yegQUPF0339 family protein/putative peptidase
Reads supporting (aligned to +/- strand):  ref base C (22/35);  new base . (35/48);  total (57/83)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 151 total aligned reads displayed.

GGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCC  >  NC_000913/2165489‑2165686
                                                                                                   |                                                                                                  
 gCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgcc                                                                                                   >  1:3318434/1‑100 (MQ=255)
  cacaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgccc                                                                                                  >  1:1904809/1‑100 (MQ=255)
  cacaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgccc                                                                                                  >  1:3593024/1‑100 (MQ=255)
   acaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgcccg                                                                                                 <  1:1288873/100‑1 (MQ=255)
   acaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGGGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgccgc                                                                                                 >  2:1122564/1‑98 (MQ=255)
    caAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTGGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgccgc                                                                                                 >  2:2666683/1‑97 (MQ=255)
        ccAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTa                                                                                           >  2:2790622/1‑100 (MQ=255)
         cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTa                                                                                           <  1:1582142/100‑1 (MQ=255)
         cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTaa                                                                                          >  2:1464296/1‑100 (MQ=255)
           gACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTaaa                                                                                         >  1:3949231/1‑100 (MQ=255)
           gACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAg                                                                                        >  2:3055689/1‑100 (MQ=255)
            aCGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGt                                                                                       <  2:3850568/100‑1 (MQ=255)
             cGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTg                                                                                      >  1:2588813/1‑100 (MQ=255)
              ggTGAAAGTCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTgg                                                                                     <  2:1226877/100‑1 (MQ=255)
              ggTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTgg                                                                                     >  1:2814566/1‑100 (MQ=255)
               gTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTggg                                                                                    <  2:3952245/100‑1 (MQ=255)
                tGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTggg                                                                                    <  1:1415817/100‑1 (MQ=255)
                tGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTgggg                                                                                   <  1:1977238/100‑1 (MQ=255)
                 gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTgggg                                                                                   <  2:1282798/100‑1 (MQ=255)
                 gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTggggg                                                                                  <  1:3241390/100‑1 (MQ=255)
                  aaaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGc                                                                                 >  1:259234/1‑100 (MQ=255)
                  aaaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGc                                                                                 <  2:4133638/100‑1 (MQ=255)
                   aaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGc                                                                                 <  1:3229381/100‑1 (MQ=255)
                   aaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGc                                                                                 <  1:1202436/100‑1 (MQ=255)
                    aGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCAc                                                                               <  2:3659275/100‑1 (MQ=255)
                        aaTACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTccc                                                                           <  2:1808208/100‑1 (MQ=255)
                        aaTACCTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCGCCCACAGCGCTACAAGGCCCGCCCTTAAAGTGGGGGc                                                                                 <  2:1031430/95‑1 (MQ=255)
                         aTACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTcccc                                                                          <  1:84998/100‑1 (MQ=255)
                          tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTcccc                                                                          <  2:768698/100‑1 (MQ=255)
                          tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTcccc                                                                          <  2:775955/100‑1 (MQ=255)
                            cGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTa                                                                        <  1:3215032/100‑1 (MQ=255)
                               aaTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAAcc                                                                     <  1:4039295/99‑1 (MQ=255)
                               aaTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCg                                                                    >  1:2666623/1‑100 (MQ=255)
                               aaTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCg                                                                    >  1:3615355/1‑100 (MQ=255)
                                aTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCg                                                                    >  1:2488826/1‑100 (MQ=255)
                                aTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGc                                                                   <  1:469936/100‑1 (MQ=255)
                                  tACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCt                                                                  <  2:3571680/100‑1 (MQ=255)
                                  tACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCt                                                                  <  1:1651751/100‑1 (MQ=255)
                                  tACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCtt                                                                 >  1:920562/1‑100 (MQ=255)
                                   aCGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtc                                                                >  1:1996223/1‑100 (MQ=255)
                                    cGATGCCGGGCGTTGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtc                                                                <  2:1359096/100‑1 (MQ=255)
                                    cGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtca                                                               >  2:3429901/1‑100 (MQ=255)
                                     gATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtca                                                               <  1:539874/100‑1 (MQ=255)
                                      aTGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcatc                                                             <  2:3298291/100‑1 (MQ=255)
                                       tGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcatca                                                            <  1:3159681/100‑1 (MQ=255)
                                       tGCCGGGCGTGGGTTGTGGGCGGCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcatc                                                             <  1:1850806/100‑1 (MQ=255)
                                        gCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAg                                                           <  1:844185/100‑1 (MQ=255)
                                         ccGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAgg                                                          >  2:2662384/1‑100 (MQ=255)
                                          cGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACCGCGCTACACTGCCGCCCTTAAAGTGGGTGCACTCCCCTAACCGCTtcacctga                                                          <  2:3491553/99‑6 (MQ=255)
                                             gcctGGGTTGTTGGCGTCCGGCGCTTTACCAGATCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGa                                                       <  2:1841753/96‑1 (MQ=255)
                                                 gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCg                                                   <  2:241774/100‑1 (MQ=255)
                                                 gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCg                                                   <  2:4071509/100‑1 (MQ=255)
                                                  ggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                 >  1:1127915/1‑100 (MQ=255)
                                                  ggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                 >  1:1079500/1‑100 (MQ=255)
                                                   gttgttGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                 >  1:1814943/1‑100 (MQ=255)
                                                    ttgttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATc                                               <  1:994330/100‑1 (MQ=255)
                                                    ttgttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATc                                               >  1:2355347/1‑100 (MQ=255)
                                                    ttggtgtcttCCGGTGCTTTACCAGAGCCCCGGACAGCTCTACAATGCCGCGCTTAAAGTTGGGGTACTCCCCTATCCGCTTCATCATGTGAAGCGGATc                                               <  2:394952/91‑1 (MQ=255)
                                                     tgttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATc                                               <  2:4046790/100‑1 (MQ=255)
                                                     tgttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTCAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGCAGCGGATc                                               >  2:2872903/1‑99 (MQ=255)
                                                     tggtgGCGTGCGGCGCTTTACCAGGGCCCCCCACAGCGCTACAATGCCCGCCGTTAAAGTGGGTGCACTCCCCTATCCGCTTCATCCGGTGAAGCGGATc                                               <  2:2561159/97‑1 (MQ=255)
                                                      gttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCACCTGATGAAGCggttagg                                             <  2:4134550/100‑6 (MQ=38)
                                                         ggCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc                                           <  2:3331615/100‑1 (MQ=255)
                                                         ggCGTCAGGCTCTTTCCCAGCGCGCCCCACTGCCCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAcc                                          <  2:3660060/100‑1 (MQ=255)
                                                           cGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTg                                        <  1:2721838/100‑1 (MQ=255)
                                                            gTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGt                                       >  1:3909160/1‑100 (MQ=255)
                                                             tCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtc                                      <  1:1843134/100‑1 (MQ=255)
                                                              ccGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTATCCGCTTCATCAGGTGAAGCGGATCTGACCTGtca                                     >  2:2179532/1‑100 (MQ=255)
                                                                ggCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcat                                    >  1:1559327/1‑100 (MQ=255)
                                                                 gcgcTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatc                                   <  1:4104712/100‑1 (MQ=255)
                                                                  cgcTTTACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg                                 <  2:3297474/100‑1 (MQ=255)
                                                                    cTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGa                                >  1:3406708/1‑100 (MQ=255)
                                                                       tACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg                             >  2:2727049/1‑100 (MQ=255)
                                                                       tACCAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                            >  1:3431261/1‑100 (MQ=255)
                                                                         ccAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgaga                          <  2:1672394/100‑1 (MQ=255)
                                                                         ccAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGGGGGGGCACGCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgag                           <  1:1056807/99‑1 (MQ=255)
                                                                          cAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagag                         >  1:2323908/1‑100 (MQ=255)
                                                                          cAGAGCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagag                         <  2:1996210/100‑1 (MQ=255)
                                                                           agagCGCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCCGGTGAAGCGGATCTGACCTGTCATCAGAACgagaga                        >  2:2158022/1‑100 (MQ=255)
                                                                             agCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt                      <  2:3470486/100‑1 (MQ=255)
                                                                              gCCCCCCACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAtt                     >  1:2658637/1‑100 (MQ=255)
                                                                                cccccACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTAt                   >  1:279041/1‑100 (MQ=255)
                                                                                 ccccACAGTGCTACCACTCCCTTCCTTAAAGGTGGAGCACTCCCCTAACCTCTTCATCAAGTGTAGCAGATCTGACCTGTCATCAGAACgagag                         <  2:3931502/94‑1 (MQ=255)
                                                                                  cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATg                  >  2:2351767/1‑100 (MQ=255)
                                                                                   ccACAGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGtt                <  1:2165605/100‑1 (MQ=255)
                                                                                    cacaGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGtt                <  1:239699/100‑1 (MQ=255)
                                                                                    cacaGCGCTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGttt               <  1:2928438/100‑1 (MQ=255)
                                                                                      caGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTa              >  2:3155845/1‑100 (MQ=255)
                                                                                         cgcTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAcc          <  1:1553466/100‑1 (MQ=255)
                                                                                         cgcTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAcc          <  2:1340175/100‑1 (MQ=255)
                                                                                          gcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAcc          >  2:2921181/1‑100 (MQ=255)
                                                                                          gcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAcc          >  1:2193044/1‑100 (MQ=255)
                                                                                           cTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCg         >  1:3141495/1‑100 (MQ=255)
                                                                                           cTACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCAGCAGAACGAGAGAATTATGTTTAAACCgg        <  2:808580/100‑1 (MQ=255)
                                                                                            tACAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGa       <  1:898532/100‑1 (MQ=255)
                                                                                             aCAATGCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCGTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGa       <  2:3811372/99‑1 (MQ=255)
                                                                                              cAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCCTCAGCTGAACCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAAcacgaa      >  2:2281040/1‑95 (MQ=255)
                                                                                                aTGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACt    >  2:3920888/1‑100 (MQ=255)
                                                                                                 tGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTc   <  2:1603849/100‑1 (MQ=255)
                                                                                                  gcccgcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTcc  <  2:3593001/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCC  >  NC_000913/2165489‑2165686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: