Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,874 Δ1 bp 15.8% intergenic (‑137/+31) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8730C.15.8% 342.4 / 65.6 120intergenic (‑136/+32)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (43/58);  new base . (8/11);  total (51/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 122 total aligned reads displayed.

TAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCT  >  NC_000913/2596775‑2596972
                                                                                                  |                                                                                                    
tAGGTGGTTCAGAGGAAGATTACCACAGAAAAACGGGATGAAATGGAGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGcc                                                                                                     <  1:2755953/100‑1 (MQ=255)
 aGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCa                                                                                                    <  1:2765123/100‑1 (MQ=255)
  ggtggtTCAGAGGAAGATTACAACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAg                                                                                                   >  1:2460834/1‑100 (MQ=255)
   gtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                  <  2:1155748/100‑1 (MQ=255)
     ggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                               >  1:1369555/1‑100 (MQ=255)
      gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                              <  2:671450/100‑1 (MQ=255)
      gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                              >  2:389842/1‑100 (MQ=255)
       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCa                                                                                             >  1:4012353/1‑100 (MQ=255)
         cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                           <  1:3372287/100‑1 (MQ=255)
          agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATACCGAGAGCGGTTGTCTTGCCGTGCCAGCt                                                                                                 >  2:4008988/1‑94 (MQ=255)
          agagGAAGAGTCCCACAGGAAAGCGGGAGGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                           <  2:312324/99‑1 (MQ=255)
           gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                         >  1:2450010/1‑100 (MQ=255)
           gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                         >  1:1598223/1‑100 (MQ=255)
           gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                         >  2:4111737/1‑100 (MQ=255)
            agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTggtacggca                                                                                       <  2:3202192/100‑9 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCaa                                                                                       >  1:1962467/1‑100 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCaa                                                                                       <  2:2284016/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                       <  1:3954041/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                       <  1:303703/100‑1 (MQ=255)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                      <  2:2818642/100‑1 (MQ=39)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                      <  1:1373591/100‑1 (MQ=39)
                  aTTACCACAGTAAAGCGGGATGAAATGGCGGAGTAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAgacaa                                                                                  <  2:3079042/100‑4 (MQ=37)
                  aTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                  <  2:1422764/100‑1 (MQ=255)
                    tACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAgacaacc                                                                                <  1:4085516/100‑6 (MQ=38)
                     aCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAtgatga                                                                               <  2:900973/100‑1 (MQ=255)
                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                              >  2:2980374/1‑100 (MQ=255)
                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                              <  1:1541919/100‑1 (MQ=255)
                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATGCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                              <  2:3286752/100‑1 (MQ=255)
                        acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                            <  2:2319215/100‑1 (MQ=255)
                            gAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                            <  2:3442211/96‑1 (MQ=255)
                            gAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaa                                                                        <  2:982567/100‑1 (MQ=255)
                              aaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                      <  2:2321515/100‑1 (MQ=255)
                               aGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                     <  1:1670894/100‑1 (MQ=255)
                                  gtgttGAAACGGCGCAAGAAAGTGCGCCCAATACATATATTCCAACAGCAGAAAACTTTCATAGCCACCTGCACGGCAAGATGATGAACAGAAAAATc                                                                    <  2:3842819/94‑1 (MQ=255)
                                  gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATc                                                                    <  2:808416/98‑1 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCATTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACCGCAAGATGTTGAACAGAAAAATCAg                                                                  >  2:2967746/1‑99 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                 >  2:609992/1‑100 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                 <  2:1782950/100‑1 (MQ=255)
                                    gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                >  2:2123658/1‑100 (MQ=255)
                                    gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                >  2:3845826/1‑100 (MQ=255)
                                    gATGAAATGGATGATGAATGATCGCTCAATGCATATACTCCGAGAGCTGTTGTCTTGACGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAg                                                                  <  2:3770248/98‑1 (MQ=255)
                                         aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCt                                                           >  1:2105899/1‑100 (MQ=255)
                                         aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCt                                                           <  1:1781411/100‑1 (MQ=255)
                                          aTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg                                                          <  2:1369546/100‑1 (MQ=255)
                                           tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGt                                                         >  2:2424265/1‑100 (MQ=255)
                                            ggCGGGGGAATGAGCGTTCAATGCATATATGCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                        <  2:3546406/100‑1 (MQ=255)
                                             gCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                       >  1:3991109/1‑100 (MQ=255)
                                              cGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTaca                                                      >  1:3596902/1‑100 (MQ=255)
                                                  ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                  >  2:1131190/1‑100 (MQ=255)
                                                    aaTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAgg                                                <  1:2767857/100‑1 (MQ=255)
                                                     aTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTCGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggc                                               <  2:4103292/100‑1 (MQ=255)
                                                       gAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggcgg                                             >  1:4104667/1‑100 (MQ=255)
                                                        aGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAACAATCAGTCCAGCTGTACACCCAggcggc                                            >  2:405365/1‑100 (MQ=255)
                                                          cGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                          <  2:3497952/100‑1 (MQ=255)
                                                            ttCAATGCATATATTCCGAGAGCGGGTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                         <  2:2119529/99‑1 (MQ=255)
                                                               aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                     <  1:4009014/100‑1 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                     >  2:1491075/1‑98 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                   >  1:4101242/1‑100 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                   >  1:3911384/1‑100 (MQ=255)
                                                                  gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACATAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                  <  2:2259981/100‑1 (MQ=255)
                                                                  gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                  <  2:905289/100‑1 (MQ=255)
                                                                   cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                 <  1:3852959/100‑1 (MQ=255)
                                                                   cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                 >  1:593488/1‑100 (MQ=255)
                                                                    atatatTCCGAGAGCGGTTGTCTTGCCGTGCCCGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCAGCGGGCGACGGCtt                                >  1:1191729/1‑100 (MQ=255)
                                                                    atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCtt                                <  1:3086132/100‑1 (MQ=255)
                                                                    atatacTCCGAGAGCGGTTGTCTTGCAGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCtt                                <  2:1934799/100‑1 (MQ=255)
                                                                     tatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACATCCAGGCGGCGGGCGACGGCTTc                               <  2:2397303/100‑1 (MQ=255)
                                                                        atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCCAGATGATGAACAGAAAACTCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCa                              >  2:3482138/1‑98 (MQ=255)
                                                                        atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                            >  1:1480350/1‑100 (MQ=255)
                                                                           ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                         >  1:353403/1‑100 (MQ=255)
                                                                           ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                         >  1:185462/1‑100 (MQ=255)
                                                                           ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                         <  1:45794/100‑1 (MQ=255)
                                                                           ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                         >  2:180847/1‑100 (MQ=255)
                                                                            cGAGAGCGGTTGTCTTGCCGTGCCCGATGCACGGCAAGATGATGAACAGAAAACTCAGTCCCGCTGTACACCCACGCGGCGGGCGACCGCTTCATa                            >  2:1937782/1‑96 (MQ=255)
                                                                            cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt                        <  1:3125359/100‑1 (MQ=255)
                                                                             gagaGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCtt                       >  2:2983361/1‑100 (MQ=255)
                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                      <  1:3126502/100‑1 (MQ=255)
                                                                                   ggTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCa                 <  2:623136/100‑1 (MQ=255)
                                                                                    gTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg                <  1:3996745/100‑1 (MQ=255)
                                                                                    atcatCTTGCCGTGCAGCTGGCACGGCAAGATGATGTACAGAAAAATCAGTCCCGCTGTACACCCAGGCGGCGGGCGACGGCTTCATCGGCTTCGATCa                 >  2:1896401/5‑99 (MQ=25)
                                                                                     ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCatg               <  1:2790746/100‑3 (MQ=255)
                                                                                     ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg               <  2:328248/100‑1 (MQ=255)
                                                                                     ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg               <  1:1481684/100‑1 (MQ=255)
                                                                                      tGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc              <  1:1544985/100‑1 (MQ=255)
                                                                                      tGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc              >  1:2258994/1‑100 (MQ=255)
                                                                                      tGTCTTGCCGTGCCAGCTCCACGGCAAGAGGATGAACAGAAAGATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAt                   <  2:679220/95‑1 (MQ=255)
                                                                                       gTCTTGCAGTGCCAGCTGCACGTCTAGATGATGAACAGAAAAAGCACTCCAGCTGTTCACCCAGACTGCGGGCGACGGCTTTTTAGGCTTCGATCAggtc             <  2:4057725/100‑3 (MQ=255)
                                                                                       atCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGCCGCCCCCATCGGCGTCGATCACGcc             >  1:3736486/2‑100 (MQ=25)
                                                                                       atCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGcc             >  1:4065558/2‑100 (MQ=39)
                                                                                          ttGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAcc          >  1:1753967/1‑100 (MQ=255)
                                                                                          ttGCCGTGCAGCTGGCACGGCACGATGCTGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCTGGCCAcc          >  2:1447391/1‑100 (MQ=255)
                                                                                           tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg         >  1:2969488/1‑100 (MQ=255)
                                                                                           tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg         >  1:1307454/1‑100 (MQ=255)
                                                                                           tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCGGTACACCCAGGCGGCGGGCGACTGTTTCATAGACTTCGATCAGGCCACCg         <  2:1331228/100‑1 (MQ=255)
                                                                                           tGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCAAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg         >  1:63719/1‑100 (MQ=255)
                                                                                           tGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAACCAGTACAGCAGTACACCCAGGCGGCGCGCGACGGCGTAATCGGCTTCGCACAGGCCACCg         >  2:853191/1‑100 (MQ=255)
                                                                                              cGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAgg      <  1:3674851/100‑1 (MQ=255)
                                                                                               gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGc     <  1:3419854/100‑1 (MQ=255)
                                                                                               gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCACGCCACCGAGGc     >  2:2364798/1‑100 (MQ=255)
                                                                                                  ccAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctct  >  1:1260836/1‑100 (MQ=255)
                                                                                                  |                                                                                                    
TAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCT  >  NC_000913/2596775‑2596972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: