Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,066,319 Δ1 bp 19.3% coding (855/897 nt) ygfI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,066,3190C.19.3% 544.3 / 137.2 192coding (855/897 nt)ygfIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (83/71);  new base . (18/19);  total (102/90)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

TCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTC  >  NC_000913/3066220‑3066418
                                                                                                     |                                                                                                   
 cTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCa                                                                                                    >  1:1027039/1‑100 (MQ=255)
    aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCg                                                                                                 <  2:760567/100‑1 (MQ=255)
    aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCATATTGCTGAAGATCGt                                                                                                <  1:3764666/100‑1 (MQ=255)
     gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGt                                                                                                <  2:668686/100‑1 (MQ=25)
      ccACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTg                                                                                               <  1:854942/100‑1 (MQ=255)
       cACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                              >  1:4008215/1‑100 (MQ=255)
        aCGTCTTCGACTTACTCCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGc                                                                                             <  2:3683951/100‑1 (MQ=255)
        aCGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGc                                                                                             <  1:1727084/100‑1 (MQ=255)
        aCGTCTGCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                              <  2:3271963/99‑1 (MQ=255)
         cGTCTTCGACTTACATCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  1:4078922/100‑1 (MQ=21)
         cGTCTTCGACTTACACCGTACTTTAATTTCAACCGGCTTGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  2:2730161/100‑1 (MQ=18)
         cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  1:1884120/100‑1 (MQ=21)
         cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  1:2097601/100‑1 (MQ=21)
         cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  1:2152544/100‑1 (MQ=21)
          ttataccaCTTACACCATAATTTACTGGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCAACGATGCTACTGCATATTGCTGAAGATCGTGACgg                                                                                           <  2:4063576/93‑1 (MQ=12)
          gTCTTCGACTTACACCGTAATTTAATTGCAGCCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATGCTTCAGCATATTGCTGAAGATCGTGACg                                                                                            <  2:543589/99‑1 (MQ=21)
          gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                           <  2:2937083/100‑1 (MQ=255)
          gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                           >  1:294614/1‑100 (MQ=255)
          gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                           <  1:131721/100‑1 (MQ=255)
          gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACGCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACgg                                                                                           <  2:1264173/100‑1 (MQ=21)
           tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGt                                                                                          >  1:3283264/1‑100 (MQ=255)
            cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGta                                                                                         <  1:2025460/100‑1 (MQ=255)
            cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGta                                                                                         <  2:1898727/100‑1 (MQ=255)
            cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGta                                                                                         <  1:3080500/100‑1 (MQ=255)
            cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGta                                                                                         >  2:2614456/1‑100 (MQ=255)
             ttCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtat                                                                                        <  1:2344680/100‑1 (MQ=255)
              tCGACTTACACCGTAATTTAATTGCAACCGGCTCGGTGTTGGGTTAATGACTCGTTACGTCAAGATCTTCAGCAATATTGTGAAGACTCGTGTGGGtat                                                                                        >  2:1920790/1‑99 (MQ=255)
                gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                     >  2:554838/1‑100 (MQ=255)
                gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                     >  2:3185112/1‑100 (MQ=255)
                gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                     >  2:1297846/1‑100 (MQ=255)
                gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                     <  2:2079165/100‑1 (MQ=255)
                  cTTACACCGTGATTTCATTGCACCCCGCTCGATGCAGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATATTGCTGAAGATCGTGACggg                                                                                          <  2:223612/93‑2 (MQ=9)
                  cTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAAc                                                                                     >  2:858951/1‑98 (MQ=255)
                  cTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                                                   >  1:606856/1‑100 (MQ=255)
                    tACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGATGAAGCATCGTGGCGGTATAACgatg                                                                                 >  1:1914640/1‑100 (MQ=255)
                        ccGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGAttt                                                                             >  1:2866943/1‑100 (MQ=255)
                         cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGAttt                                                                             >  2:3269239/1‑99 (MQ=255)
                          gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCt                                                                           <  2:1034246/100‑1 (MQ=255)
                          gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCt                                                                           <  2:3286348/100‑1 (MQ=255)
                              tttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAtt                                                                       >  2:1341103/1‑100 (MQ=255)
                               ttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGTTGATTTCTGATTg                                                                      <  1:2808052/100‑1 (MQ=255)
                                  attGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTGTAACGATGATTTCTGATTGAAt                                                                   >  2:3071404/1‑100 (MQ=255)
                                  attGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                                   >  1:2258076/1‑100 (MQ=255)
                                     gCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                                >  1:3140840/1‑100 (MQ=255)
                                      cAACCGGCTCGATGCTGGGTTAATGACTCGTCGCGTCACGATCTTCAGCAATATGGTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                                               >  1:3927616/1‑100 (MQ=255)
                                       aaCCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                              >  1:3576182/1‑100 (MQ=255)
                                            gCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                         >  1:1716766/1‑100 (MQ=255)
                                                 aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGc                                                     <  2:902965/99‑1 (MQ=255)
                                                 aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                                    <  1:2597810/100‑1 (MQ=255)
                                                 aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                                    >  1:3527278/1‑100 (MQ=255)
                                                    cTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATGTCTGATTGAATAAAGATATTCCTGCatat                                                 >  2:1739484/1‑100 (MQ=255)
                                                          tAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATc                                            >  1:478580/1‑99 (MQ=255)
                                                             tGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGcc                                        <  1:1579925/100‑1 (MQ=255)
                                                               aCTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCtg                                      <  2:3708649/100‑1 (MQ=255)
                                                                  cGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGc                                   >  1:1061413/1‑100 (MQ=255)
                                                                    tCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg                                 <  1:333174/100‑1 (MQ=255)
                                                                     cccGTCACGATCTTCAGCAATATGATGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATAGATCGGCCtgtg                                    >  2:19607/1‑96 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGt                                >  1:77580/1‑99 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                               <  1:1021072/100‑1 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                               >  1:2417157/1‑100 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                               >  1:2208635/1‑100 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTg                               >  2:1024689/1‑100 (MQ=255)
                                                                      ccGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTCCGGTg                               >  2:3499516/1‑100 (MQ=255)
                                                                       cGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGc                              <  1:3462728/100‑1 (MQ=255)
                                                                        gccACGATGCTTCAGCATATTGCTGAAGATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCa                             >  1:3811469/3‑100 (MQ=25)
                                                                         tCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt                            >  1:438568/1‑100 (MQ=255)
                                                                          cACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                           >  2:4041543/1‑100 (MQ=255)
                                                                            cgatgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaa                         >  1:2636645/6‑100 (MQ=25)
                                                                            cgatgcTTCAGCATATTGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaa                         >  1:1173122/6‑100 (MQ=25)
                                                                            cGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaa                         >  2:2115342/1‑100 (MQ=255)
                                                                            cGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCa                          >  2:43143/1‑99 (MQ=255)
                                                                              aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                       >  1:1136233/1‑100 (MQ=255)
                                                                              aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                       <  1:3370521/100‑1 (MQ=255)
                                                                              aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                       <  1:2749913/100‑1 (MQ=255)
                                                                              aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                       <  2:2835550/100‑1 (MQ=255)
                                                                              aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCaaaa                       <  1:3681138/100‑1 (MQ=255)
                                                                                  ttCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAatat                    >  2:3952308/1‑100 (MQ=255)
                                                                                  ttCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAatat                    >  2:1988111/1‑100 (MQ=255)
                                                                                   tCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAtt                   <  1:3175544/100‑1 (MQ=255)
                                                                                   tCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAtt                   >  2:1121422/1‑100 (MQ=255)
                                                                                   tCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAtt                   >  1:944020/1‑100 (MQ=255)
                                                                                     cagcatattGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATAttag                 >  2:3767152/9‑98 (MQ=38)
                                                                                      gCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCa                >  1:3654058/1‑100 (MQ=255)
                                                                                       cAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAg               <  1:677681/100‑1 (MQ=255)
                                                                                       cAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAg               <  1:372427/100‑1 (MQ=255)
                                                                                         atattGCTGAAGATCGTGACGGTATAACGATGATTTCTGATTGAATAAAGATATCCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCg             >  1:2698281/5‑100 (MQ=255)
                                                                                           atGCTGCAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCCGCGt            <  2:138944/99‑1 (MQ=255)
                                                                                             tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTg          <  2:3561580/100‑1 (MQ=255)
                                                                                             tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTg          >  1:1448616/1‑100 (MQ=255)
                                                                                             tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTg          >  1:1472557/1‑100 (MQ=255)
                                                                                              gCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGc         >  1:3349511/1‑100 (MQ=255)
                                                                                               cTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGcc        >  2:2186158/1‑100 (MQ=255)
                                                                                                tGAAGCATCGTGGCGGTGTAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCg       <  2:2529457/100‑1 (MQ=255)
                                                                                                 gAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCgg      >  1:2477566/1‑100 (MQ=255)
                                                                                                 gAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCgg      <  2:3842633/100‑1 (MQ=255)
                                                                                                   aGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGa     >  1:903651/1‑99 (MQ=255)
                                                                                                   aGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAt    <  2:474561/100‑1 (MQ=255)
                                                                                                   aGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAt    <  1:543972/100‑1 (MQ=255)
                                                                                                   aGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGAt    <  1:514731/100‑1 (MQ=255)
                                                                                                     cATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTc  <  1:2045378/100‑1 (MQ=255)
                                                                                                     |                                                                                                   
TCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTC  >  NC_000913/3066220‑3066418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: