Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,642,062:1 +T 100% coding (208/213 nt) cspF → Qin prophage; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,642,0591.T57.4% 134.5 / 113.1 68coding (205/213 nt)cspFQin prophage; cold shock protein
Reads supporting (aligned to +/- strand):  ref base . (16/13);  new base T (18/21);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTG  >  NC_000913/1641961‑1642158
                                                                                                   |                                                                                                    
cgcTCAATCTCCGCGACGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCt                                                                                                     <  2:2418331/100‑1 (MQ=255)
 gcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCtt                                                                                                   <  1:589738/100‑1 (MQ=255)
 gcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCtt                                                                                                   >  1:3599138/1‑100 (MQ=255)
   tCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTc                                                                                                 <  1:1423232/100‑1 (MQ=255)
     aaTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttca                                                                                               <  2:2965907/100‑4 (MQ=255)
      aTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttcat                                                                                              <  2:350506/100‑5 (MQ=255)
      aTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttcat                                                                                              >  1:2689006/1‑96 (MQ=255)
      aTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATg                                                                                              <  2:2921116/100‑1 (MQ=255)
      aTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACAttttcat                                                                                              >  2:4000845/1‑96 (MQ=255)
         tcCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGc                                                                                           >  2:2545864/1‑100 (MQ=255)
          ccGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGc                                                                                           >  1:3331814/1‑100 (MQ=255)
          ccGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCt                                                                                          <  1:2667844/100‑1 (MQ=255)
           cgcgATGTAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGTCGGATAAATGGTCTGCGTGGCCCTTCAGTTGCCAATGTTTACCttttcat                                                                                              <  2:511549/95‑5 (MQ=255)
                 gCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTaa                                                                                   <  1:1647797/100‑1 (MQ=255)
                   agaagaAATTACCACCGGATTCCGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAg                                                                                 >  2:2613872/1‑100 (MQ=255)
                   agaagaAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAAGGTTTACCTTTCATGAGCTATATTAAAg                                                                                 >  2:1170138/1‑100 (MQ=255)
                      agaaATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCtt                                                                              >  2:2915232/1‑100 (MQ=255)
                        aaaTTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTa                                                                            <  2:3643684/100‑1 (MQ=255)
                        aaaTTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTa                                                                            <  1:1097543/100‑1 (MQ=255)
                         aaTTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTa                                                                            >  2:656110/1‑100 (MQ=255)
                          aTTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAAt                                                                          <  2:3009331/100‑1 (MQ=255)
                              ccaccGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTc                                                                       >  1:2526674/1‑100 (MQ=255)
                              ccaccGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTc                                                                       >  1:1939563/1‑100 (MQ=255)
                              ccaccGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCa                                                                      >  1:2454004/1‑100 (MQ=255)
                                accGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAg                                                                     >  1:2240592/1‑100 (MQ=255)
                                 ccGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAgg                                                                    <  2:707106/100‑1 (MQ=255)
                                  cGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGc                                                                   <  2:1324274/100‑1 (MQ=255)
                                   ggATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGcc                                                                  <  1:1457856/100‑1 (MQ=255)
                                   ggATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGcc                                                                  >  1:1862834/1‑100 (MQ=255)
                                   ggATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGcc                                                                  >  1:2615605/1‑100 (MQ=255)
                                   ggATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGccc                                                                 >  1:1479561/1‑100 (MQ=255)
                                        aCGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATc                                                             >  1:2503402/1‑100 (MQ=255)
                                          gcgTGTAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATc                                                             <  1:19648/98‑1 (MQ=255)
                                          gcgTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCgg                                                           >  1:3277300/1‑100 (MQ=255)
                                             tGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGa                                                          <  2:3743070/98‑1 (MQ=255)
                                             tGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATc                                                        <  2:3137456/100‑1 (MQ=255)
                                             tGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATca                                                       >  1:3764707/1‑100 (MQ=255)
                                              ggAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATc                                                        <  2:3793874/99‑1 (MQ=255)
                                               gAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATcac                                                      <  1:2698638/100‑1 (MQ=255)
                                                 aTTTTGCTGGATAAATGGTCTGCGTGGCCCTTCCGCTGCCAATGTTTGCCTTTCATGAGCTATATTATAGCTTGAATGGCAGGCCCCAACGGATcac                                                      <  2:2705901/97‑1 (MQ=255)
                                                 aTTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATcacaca                                                   >  1:651069/1‑100 (MQ=255)
                                                      gCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGgag                                              >  1:362639/1‑100 (MQ=255)
                                                      gCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGgag                                              <  1:3920569/100‑1 (MQ=255)
                                                       ccGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGgaga                                             >  1:2585588/1‑100 (MQ=255)
                                                          gATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGt                                           <  2:3568830/100‑1 (MQ=255)
                                                            tAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGttt                                         <  1:662632/100‑1 (MQ=255)
                                                               aTGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCATGCCCCATCGGATCACACATGGAGAGTTTTTa                                      <  1:768197/100‑1 (MQ=255)
                                                               aTGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTAt                                     <  1:3391758/100‑1 (MQ=255)
                                                                tGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTGAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTAt                                     <  2:3050136/100‑1 (MQ=255)
                                                                tGGTCTGCGTGGCCCTTCAGCTGCCAATGTGTACCTTTTCATGAGCTATAATAAAGCTTTAATTTCAGGCCCCATCGGCTCACACACGGAGAGTTTTTAt                                     <  2:4083437/100‑1 (MQ=255)
                                                                 ggTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATg                                    <  1:3388500/100‑1 (MQ=255)
                                                                 ggTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATg                                    <  1:2701803/100‑1 (MQ=255)
                                                                  atCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGa                                   >  2:3376388/2‑100 (MQ=255)
                                                                       cGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAAcc                             <  2:1489795/100‑1 (MQ=255)
                                                                         tGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAAccc                            <  2:2503388/100‑1 (MQ=255)
                                                                            cacatCAGCAGCCAAAATTTACGTATTCATGAGCAATATTGAAGCTATAATGTCAAGCCCCATAGGGTCAGAGATGGAGAGTTTTTATGAAGAAcggcg                          >  2:2404281/5‑95 (MQ=255)
                                                                             ccTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtc                        >  1:122092/1‑100 (MQ=255)
                                                                             ccTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtc                        >  2:1647737/1‑100 (MQ=255)
                                                                             ccTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtc                        <  1:3872319/100‑1 (MQ=255)
                                                                              cTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtct                       <  1:1954724/100‑1 (MQ=255)
                                                                              cTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtct                       >  1:3274452/1‑100 (MQ=255)
                                                                              cTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATGAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtct                       >  2:344239/1‑100 (MQ=255)
                                                                                  aGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtctgtct                   >  1:1711524/1‑100 (MQ=255)
                                                                                   gCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGTATAACCCCGTCTGTCTtg                 <  2:3876062/100‑1 (MQ=255)
                                                                                    cTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtg                 <  1:1496758/100‑1 (MQ=255)
                                                                                    cTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtga                >  2:3379798/1‑100 (MQ=255)
                                                                                     tGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtga                <  2:1504300/100‑1 (MQ=255)
                                                                                      gCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgat               >  2:3571806/1‑100 (MQ=255)
                                                                                      gCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCACTCCTCATCGCATCACACATGTAGTGTTTTTATGACTACACCCgtct                       >  2:3584031/1‑91 (MQ=255)
                                                                                       ccAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatg              >  2:1875428/1‑100 (MQ=255)
                                                                                       ccAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatga             >  1:602952/1‑100 (MQ=255)
                                                                                        cAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGAc            >  2:1135947/1‑100 (MQ=255)
                                                                                         aaTGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGAc            >  2:1722297/1‑100 (MQ=255)
                                                                                            gTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGt        >  2:386373/1‑100 (MQ=255)
                                                                                              ttACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGtt       <  2:3042489/100‑1 (MQ=255)
                                                                                              ttACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTg      <  2:3698244/100‑1 (MQ=255)
                                                                                                  cTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTg  >  1:4022771/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
CGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTG  >  NC_000913/1641961‑1642158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: