Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,712,306:1 (T)7→8 100% intergenic (+148/‑463) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,2991.T90.6% 304.3 / 24.5 96intergenic (+141/‑470)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base . (6/3);  new base T (46/41);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTT  >  NC_000913/1712202‑1712393
                                                                                                   |                                                                                               
aTCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGtt                                                                                               >  2:3679542/1‑100 (MQ=255)
 tCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGttt                                                                                              >  1:3492563/1‑100 (MQ=255)
  ccTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGtttt                                                                                             <  1:373789/100‑1 (MQ=255)
      ttttAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGtttttttt                                                                                        >  1:1762325/1‑99 (MQ=255)
      ttttAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGAGGATAACCAAAAATGCAGGCTAATTGtttttttt                                                                                        >  2:3260830/1‑99 (MQ=255)
       tttAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGtttttttta                                                                                       <  1:2675223/100‑3 (MQ=255)
       tttAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGATAATTGtttttttta                                                                                       >  1:4071871/1‑98 (MQ=255)
         tAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGttttttttaa                                                                                      <  1:3000001/99‑4 (MQ=255)
          aaaaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGttttttttaata                                                                                    >  1:736411/1‑95 (MQ=255)
           aaaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAg                                                                                    >  1:3383835/1‑100 (MQ=255)
           aaaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAg                                                                                    >  2:664002/1‑100 (MQ=255)
           aaaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAg                                                                                    <  1:3679566/100‑1 (MQ=255)
            aaCATCTTTTTGACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCTGTTTGCGGATAACCAAAAATGCAGGTTAATTGttttttttaata                                                                                    <  2:3838133/98‑6 (MQ=255)
             aCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCg                                                                                  >  1:4112777/1‑100 (MQ=255)
                tCTTTTTAACGTCAATAATGACATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAAGAAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGaa                                                                                >  2:1883612/1‑99 (MQ=255)
                     ttAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTc                                                                          <  2:3610673/100‑1 (MQ=255)
                     ttAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAAGGGGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTc                                                                          <  1:1348230/100‑1 (MQ=255)
                       aaaggcAATGATACCAAAGATTAGCTTTATTATCATGTAATCCGTTTAAAAAAAAAAAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTa                                                                        <  2:1658448/95‑1 (MQ=255)
                       aaCGTCAATGATGCCATTGCTTAGCGTTATTATCAGGTAATCTGTTTGCGGAAAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTc                                                                          <  2:3942748/98‑1 (MQ=255)
                       aaCGTCAATGATGCAATTGCTAATCGTCATGAGATGTTAATTTGTTTACGAATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTa                                                                        <  2:1658538/100‑1 (MQ=255)
                         cGTCAATGATGCCATTGCTTAGCGTTACCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCTGGTTAATTGTTTTTTTTAATACCGAAATTTTATatt                                                                      >  2:2717435/1‑100 (MQ=255)
                          gTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTattc                                                                     <  1:1300882/100‑1 (MQ=255)
                          gTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTattc                                                                     >  1:3794292/1‑100 (MQ=255)
                           tCAATGATACCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTatt                                                                      <  1:3663028/98‑1 (MQ=255)
                            cAATGATGCCATTGTTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAACAGTGCAGGTTGATTGTTTTTATTAATAGTGTATTTTTCTatgt                                                                     >  2:3612959/1‑96 (MQ=255)
                               tgctgCGATTGCTTTGTGTTATCATCAGTTATTCCGTTCGCGGTTATCCAAATATGCATGTTAATTTTTTTTTTTAATAGCGAAATGTTCTATTCATTCg                                                                <  2:3778392/97‑1 (MQ=255)
                               tgatgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCg                                                                <  2:3786360/100‑1 (MQ=255)
                               tgatgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCg                                                                <  2:3741025/100‑1 (MQ=255)
                               tgatgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCg                                                                <  1:3421010/100‑1 (MQ=255)
                                  tgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGa                                                             <  1:4009298/100‑1 (MQ=255)
                                  tgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGa                                                             >  1:3099247/1‑100 (MQ=255)
                                    ccATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATc                                                           >  1:3996912/1‑100 (MQ=255)
                                    ccATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATc                                                           <  2:2890946/100‑1 (MQ=255)
                                     cATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATATCTAAATTTTCTATTCATTCGTGATCa                                                          >  2:1742609/1‑100 (MQ=255)
                                     cATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCa                                                          <  2:1509141/100‑1 (MQ=255)
                                        taattAGAGTTATAACTAGGTAACTCGTTTGAAGATAACCAAAACTGCTGGTTAATTGTTTTTTTTAATAGCGAAATTTGCGATTCATTCGTGATCAAg                                                        <  2:3445358/96‑1 (MQ=255)
                                        tGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGa                                                       <  1:2021676/100‑1 (MQ=255)
                                         gCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGAt                                                      >  1:3259337/1‑100 (MQ=255)
                                         gCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGAt                                                      >  1:4049778/1‑100 (MQ=255)
                                         gCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGAt                                                      <  1:1880073/100‑1 (MQ=255)
                                          cTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATc                                                     >  2:2952409/1‑100 (MQ=255)
                                          cTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATc                                                     >  2:4054378/1‑100 (MQ=255)
                                           ttAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCa                                                    <  1:214549/100‑1 (MQ=255)
                                           ttAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCa                                                    <  1:1925268/100‑1 (MQ=255)
                                            tAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCAc                                                   >  1:1286577/1‑100 (MQ=255)
                                            tAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCAc                                                   <  2:3920023/100‑1 (MQ=255)
                                            tAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCAc                                                   >  1:1619424/1‑100 (MQ=255)
                                            tAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATAAc                                                   >  2:2475289/1‑100 (MQ=255)
                                            tAGCGTTATCACCAGGTAATCCGTTTGCGGATAAGCAAAGATGCACGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATCCGTGATCAAGATCAc                                                   <  2:3400309/100‑1 (MQ=255)
                                              gCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGa                                                 <  1:2957424/100‑1 (MQ=255)
                                               cGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGAt                                                <  1:2887955/100‑1 (MQ=255)
                                                ggtATCATCAGGTAATCCGTTTGCGTATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGa                                                 <  1:176707/96‑1 (MQ=255)
                                                gTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCTTGATCACGaat                                               <  1:3593195/100‑3 (MQ=255)
                                                      atcaGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTTTTTTTTTTATTTTCTATTCATTCGTTATCAATATCACGATTCTGt                                           >  2:933322/1‑98 (MQ=255)
                                                      atcaGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTc                                          >  2:3209228/1‑99 (MQ=255)
                                                       tcaGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGc                                        >  1:3651169/1‑100 (MQ=255)
                                                        caGGTAATCCTTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCa                                       <  2:1648757/100‑1 (MQ=255)
                                                         nggTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCaa                                      >  2:531440/2‑100 (MQ=255)
                                                         aGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGACATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCaa                                      >  2:1888876/1‑100 (MQ=255)
                                                         aGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCaa                                      <  1:2008710/100‑1 (MQ=255)
                                                          ggTAATCCGTTTGCGGATAATCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATGCGTGATCAAGATCACGATGCTGTCGCAAt                                     <  2:3889934/100‑1 (MQ=255)
                                                          ggTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTAATTCGTGATCAAGATCACGATGCTGTCGCAAt                                     >  2:875777/1‑100 (MQ=255)
                                                           gTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATg                                    <  1:1107545/100‑1 (MQ=255)
                                                           gTAATCCGTTTGCGGATAAACAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATg                                    <  2:1767758/100‑1 (MQ=255)
                                                             aaTCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTATTATTTAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTa                                  >  2:1804321/1‑100 (MQ=255)
                                                             aaTCCGTTTGCGGATAACCAAAAATGCAGATTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCACTCGTGATCAAGATCACGATGCTGTCGCAATGTa                                  <  2:2488776/100‑1 (MQ=255)
                                                              aTCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTaa                                 >  1:838014/1‑100 (MQ=255)
                                                              aTCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTGTCTATTCATTCGTGATCAAGATCACGATGCTGTCGTAATGTaa                                 >  2:4017096/1‑100 (MQ=255)
                                                               tCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTaaa                                >  1:1720329/1‑100 (MQ=255)
                                                               tCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTaaa                                >  1:1802592/1‑100 (MQ=255)
                                                                 cGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTaaaaa                              >  1:3067922/1‑100 (MQ=255)
                                                                   tttGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTaaaa                               <  2:397671/97‑1 (MQ=255)
                                                                    ttGCGGATAACCAAAAATGCAGG‑‑AATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTg                         <  1:2001551/100‑1 (MQ=255)
                                                                      gCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTg                         <  1:2912212/100‑1 (MQ=255)
                                                                      gCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGc                        <  1:1383209/100‑1 (MQ=255)
                                                                       tccgatctcAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCa                       <  1:1991557/92‑1 (MQ=255)
                                                                       cGGATAACCAAAAATGAAGGATAAATGTTTTTATTAATAGCAAAAATTTCTAATCATTCGTGATCAAGATAACGATGCTGTAGCAATGTAAAAACATAGc                        >  2:2459265/1‑100 (MQ=255)
                                                                        ggATAACCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCa                       >  1:3165861/1‑100 (MQ=255)
                                                                             aCCAAAAATGCAGGTTAATTGTTTTTTTTAATCGCGAAAATATCTATTCATTCGTGATCAACAGCGCGATTGTGTCGCAATGTAAAAACATTGCAAGAAt                  >  2:571607/1‑100 (MQ=255)
                                                                             aCCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAAt                  >  1:2761972/1‑100 (MQ=255)
                                                                             aCCAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAAt                  >  1:2235159/1‑100 (MQ=255)
                                                                              ccAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATc                 <  2:2058945/100‑1 (MQ=255)
                                                                              ccAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATc                 >  1:3840618/1‑100 (MQ=255)
                                                                              ccAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATCCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATc                 >  2:2295417/1‑100 (MQ=255)
                                                                               cAAAAATGCAGGTTAATTGTTTTTTTTATTTGCGAAATTTTCTATTCATTCATGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAAt                  >  2:353332/1‑98 (MQ=255)
                                                                               cAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCa                <  2:2891703/100‑1 (MQ=255)
                                                                               cAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCa                >  1:2295490/1‑100 (MQ=255)
                                                                               cAAAAATGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCa                <  1:2150679/100‑1 (MQ=255)
                                                                               cAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGatcgga               >  2:1991544/1‑95 (MQ=255)
                                                                                aaaaaTGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAg               <  1:1690397/100‑1 (MQ=255)
                                                                                  aaaTGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGt             <  2:451774/100‑1 (MQ=255)
                                                                                     tGCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTaa          >  1:3037092/1‑100 (MQ=255)
                                                                                     tGCAGGTAAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTaa          >  1:2161359/1‑100 (MQ=255)
                                                                                      gCAGGTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTa           >  2:2975728/1‑98 (MQ=255)
                                                                                      gCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTaaaa        >  2:1473203/1‑100 (MQ=255)
                                                                                       cAGGTTAATTGTTTTTTTTAATAGCGAAATGTTCTATTCATTCGTTATTAAGATCACGATACTTTCGCAATATAAAAAAATTGCACGACGTAGGTTaaaa        >  2:2722239/1‑100 (MQ=255)
                                                                                         ggTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATc      >  1:3435946/1‑100 (MQ=255)
                                                                                          gTTAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAAt       <  1:2221839/98‑1 (MQ=255)
                                                                                            tAATTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCAtt   >  2:1184196/1‑100 (MQ=255)
                                                                                             aaTTGTTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCAttt  >  1:652845/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTT  >  NC_000913/1712202‑1712393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: