Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,563,518:1 (A)5→6 100% intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,5131.A94.4% 400.1 / 16.7 124intergenic (+396/‑79)yffP/yffQCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base A (62/54);  total (66/58)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 135 total aligned reads displayed.

TGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG  >  NC_000913/2563417‑2563607
                                                                                                  |                                                                                               
tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAAAGAAAGGGAGTTCCCAACAACAAAATAAAGATGGGAGGGaa                                                                                               >  1:2704925/1‑99 (MQ=255)
   aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaa                                                                                          >  1:3342675/1‑99 (MQ=255)
   aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaa                                                                                          <  2:4078917/100‑2 (MQ=255)
   aTTTTGTGATGCAGATCGATTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAAAAAAAGGAAGTTCCCAACAACAAGATAAAGATGGGAGGTaagaaa                                                                                          >  2:1863587/1‑99 (MQ=255)
     tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  2:113590/100‑4 (MQ=255)
     tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  1:2968985/100‑4 (MQ=255)
     tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  1:1982230/100‑4 (MQ=255)
       tgtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatgaa                                                                                      <  1:1695034/100‑6 (MQ=255)
       tgtgATGCAGATCGCTTTTTCCCATCAGGCATTTTTGTTTAATTAAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatgaa                                                                                      <  2:726328/100‑6 (MQ=255)
        gtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGaaa                                                                                      <  1:3346365/100‑1 (MQ=255)
         ttaTGCAGTTCGCTTTTTCCCTTCAAGCATTTTTAATTAAGTCAATCAACGAAAGGCAGTTCCCAACAACAAATTAAAGATTGGAGGTaaaaaatgagac                                                                                    <  2:2002594/98‑8 (MQ=255)
          gATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACg                                                                                    >  1:3166429/1‑100 (MQ=255)
            tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGt                                                                                  >  1:3871438/1‑100 (MQ=255)
             gCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTg                                                                                 <  1:2308846/100‑1 (MQ=255)
              cAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAATAAAATAAAGATGGGAGGTAAAAAATGAAACGGTgg                                                                                >  2:1391215/1‑100 (MQ=255)
              cAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTgg                                                                                <  2:1168555/100‑1 (MQ=255)
              cAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTgg                                                                                >  1:2578695/1‑100 (MQ=255)
               aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTggg                                                                               >  1:654837/1‑100 (MQ=255)
               aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAACTCAATCAACGAAAGGAAGTTCCCACCAACAAAATAAAGATGGGAGGGAAAAAATGAAACGGTggg                                                                               >  1:1344805/1‑100 (MQ=255)
                gATCGCTTTTTCCTTTTATGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              >  2:142931/1‑100 (MQ=255)
                gATCGCTTTTTCCCTTGAGGCATTTTTGTTTAATGCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              <  2:3897102/100‑1 (MQ=255)
                gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              <  2:3978409/100‑1 (MQ=255)
                gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                              >  1:1472012/1‑100 (MQ=255)
                    gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                          >  1:3897406/1‑100 (MQ=255)
                     cTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGc                                                                        <  2:270619/100‑1 (MQ=255)
                      tttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGc                                                                        <  1:7321/100‑1 (MQ=255)
                          tCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTa                                                                    >  1:1935426/1‑100 (MQ=255)
                            ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  >  1:2343153/1‑100 (MQ=255)
                            ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                  >  1:1329650/1‑100 (MQ=255)
                             cTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTg                                                                 <  1:1801320/100‑1 (MQ=255)
                                cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTgg                                                                >  2:4105196/1‑98 (MQ=255)
                                cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                              >  2:454220/1‑100 (MQ=255)
                                cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                              >  1:384618/1‑100 (MQ=255)
                                 aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGa                                                             >  1:2897166/1‑100 (MQ=255)
                                    cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg                                                          >  1:3993237/1‑100 (MQ=255)
                                    cATTTTTGAGTAATTCAATCATCGAACGGCAGTTCCCAACAACAATATAAAAACAAGAGGTAACAAATCAAACCTTCAGAAGTAGCTTTGCCGGttaacg                                                          >  2:3361527/1‑95 (MQ=255)
                                        tttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAAt                                                      <  1:1460278/100‑1 (MQ=255)
                                           gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt                                                   >  1:879474/1‑100 (MQ=255)
                                            tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg                                                  >  2:2459229/1‑100 (MQ=255)
                                            tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGCGATGGTAATGTTg                                                  <  1:2785775/100‑1 (MQ=255)
                                             ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                                 >  2:880560/1‑100 (MQ=255)
                                             ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                                 <  1:592021/100‑1 (MQ=255)
                                              tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt                                                <  1:4133276/100‑1 (MQ=255)
                                               aaTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGtt                                               <  1:1837264/100‑1 (MQ=255)
                                                 ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg                                             <  1:2984480/100‑1 (MQ=255)
                                                 ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg                                             <  1:840824/100‑1 (MQ=255)
                                                  tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                            <  1:2459243/100‑1 (MQ=255)
                                                  tcaatcaaCGAAAGGAAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                            <  2:3337614/100‑1 (MQ=255)
                                                  tcaagcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                            <  1:2017051/100‑1 (MQ=255)
                                                   caatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCt                                           >  1:1439307/1‑100 (MQ=255)
                                                    aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          >  1:3363241/1‑100 (MQ=255)
                                                    aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          >  2:1685333/1‑100 (MQ=255)
                                                    aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          <  2:3582335/100‑1 (MQ=255)
                                                    aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          <  1:3775992/100‑1 (MQ=255)
                                                    aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                          >  2:588430/1‑100 (MQ=255)
                                                     atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAAAGGAGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt                                         >  2:3838696/1‑100 (MQ=255)
                                                      tcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTa                                        >  2:1768067/1‑100 (MQ=255)
                                                       caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                       >  2:2195035/1‑100 (MQ=255)
                                                       caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                       >  2:2139641/1‑100 (MQ=255)
                                                       caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                       <  1:92686/100‑1 (MQ=255)
                                                       caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                       >  2:635379/1‑100 (MQ=255)
                                                        aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                      >  2:2229790/1‑100 (MQ=255)
                                                         aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTg                                     <  1:3130730/100‑1 (MQ=255)
                                                           gAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa                                    <  1:1232105/99‑1 (MQ=255)
                                                            aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                  >  1:2705764/1‑100 (MQ=255)
                                                                gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                              <  2:1189132/100‑1 (MQ=255)
                                                                 cAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGa                             >  2:276795/1‑100 (MQ=255)
                                                                   gTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAag                           <  1:276798/100‑1 (MQ=255)
                                                                    ttaccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAag                           <  2:2468856/96‑1 (MQ=255)
                                                                    ttCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg                          <  2:1732543/100‑1 (MQ=255)
                                                                      cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                        <  2:2428562/100‑1 (MQ=255)
                                                                      cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                        >  1:2820030/1‑100 (MQ=255)
                                                                      cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGAGGGAAGTGGCTTTACTGGTAATAGAAGAGTTGGATTGCTGTATTGAACTGTGAagg                          >  2:3460751/1‑98 (MQ=255)
                                                                       ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                       <  1:2504773/100‑1 (MQ=255)
                                                                       ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                       >  2:1546311/1‑100 (MQ=255)
                                                                       ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                       >  2:3105122/1‑100 (MQ=255)
                                                                        caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTACTGTTGGGGTGGTGTATTGAAGGGTGCaggtgg                       >  2:3320442/1‑99 (MQ=255)
                                                                        caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga                      >  2:4108043/1‑100 (MQ=255)
                                                                           caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                   >  1:1460174/1‑100 (MQ=255)
                                                                           caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                   <  1:3223046/100‑1 (MQ=255)
                                                                             acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa                 >  2:2835697/1‑100 (MQ=255)
                                                                             acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa                 >  2:1315002/1‑100 (MQ=255)
                                                                              caaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt                <  2:3985119/100‑1 (MQ=255)
                                                                              caaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt                >  1:1053578/1‑100 (MQ=255)
                                                                                aaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa              >  2:3971829/1‑100 (MQ=255)
                                                                                 aaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGaa             <  1:2197845/100‑1 (MQ=255)
                                                                                   tAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc           <  1:738966/100‑1 (MQ=255)
                                                                                    aaaGATGGGGGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc           <  1:541337/99‑1 (MQ=255)
                                                                                     aaGATGGGAGCTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtt         <  2:2705748/100‑1 (MQ=255)
                                                                                      aGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt        >  1:452936/1‑100 (MQ=255)
                                                                                       gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt       >  2:151269/1‑100 (MQ=255)
                                                                                       gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt       <  1:1184211/100‑1 (MQ=255)
                                                                                        aTGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTa      <  2:1988273/100‑1 (MQ=255)
                                                                                        aTGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTaa     <  1:710627/100‑1 (MQ=255)
                                                                                        aTGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTaa     <  1:3943726/100‑1 (MQ=255)
                                                                                         ttggAGGTAAAAAATGAAACGGGGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTAGCTGTATTGAACTGTGAAGGAGCACGCCATGAAACTTTTa      <  2:3055527/97‑1 (MQ=255)
                                                                                         tGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTaa     <  2:2801089/100‑1 (MQ=255)
                                                                                           ggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc   >  2:2804176/1‑100 (MQ=255)
                                                                                            gAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCg  <  2:3661325/100‑1 (MQ=255)
                                                                                            gAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCg  <  2:3878605/100‑1 (MQ=255)
                                                                                                  |                                                                                               
TGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG  >  NC_000913/2563417‑2563607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: