Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,879:1 +G 15.0% intergenic (‑142/+26) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8781.G15.0% 409.0 / 76.2 120intergenic (‑141/+27)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base . (44/58);  new base G (8/10);  total (52/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 128 total aligned reads displayed.

GGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGG  >  NC_000913/2596780‑2596977
                                                                                                   |                                                                                                   
ggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                    >  1:1369555/1‑100 (MQ=255)
 gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                                   <  2:671450/100‑1 (MQ=255)
  ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCa                                                                                                  >  1:4012353/1‑100 (MQ=255)
   tCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCAc                                                                                                 <  2:4029296/100‑1 (MQ=255)
    cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                                <  1:3372287/100‑1 (MQ=255)
     agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATACCGAGAGCGGTTGTCTTGCCGTGCCAGCt                                                                                                      >  2:4008988/1‑94 (MQ=255)
     agagGAAGAGTCCCACAGGAAAGCGGGAGGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                                <  2:312324/99‑1 (MQ=255)
      gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                              >  1:1598223/1‑100 (MQ=255)
      gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                              >  2:4111737/1‑100 (MQ=255)
      gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                              >  1:2450010/1‑100 (MQ=255)
       agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCTAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCa                                                                                             <  1:2910523/100‑1 (MQ=255)
        ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                            <  1:3957111/100‑1 (MQ=255)
        ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                            <  1:3954041/100‑1 (MQ=255)
         gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                           <  2:2818642/100‑1 (MQ=39)
          aaGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAga                                                                                          <  2:3629197/100‑1 (MQ=39)
          aaGACTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAgn                                                                                          <  2:3435717/100‑2 (MQ=38)
             aTTACCACAGTAAAGCGGGATGAAATGGCGGAGTAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAgacaa                                                                                       <  2:3079042/100‑4 (MQ=37)
             aTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                       <  2:1422764/100‑1 (MQ=255)
               tACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGatgatg                                                                                     <  1:1442667/100‑1 (MQ=255)
                aCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAtgatga                                                                                    <  2:900973/100‑1 (MQ=255)
                 ccACAGGAAAGCGGGATGAAATGTCGGGGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGCCTTTCCATGCCAGCTGCACGGCAAGCTGATGaa                                                                                   >  2:839972/1‑100 (MQ=255)
                 ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                                   >  2:2980374/1‑100 (MQ=255)
                 ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                                   <  1:1541919/100‑1 (MQ=255)
                 ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATGCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                                   <  2:3286752/100‑1 (MQ=255)
                   acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                                 <  2:2319215/100‑1 (MQ=255)
                       gAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                                 <  2:3442211/96‑1 (MQ=255)
                       gAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaa                                                                             <  2:982567/100‑1 (MQ=255)
                         aaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                           <  2:2321515/100‑1 (MQ=255)
                          aGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                          <  1:1670894/100‑1 (MQ=255)
                             gtgttGAAACGGCGCAAGAAAGTGCGCCCAATACATATATTCCAACAGCAGAAAACTTTCATAGCCACCTGCACGGCAAGATGATGAACAGAAAAATc                                                                         <  2:3842819/94‑1 (MQ=255)
                             gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATc                                                                         <  2:808416/98‑1 (MQ=255)
                              ggATGAAATGGCGGAGGAATGAGCGTTCATTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACCGCAAGATGTTGAACAGAAAAATCAg                                                                       >  2:2967746/1‑99 (MQ=255)
                              ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                      <  2:1782950/100‑1 (MQ=255)
                              ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                      >  2:609992/1‑100 (MQ=255)
                               gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                     >  2:2123658/1‑100 (MQ=255)
                               gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                     >  2:3845826/1‑100 (MQ=255)
                                    aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCt                                                                >  1:2105899/1‑100 (MQ=255)
                                    aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCt                                                                <  1:1781411/100‑1 (MQ=255)
                                     aTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg                                                               <  2:1369546/100‑1 (MQ=255)
                                      tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGt                                                              >  2:2424265/1‑100 (MQ=255)
                                       ggCGGGGGAATGAGCGTTCAATGCATATATGCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                             <  2:3546406/100‑1 (MQ=255)
                                        gCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                            >  1:3991109/1‑100 (MQ=255)
                                             ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                       >  2:1131190/1‑100 (MQ=255)
                                               aaTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAgg                                                     <  1:2767857/100‑1 (MQ=255)
                                                  gAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggcgg                                                  >  1:4104667/1‑100 (MQ=255)
                                                   aGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAACAATCAGTCCAGCTGTACACCCAggcggc                                                 >  2:405365/1‑100 (MQ=255)
                                                   aGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAGCAGTCCAGCTGTACACCCAggcggc                                                 >  1:3645608/1‑100 (MQ=255)
                                                     cGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                               <  2:3497952/100‑1 (MQ=255)
                                                       ttCAATGCATATATTCCGAGAGCGGGTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                              <  2:2119529/99‑1 (MQ=255)
                                                          aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                          <  1:4009014/100‑1 (MQ=255)
                                                            tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                          >  2:1491075/1‑98 (MQ=255)
                                                            tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                        >  1:4101242/1‑100 (MQ=255)
                                                             gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACATAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                       <  2:2259981/100‑1 (MQ=255)
                                                             gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                       <  2:905289/100‑1 (MQ=255)
                                                              cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                      <  1:3852959/100‑1 (MQ=255)
                                                              cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                      >  1:593488/1‑100 (MQ=255)
                                                               atatatTCCGAGAGCGGTTGTCTTGCCGTGCCCGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCAGCGGGCGACGGCtt                                     >  1:1191729/1‑100 (MQ=255)
                                                               atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCtt                                     <  1:3086132/100‑1 (MQ=255)
                                                               atatacTCCGAGAGCGGTTGTCTTGCAGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCtt                                     <  2:1934799/100‑1 (MQ=255)
                                                                tatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACATCCAGGCGGCGGGCGACGGCTTc                                    <  2:2397303/100‑1 (MQ=255)
                                                                  tatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt                                  <  1:4045802/100‑1 (MQ=255)
                                                                   atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                                 >  1:1480350/1‑100 (MQ=255)
                                                                     tCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAgg                               <  2:2298218/100‑1 (MQ=255)
                                                                      ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                              >  1:185462/1‑100 (MQ=255)
                                                                      ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                              >  1:353403/1‑100 (MQ=255)
                                                                      ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                              <  1:45794/100‑1 (MQ=255)
                                                                       cGAGAGCGGTTGTCTTGCCGTGCCCGATGCACGGCAAGATGATGAACAGAAAACTCAGTCCCGCTGTACACCCACGCGGCGGGCGACCGCTTCATa                                 >  2:1937782/1‑96 (MQ=255)
                                                                       cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt                             <  1:3125359/100‑1 (MQ=255)
                                                                        gagaGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCtt                            >  2:2983361/1‑100 (MQ=255)
                                                                         agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                           <  1:3126502/100‑1 (MQ=255)
                                                                          tgttcatcatCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCg                          >  1:1625306/10‑100 (MQ=25)
                                                                              ggTTGTCTTGCCGTGCCAGCGGCACGGCAAGATGATGAACAGAATAATCAGTCCAGCTGTACACCCCGGCGGCGGGCGACGGCTGCATAGGCTTCGagc                       <  2:1586762/99‑3 (MQ=255)
                                                                               atcatCTTGCCGTGCAGCTGGCACGGCAAGATGATGTACAGAAAAATCAGTCCCGCTGTACACCCAGGCGGCGGGCGACGGCTTCATCGGCTTCGATCa                      >  2:1896401/5‑99 (MQ=25)
                                                                                ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCatg                    <  1:2790746/100‑3 (MQ=255)
                                                                                ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                    <  2:328248/100‑1 (MQ=255)
                                                                                ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                    <  1:1481684/100‑1 (MQ=255)
                                                                                 tGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                   >  1:2258994/1‑100 (MQ=255)
                                                                                 tGTCTTGCCGTGCCAGCTCCACGGCAAGAGGATGAACAGAAAGATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAt                        <  2:679220/95‑1 (MQ=255)
                                                                                  gTCTTGCAGTGCCAGCTGCACGTCTAGATGATGAACAGAAAAAGCACTCCAGCTGTTCACCCAGACTGCGGGCGACGGCTTTTTAGGCTTCGATCAggtc                  <  2:4057725/100‑3 (MQ=255)
                                                                                  atCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGCCGCCCCCATCGGCGTCGATCACGcc                  >  1:3736486/2‑100 (MQ=25)
                                                                                  atCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGcc                  >  1:4065558/2‑100 (MQ=39)
                                                                                      tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAGCg              >  1:1929419/1‑100 (MQ=255)
                                                                                      tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg              >  1:1307454/1‑100 (MQ=255)
                                                                                      tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg              >  1:2969488/1‑100 (MQ=255)
                                                                                      tGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCGGTACACCCAGGCGGCGGGCGACTGTTTCATAGACTTCGATCAGGCCACCg              <  2:1331228/100‑1 (MQ=255)
                                                                                      tGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCAAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCg              >  1:63719/1‑100 (MQ=255)
                                                                                        ccGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTTATAGGCTTCGATCAGGCCACCGAg            <  2:565697/100‑1 (MQ=255)
                                                                                         cGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAgg           <  1:3674851/100‑1 (MQ=255)
                                                                                          gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGc          <  1:3419854/100‑1 (MQ=255)
                                                                                             ccAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctct       >  1:1260836/1‑100 (MQ=255)
                                                                                               aGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCATCCTAGGCTTCGATCAGGCCACCGAGGCGCTgg     >  2:3689139/1‑100 (MQ=255)
                                                                                                 cTGGACGGCAAGATGATGAACAGAAAAAGCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   <  2:3137885/100‑1 (MQ=255)
                                                                                                 cTGCACGGCAAGATGATGAACAGAACAATCAGTCCAGCTGTACACCCAGGCAGCGGGCGACGGCTACATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   >  2:2294045/1‑100 (MQ=255)
                                                                                                 cTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   <  2:3811643/100‑1 (MQ=255)
                                                                                                 cTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   >  1:3412622/1‑100 (MQ=255)
                                                                                                  tGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCGGGCGGCGGGCGACGGCTTCAGAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   <  2:1731904/99‑1 (MQ=255)
                                                                                                  tGCACGGCAAGATGCTGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg   >  2:2020268/1‑99 (MQ=255)
                                                                                                  tGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCgg  >  1:1790143/1‑100 (MQ=255)
                                                                                                  tGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCgg  >  2:2408008/1‑100 (MQ=255)
                                                                                                  tGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCgg  >  1:508399/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGG  >  NC_000913/2596780‑2596977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: