BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,379,768:1	+C	100%	coding (230/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,764	1	.	C	55.8%	312.7 / 300.5	163	coding (234/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (40/32); new base C (50/41); total (90/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

CCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCT  >  NC_000913/3379665‑3379863

 cGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCg                                                                                                        <  2:681382/99‑1 (MQ=255)

    agcaTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑cc                                                                                                  <  1:3623778/100‑1 (MQ=255)

     gcaTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑ccc                                                                                                 >  1:1939632/1‑99 (MQ=255)

      caTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑ccca                                                                                                <  1:1683052/100‑3 (MQ=255)

        tAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑cccaca                                                                                              <  2:3674963/100‑5 (MQ=255)

        tAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑cccaca                                                                                              >  1:284741/1‑96 (MQ=255)

         aaaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑cccacat                                                                                             >  1:714882/1‑95 (MQ=255)

         aaaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACata                                                                                            >  1:474270/1‑100 (MQ=255)

          aaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑cc‑cccaca                                                                                              >  1:1537175/1‑94 (MQ=255)

           aaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACatat                                                                                           <  1:1171906/100‑1 (MQ=255)

             cGGTGAAAGTGCAGGCGCTGTTTCCGCACTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCA‑CC‑CC‑ACAtatata                                                                                        >  2:4114165/1‑100 (MQ=255)

              ggggAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACAtatata                                                                                        <  2:1928234/97‑1 (MQ=255)

              ggTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACAtatata                                                                                        <  2:3604271/100‑1 (MQ=255)

                tGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAg                                                                                      <  2:1152931/100‑1 (MQ=255)

                 gAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGACAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACCCCGCCC‑CC‑ACATATATAAGc                                                                                     >  1:432681/1‑100 (MQ=255)

                 gAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGc                                                                                     >  1:1428626/1‑100 (MQ=255)

                 gAAAGTGCAGGCGCCGTGTACGCTAACGCGGCAGTCTTGGATCAACAACGTCCACCAGCCAGGTTTTCCCCCCTACTACACCG‑CCACc‑acacacatcca                                                                                      >  2:3391727/1‑91 (MQ=255)

                     gTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACg                                                                                 >  1:2655586/1‑100 (MQ=255)

                     gTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGc                                                                                >  1:1818712/1‑100 (MQ=255)

                     gTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGc                                                                                >  1:3990036/1‑100 (MQ=255)

                      tGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGc                                                                                >  2:1904552/1‑100 (MQ=255)

                      tGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGc                                                                                >  1:2661741/1‑100 (MQ=255)

                       gCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCGATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCa                                                                               >  2:2285781/1‑100 (MQ=255)

                       gCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCAc                                                                              >  1:2037064/1‑100 (MQ=255)

                       gCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCAc                                                                              <  1:2806714/100‑1 (MQ=255)

                          ggCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTg                                                                            >  1:3343098/1‑100 (MQ=255)

                            cgcTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGTTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCg                                                                         <  2:2595307/100‑1 (MQ=255)

                              cTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGt                                                                        >  1:2037106/1‑100 (MQ=255)

                               tGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTa                                                                       <  1:2936287/100‑1 (MQ=255)

                               tGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTa                                                                       <  1:909594/100‑1 (MQ=255)

                               tGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTAt                                                                      <  2:581500/100‑1 (MQ=255)

                                gTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATg                                                                     >  1:2745206/1‑100 (MQ=255)

                                   tCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCt                                                                   <  1:587720/100‑1 (MQ=255)

                                     cGCTCTCCCGGCAGGCTTTGATAGTAAAGGTCCATCAGCCGGGTTTCCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtg                                                                <  2:138887/100‑1 (MQ=255)

                                     cGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCttt                                                                 >  2:1790773/1‑100 (MQ=255)

                                      gCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtg                                                                <  1:2271717/100‑1 (MQ=255)

                                      gCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgt                                                               >  1:2612477/1‑100 (MQ=255)

                                      gCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgt                                                               >  1:2459499/1‑100 (MQ=255)

                                       ctctCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgt                                                               <  1:2688870/100‑1 (MQ=255)

                                        tctcCGGGCAGGCTTTGATAGAAAAGGTCCCTCAGCCAGGTTTTCCCGCGTACTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgtg                                                              <  2:614549/100‑1 (MQ=255)

                                        tctcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgtg                                                              <  1:641329/100‑1 (MQ=255)

                                        tctcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTtgtg                                                              <  1:2946459/100‑1 (MQ=255)

                                          tcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTc                                                            <  1:2277066/100‑1 (MQ=255)

                                          tcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTc                                                            >  1:1724178/1‑100 (MQ=255)

                                           cccGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑AGATATATAAGCCACGCACTGGCGTATGCTTTGTGTCt                                                           >  1:3941345/1‑100 (MQ=255)

                                             cGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTc                                                         >  1:1021313/1‑100 (MQ=255)

                                             cGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTc                                                         >  2:3732455/1‑100 (MQ=255)

                                                cAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTcgcg                                                      <  1:1306309/100‑1 (MQ=255)

                                                cAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGt                                                     <  1:1230430/100‑1 (MQ=255)

                                                  ggCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGtc                                                    <  2:2847837/100‑2 (MQ=255)

                                                   gCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGttt                                                   >  2:2760852/1‑100 (MQ=255)

                                                    cTTTGATAGAAAAGGTCCATCAGCCAGTTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTAc                                                 >  2:653852/1‑100 (MQ=255)

                                                    cTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTa                                                  >  2:1047397/1‑100 (MQ=255)

                                                       tGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTAcccc                                              <  1:1215707/100‑1 (MQ=255)

                                                        gctAGAAAGGGTCCATCAGCCAGGTTTTCCCGCGTTATACAGCG‑CA‑CC‑CAATATATAAGCCACGCACTGGAGTATGCACTGTGTCTTCGCGTTAACCGca                                             >  2:31524/3‑100 (MQ=255)

                                                        gATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTAcccc                                              >  2:212766/1‑100 (MQ=255)

                                                        gATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTAcccc                                              >  2:1842639/1‑100 (MQ=255)

                                                          tAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCcaca                                           <  2:1320499/100‑1 (MQ=255)

                                                          tAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCcaca                                           >  1:1077714/1‑100 (MQ=255)

                                                           aGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCcaca                                           <  1:3832394/100‑1 (MQ=255)

                                                           aGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATCTAAGCCACGCCCTGGCGTATGCTTTGTGTCTTCCCGTTTACCCCACAg                                          >  1:812871/1‑100 (MQ=255)

                                                            gAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAg                                          >  1:220534/1‑100 (MQ=255)

                                                             aaaaGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGc                                         <  2:1342207/100‑1 (MQ=255)

                                                               aaGGTCCATCAGCCAGGTTTTCCCGCTTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGc                                         <  2:3614850/98‑1 (MQ=255)

                                                               aaGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTTTTCGCGTTTACCCCACAGCTTa                                      <  2:2400688/100‑1 (MQ=255)

                                                                 ggTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTCGTGTCTTCGCGTTTACCCCACAGCTTAc                                     <  2:3844832/100‑1 (MQ=255)

                                                                 ggTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTAcc                                    <  2:1614301/100‑1 (MQ=255)

                                                                 ggTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTAcc                                    >  2:3845043/1‑100 (MQ=255)

                                                                  gTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTAcc                                    <  1:543167/100‑1 (MQ=255)

                                                                  gTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCg                                   >  1:3083515/1‑100 (MQ=255)

                                                                    ccATCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGa                                  >  1:1610338/1‑100 (MQ=255)

                                                                      aTCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGAcc                                <  1:1108406/100‑1 (MQ=255)

                                                                       tCAGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGAccc                               >  2:2697623/1‑100 (MQ=255)

                                                                         aGCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGC                             >  2:2825978‑M1/1‑100 (MQ=255)

                                                                         aGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCG                            <  1:1336949‑M1/100‑1 (MQ=255)

                                                                          gCCAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCG                            >  1:3599807‑M1/1‑100 (MQ=255)

                                                                           ccAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGC                           <  2:3941319‑M1/100‑1 (MQ=255)

                                                                            cAGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCC                          <  2:2061082‑M1/100‑1 (MQ=255)

                                                                            cAGGTTGTCCCGCGTCCGACAGCGCCC‑CC‑ACCTATATACGCCACGCACTGGCGTATGCGTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCgcgc                           <  2:3518858/99‑1 (MQ=255)

                                                                             aGGTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCA                         <  1:3668168‑M1/100‑1 (MQ=255)

                                                                              ggTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCAt                        >  1:2091760/1‑100 (MQ=255)

                                                                               gtcttCCCGCGTCCTATACCG‑CT‑CC‑TCCTATCTAATCCTCTCACCGTCGTATGCTATGTCCCTTCGCGTTTTCCCCACAGCTTACCTACCCGCGCCAt                        <  2:3201178/95‑1 (MQ=255)

                                                                               ggtttCCCGCGTCCTACACCG‑CC‑CCGACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCTCAGCTTACCGACCCGCGCCAtt                       <  2:224485/98‑1 (MQ=255)

                                                                               gTTTTCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCAtt                       >  2:1665931/1‑100 (MQ=255)

                                                                                 tttCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAg                     <  1:953241/100‑1 (MQ=255)

                                                                                  ttCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGcc                   <  2:3286313/100‑1 (MQ=255)

                                                                                  ttCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGcc                   <  1:1496081/100‑1 (MQ=255)

                                                                                   tCCCGCGTCCTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGcc                   >  1:3998868/1‑100 (MQ=255)

                                                                                     ccGCGTCCTACACCG‑CC‑CC‑ACATATATAATCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTATCCCACAGCTTACCGACCCGCGCCATTAGCCCAc                <  2:2481934/100‑1 (MQ=255)

                                                                                           ccTACACCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTc           >  2:1528519/1‑100 (MQ=255)

                                                                                              acacCGCCC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTg        >  2:555240/1‑100 (MQ=255)

                                                                                              acacCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTgg       >  1:2699348/1‑100 (MQ=255)

                                                                                              acacCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTgg       >  1:3437907/1‑100 (MQ=255)

                                                                                               cacCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTggg      <  1:3151464/100‑1 (MQ=255)

                                                                                                acCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTgggg     <  1:3087579/100‑1 (MQ=255)

                                                                                                 ccG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTggggg    >  2:27900/1‑100 (MQ=255)

                                                                                                 ccG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTggggg    >  2:2234417/1‑100 (MQ=255)

                                                                                                 ccG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTAAGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTggggg    >  2:2485875/1‑100 (MQ=255)

                                                                                                  cG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGc   <  2:3032319/100‑1 (MQ=255)

                                                                                                   g‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCt  <  1:1381777/100‑1 (MQ=255)

CCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCG‑CC‑CC‑ACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCT  >  NC_000913/3379665‑3379863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`