Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,504:1 +T 22.2% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T22.2% 531.6 / 189.5 194intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (89/62);  new base T (19/24);  total (108/86)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 195 total aligned reads displayed.

GATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520407‑4520603
                                                                                                  |                                                                                                   
gATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCt                                                                                                   >  1:241788/1‑100 (MQ=255)
 aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTc                                                                                                  >  1:237915/1‑100 (MQ=255)
  ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTcc                                                                                                 <  2:1782643/100‑1 (MQ=255)
     tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                              <  2:1163583/100‑1 (MQ=255)
      ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCt                                                                                             <  2:387189/100‑1 (MQ=38)
       taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                            <  1:582374/100‑1 (MQ=38)
       taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                            <  2:1173005/100‑1 (MQ=38)
       taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCAATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                            <  1:2608172/100‑1 (MQ=38)
        aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           <  1:3475369/100‑1 (MQ=255)
        aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtta                                                                                           >  2:3596001/1‑99 (MQ=255)
        aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                           <  1:1281326/100‑1 (MQ=38)
        aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                           <  1:617042/100‑1 (MQ=38)
        aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                           <  1:80133/100‑1 (MQ=38)
        aCAGATATTCTATTCTTTTACATGAAATAAACCCGCATACCATTTGAGCGATCGCCCGGTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                            <  2:2950513/99‑1 (MQ=37)
         cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  2:486219/1‑100 (MQ=255)
          aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                         <  2:1036869/100‑1 (MQ=255)
          aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTAc                                                                                         <  2:2279738/100‑1 (MQ=38)
           gagatTTTGTGCTTTTACATGACATATACCGGCATACCATTTGAGCGATCGCCCGCTGTGCCCCGATACTGTAAAGCCGGAGCATGTCTCCGGc                                                                                              <  2:1965794/91‑1 (MQ=21)
           gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                        >  1:3070376/1‑100 (MQ=255)
           gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                        >  1:2068406/1‑100 (MQ=255)
           gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                        <  1:1316230/100‑1 (MQ=38)
            atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAg                                                                                       >  1:3681798/1‑100 (MQ=255)
              atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTa                                                                                     <  2:3917704/100‑1 (MQ=255)
               ttttATTCTTTTACATGAAATAAAACAGCATACTATTTGAGCGTTCGCCCGCTGTTCCCCCATACTGTAAAGACGGAGACCTGCTCCGGCTTTACAGtct                                                                                    >  2:4102048/1‑98 (MQ=255)
                tttATTCTTTTACATGAAATAAACCAGCATACCATTTCACCCATCATCCACTTTTTCCCCATACTGTAAAGGCAGAGCATTGCTTCGGACTTACAGtt                                                                                     >  2:570914/1‑97 (MQ=255)
                 ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTa                                                                                  <  2:851009/100‑1 (MQ=255)
                      cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                             <  2:2010726/100‑1 (MQ=255)
                      cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                             <  1:53759/100‑1 (MQ=255)
                      cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                             >  2:876745/1‑100 (MQ=255)
                       ttttTCTTGAATTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgaga                                                                            >  2:1493209/1‑100 (MQ=255)
                         ttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAt                                                                          <  1:774341/100‑1 (MQ=255)
                           aCATGAAATAAACCATCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATac                                                                        <  1:4073586/100‑1 (MQ=255)
                            cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                       >  1:3602164/1‑100 (MQ=255)
                             aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                      >  2:2382896/1‑100 (MQ=255)
                              tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:816802/1‑100 (MQ=255)
                               gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                    <  2:130789/100‑1 (MQ=255)
                               gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                    >  1:1084678/1‑100 (MQ=255)
                               gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                    >  1:2894053/1‑100 (MQ=255)
                                aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacaagat                                                                   >  1:3548979/1‑96 (MQ=255)
                                aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                   >  1:1653840/1‑100 (MQ=255)
                                       ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                            >  2:1927178/1‑100 (MQ=255)
                                         aGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                          >  2:294268/1‑100 (MQ=255)
                                          gCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATaa                                                         >  1:3451865/1‑100 (MQ=255)
                                            aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       >  2:3128800/1‑100 (MQ=255)
                                             tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                      >  2:2236065/1‑100 (MQ=255)
                                             tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                      >  1:226353/1‑100 (MQ=255)
                                              aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAGGCCGGGGACTTGCTCCGGTTTTACAGTATTACTGGGAATACACCCATCAAGCTTAATCGa                                                     >  2:2094063/1‑100 (MQ=255)
                                                cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                   >  1:2619416/1‑100 (MQ=255)
                                                     gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                              <  1:3873266/100‑1 (MQ=255)
                                                        cGACCGCCCGCTCTTCCCCCATCCTGTAAAGCCGGAGACATGCCCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAata                                           <  2:3070875/100‑1 (MQ=255)
                                                         gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAata                                           >  1:3767349/1‑99 (MQ=255)
                                                            cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       >  2:699303/1‑100 (MQ=255)
                                                             gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  1:3286796/1‑100 (MQ=255)
                                                             gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      <  1:905604/100‑1 (MQ=255)
                                                             gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  1:3624945/1‑100 (MQ=255)
                                                               ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcccc                                    <  1:3644326/100‑1 (MQ=255)
                                                                 gCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtt                                  >  1:482049/1‑100 (MQ=255)
                                                                  cTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttt                                 >  2:1703818/1‑100 (MQ=255)
                                                                   tGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGTTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                >  2:2200678/1‑100 (MQ=255)
                                                                    gTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               >  1:1874881/1‑100 (MQ=255)
                                                                      tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                             <  1:1072900/100‑1 (MQ=255)
                                                                       cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  2:3691487/1‑100 (MQ=255)
                                                                       cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  2:265337/1‑100 (MQ=255)
                                                                       cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  2:2484790/1‑100 (MQ=255)
                                                                       cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:871936/1‑100 (MQ=255)
                                                                       cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:624041/1‑100 (MQ=255)
                                                                       cagtaaTACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:3144019/6‑100 (MQ=25)
                                                                        ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  2:3482886/100‑1 (MQ=255)
                                                                        ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           >  1:2493972/1‑100 (MQ=255)
                                                                         cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          >  2:322445/1‑100 (MQ=255)
                                                                         cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          <  1:4062353/100‑1 (MQ=255)
                                                                          ccgtACTGTAAAGCCGGAGACATGCTCCGGCTCTACAGTATTACTGAGAATACACCCCTCAACCCTAACCGAAGGTTCACTATCGCACCTTTTGGATCg                          >  2:751280/4‑99 (MQ=255)
                                                                             tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                      <  1:2689185/100‑1 (MQ=255)
                                                                              aCTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                     >  1:3491361/1‑100 (MQ=38)
                                                                               cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGCGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCTTTTTGATCACCAg                      >  2:1437373/1‑98 (MQ=25)
                                                                               cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  1:1889059/1‑100 (MQ=38)
                                                                               cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  2:2729168/1‑100 (MQ=38)
                                                                                 gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCCTCAAGCATACTCGAAGCTTCAATATCGCCCCTTTTTGATCGCCAGCGa                   >  2:1271870/1‑99 (MQ=255)
                                                                                 gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:2197870/1‑100 (MQ=255)
                                                                                   aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                <  2:2083229/100‑1 (MQ=255)
                                                                                    naGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCAATTCg               >  2:3355785/2‑100 (MQ=255)
                                                                                    acgCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  1:2795303/3‑100 (MQ=38)
                                                                                    aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               <  1:1179769/100‑1 (MQ=255)
                                                                                    aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:2590862/1‑100 (MQ=255)
                                                                                    aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  1:538306/1‑100 (MQ=255)
                                                                                    aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  1:282041/1‑100 (MQ=255)
                                                                                    aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTGAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:2902441/1‑100 (MQ=255)
                                                                                    aaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:1348548/1‑100 (MQ=38)
                                                                                     aGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt              >  2:1252697/1‑100 (MQ=38)
                                                                                      gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc             >  1:3028452/1‑100 (MQ=255)
                                                                                       ccGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa            >  1:2582607/1‑100 (MQ=255)
                                                                                       ccGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa            >  1:655113/1‑100 (MQ=255)
                                                                                        cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCTAGCATACTCGAAGGTTCAATATCGGCCCTTTTTGATCGCCAGCGATTCGTCAc           <  2:3885125/100‑1 (MQ=255)
                                                                                         ggAGACATGCTCCGGCTTTACAGTATTACTCAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCCTTTTGATCGCCAGCGATTCGTCa            <  2:2120448/98‑1 (MQ=255)
                                                                                           agaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGt        <  1:765092/100‑1 (MQ=255)
                                                                                              cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     <  2:2845806/100‑1 (MQ=255)
                                                                                              cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  2:1100712/1‑100 (MQ=255)
                                                                                               aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  2:3381464/1‑99 (MQ=255)
                                                                                               aTGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg    <  1:1486975/100‑1 (MQ=255)
                                                                                                 gCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  >  1:1022467/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520407‑4520603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: