Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 497,139:1 (T)5→6 100% intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913497,1341.T94.8% 382.7 / 12.7 116intergenic (+140/‑41)htpG/adkprotein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base T (47/62);  total (49/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228
                                                                                                     |                                                                                                
tttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc                                                                                                   >  1:3842337/1‑100 (MQ=255)
  tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtt                                                                                                >  1:2377953/1‑100 (MQ=255)
  tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtt                                                                                                >  1:2296817/1‑100 (MQ=255)
  gcagcatcTGCACTCTCCGCAATTTATCTCGCCATGAACCGATTCTGCCCCATGTGCCTTTCTTGAGGCAAGCCCCGGTTGGTGTTATCTTTTATCGc                                                                                                   >  2:1855392/5‑98 (MQ=255)
    atcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttt                                                                                             <  1:3223088/100‑1 (MQ=255)
    atcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttt                                                                                             >  1:1661836/1‑100 (MQ=255)
     tcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttt                                                                                            <  1:397422/100‑1 (MQ=255)
      catcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTACCGTTTATCGCtttttt                                                                                           >  2:2412910/1‑99 (MQ=255)
       atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                          >  1:3968192/1‑98 (MQ=255)
       atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                          <  2:2745080/100‑3 (MQ=255)
        tcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttttt                                                                                           >  2:1285279/1‑97 (MQ=255)
         cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaa                                                                                        >  1:4087623/1‑96 (MQ=255)
          tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                       <  1:1692842/100‑6 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAATCGTTTCAGTCCTAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  2:3315572/100‑1 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  1:1409760/100‑1 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   >  2:2864927/1‑100 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  2:639247/100‑1 (MQ=255)
                ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTc                                                                                 >  1:2048268/1‑100 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                >  1:140575/1‑100 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:1089457/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:1507593/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGCTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:301079/100‑1 (MQ=255)
                  ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGTTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               >  1:28882/1‑100 (MQ=255)
                  ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               <  1:3522516/100‑1 (MQ=255)
                  ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               >  1:1901372/1‑100 (MQ=255)
                  ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               >  2:3990144/1‑100 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  1:607327/100‑1 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  1:53817/1‑100 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  1:3599131/100‑1 (MQ=255)
                       aaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAt                                                                          <  2:1495303/100‑1 (MQ=255)
                       aaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAt                                                                          >  1:3011164/1‑100 (MQ=255)
                        aTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAtt                                                                         <  1:2140009/100‑1 (MQ=255)
                          tATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAtttt                                                                       <  1:304432/100‑1 (MQ=255)
                              tcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgg                                                                   <  1:2001329/100‑1 (MQ=255)
                               cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATAAAAAAAAAAAAAAAATTCGACACATTTTAAgg                                                                   <  2:2028118/99‑1 (MQ=255)
                               cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  >  2:2769509/1‑100 (MQ=255)
                               cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  <  1:381114/100‑1 (MQ=255)
                                  cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAt                                                               >  2:2726217/1‑100 (MQ=255)
                                  cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAt                                                               >  2:816551/1‑100 (MQ=255)
                                   aTTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtt                                                              >  1:435704/1‑100 (MQ=255)
                                    ttAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAttt                                                             >  2:65308/1‑100 (MQ=255)
                                    ttAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAttt                                                             >  2:1090470/1‑100 (MQ=255)
                                     tAACCGTTTCAGCCCCAGGAGCCTTTCTAGAGGCAATCGCCAGCTGGTGGCATCGTTTATCGCTTTTTCCAGAAAATTCGCCAAATTTTAAGGAGAtttt                                                            >  2:2234016/1‑100 (MQ=255)
                                      aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           >  1:2854781/1‑100 (MQ=255)
                                       aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGGTTTTCg                                                          >  1:2837801/1‑100 (MQ=255)
                                       aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCg                                                          >  1:4135775/1‑100 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  1:515868/100‑1 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         >  2:3256044/1‑100 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGACTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  2:1697544/100‑1 (MQ=255)
                                          gTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGaaa                                                       >  2:746160/1‑100 (MQ=255)
                                          gTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCaa                                                       <  2:556401/100‑1 (MQ=255)
                                               aGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGt                                                  <  2:4125400/100‑1 (MQ=255)
                                                gCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa                                                 <  1:2391012/100‑1 (MQ=255)
                                                gCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa                                                 >  2:164634/1‑100 (MQ=255)
                                                  cccAGGTGCCTTTCTTGAGGCAATCGCCTGGTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               >  1:3306845/1‑100 (MQ=255)
                                                       gTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCt                                          >  2:1133739/1‑100 (MQ=255)
                                                         gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGc                                        >  1:724841/1‑100 (MQ=255)
                                                            tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTg                                     <  1:1684669/100‑1 (MQ=255)
                                                             ttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTgg                                    <  2:3289430/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAATTTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:3653941/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   >  1:671074/1‑100 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTACCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:2176686/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGCTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:2086407/100‑1 (MQ=255)
                                                               cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg                                  <  1:1905807/100‑1 (MQ=255)
                                                               cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg                                  >  1:3588884/1‑100 (MQ=255)
                                                                ttGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATGTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcgc                                 >  2:2316867/1‑100 (MQ=255)
                                                                 tgcggtaaTCGCCTGTTGGTGGTATAATGTATTGCTTGTTTCAACAAATTCGACACATTTTAGGGGGATTCTCGCTATGCGTATCATTCTGCTTGgcgc                                 <  2:3113868/93‑1 (MQ=255)
                                                                   aGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTcc                              <  1:1547454/100‑1 (MQ=255)
                                                                   aGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTcc                              <  1:2333617/100‑1 (MQ=255)
                                                                    ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             >  1:1330922/1‑100 (MQ=255)
                                                                    ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             >  1:1052683/1‑100 (MQ=255)
                                                                    ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             <  1:3316485/100‑1 (MQ=255)
                                                                    ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             >  1:3905491/1‑100 (MQ=255)
                                                                      cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           <  1:545914/100‑1 (MQ=255)
                                                                      cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           >  2:2984386/1‑100 (MQ=255)
                                                                        aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg                         >  2:3744510/1‑100 (MQ=255)
                                                                         tCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcgc                        >  1:3144047/1‑100 (MQ=255)
                                                                         tCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcgc                        <  1:683193/100‑1 (MQ=255)
                                                                           gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  2:14543/100‑1 (MQ=255)
                                                                           gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:938814/100‑1 (MQ=255)
                                                                            ccTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCggg                     <  1:3657966/100‑1 (MQ=255)
                                                                             cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    <  1:1002271/100‑1 (MQ=255)
                                                                             cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    >  1:2576273/1‑100 (MQ=255)
                                                                             cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    >  1:1544060/1‑100 (MQ=255)
                                                                              tGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg                         <  2:1705616/94‑1 (MQ=255)
                                                                              tGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGa                   >  2:1771029/1‑100 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCTACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  2:1526877/100‑1 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  1:994530/100‑1 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               >  2:2445565/1‑100 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  2:2360138/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:2681416/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  2:424388/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:2445579/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:3227229/100‑1 (MQ=255)
                                                                                     ggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACTTTTTAAGTGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            <  1:937274/100‑1 (MQ=255)
                                                                                     ggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            >  1:2281414/1‑100 (MQ=255)
                                                                                      gtATCGTTTATCGCTTTTTTCAAAAAATTCTACACGTTTTAAGGGGGGTTTCGCAATGCGTATCATTCTGCTTGGCGCGCCGGGCGCGGGGCAAGGGAc            <  2:3579185/99‑1 (MQ=255)
                                                                                       tATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTc          <  1:112633/100‑1 (MQ=255)
                                                                                           gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGc      <  1:2725540/100‑1 (MQ=255)
                                                                                           gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGc      <  2:75257/100‑1 (MQ=255)
                                                                                                     |                                                                                                
TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: