New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4632232157 (1.060)9 (0.060) 6/170 NT 5.6% coding (1500/1938 nt) slt lytic murein transglycosylase, soluble
?NC_000913 = 4632249 156 (1.110)coding (1517/1938 nt) slt lytic murein transglycosylase, soluble
Rejected: Frequency below cutoff threshold.

AAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4632131‑4632232
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGCTCT  <  NC_000913/4632249‑4632151
                                                                                                                                                                                                         
AAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGG                                                                                                       <  1:2672550/100‑1
 AGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGC                                                                                                      <  2:208193/100‑1
 AGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGC                                                                                                      <  2:1945313/100‑1
   CTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACATCCTTCTTTTCAAACGCTACACCAGCGGTAAGAAAATCCCGCAAAGCTATGCGATG                                                                                                        >  2:199168/1‑96
   CTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAGACGCTACACCAGCGGTAAGGAGATCCCGCAACGCCATGCGATGGCGC                                                                                                    >  2:784279/1‑100
                     GAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCG                                                                                  <  2:1085226/100‑1
                         GATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCAT                                                                              <  1:1605139/100‑1
                         GATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCAT                                                                              <  2:2594197/100‑1
                         GATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCAT                                                                              <  2:3096583/100‑1
                          ATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCATC                                                                             <  1:293512/100‑1
                          ATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCATC                                                                             <  2:2935050/100‑1
                           TTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCATCG                                                                            <  2:1461762/100‑1
                                GCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGCTCTCCTGACGAGCAATCGCCATCGCATAG                                                                       <  2:212589/100‑1
                                                                      GTAAGGAGATCCCGCAAAGCTACGCGATGGCGCTCTCCTGACGAGTAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAA                                 <  1:784285/100‑1
                                                                                                  GGCGCTCTCCTGACGAGCAATCGCGATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGC     <  1:2331913/100‑1
                                                                                                  GGCGCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGC     <  1:3697978/100‑1
                                                                                                  GGCGCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGC     <  1:767705/100‑1
                                                                                                    CGCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGCTC   <  1:2818662/100‑1
                                                                                                     GCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGCTCT  <  1:3837989/100‑1
                                                                                                     GCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGCTCT  <  1:2504693/100‑1
                                                                                                                                                                                                         
AAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4632131‑4632232
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgCTCTCCTGACGAGCAATCGCCATCGCATAGCTTTGCGGGATCTCCTTACCGCTGGTGTAGCGTTTGAAAAGATCGTTGTAAGCCAGCGGGAATCGCTCT  <  NC_000913/4632249‑4632151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.