BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	216,568	C→T	100%	A146T (GCC→ACC)	yaeF ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,568	0	C	T	99.4%	636.8 / ‑4.7	190	A146T (GCC→ACC)	yaeF	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (82/106); total (84/106)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

ACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAA  >  NC_000913/216475‑216662

  ggATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCgg                                                                                        >  1:728427/1‑100 (MQ=255)

   gATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGt                                                                                       <  2:2576655/100‑1 (MQ=255)

   gATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGt                                                                                       <  1:3588619/100‑1 (MQ=255)

       cAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGata                                                                                   <  2:3543236/100‑1 (MQ=255)

       cAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGata                                                                                   >  2:2919797/1‑100 (MQ=255)

       cAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGata                                                                                   >  1:3882326/1‑100 (MQ=255)

       cAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGat                                                                                    >  2:3607025/1‑99 (MQ=255)

         gTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATc                                                                                 >  2:1895734/1‑100 (MQ=255)

           gAGCCCATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCAGCGATAGTTAGAAACGCTATATTAGATTTTATTGGTAAACGCGGTGa                                                                                     >  2:2902209/1‑94 (MQ=255)

           gAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtg                                                                               >  1:2852281/1‑100 (MQ=255)

            aGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtgt                                                                              <  2:939527/100‑1 (MQ=255)

             gCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtgtg                                                                             >  1:2022788/1‑100 (MQ=255)

             gCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtgtg                                                                             <  1:3722579/100‑1 (MQ=255)

               acaTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGc                                                                           <  1:2145939/100‑1 (MQ=255)

               acaTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGc                                                                           <  1:3499717/100‑1 (MQ=255)

                caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTgg                                                                            <  1:1062607/98‑1 (MQ=255)

                caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCt                                                                          <  2:593334/100‑1 (MQ=255)

                caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCt                                                                          >  1:1127877/1‑100 (MQ=255)

                caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCt                                                                          >  2:3764925/1‑100 (MQ=255)

                  tCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGCTATCTGTGGCtag                                                                        <  1:2807887/100‑3 (MQ=255)

                  tCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttg                                                                        <  1:2082528/100‑1 (MQ=255)

                   cTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgt                                                                       <  1:1003773/100‑1 (MQ=255)

                    tGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgtt                                                                      >  1:4047172/1‑100 (MQ=255)

                     ggCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgttg                                                                     >  2:2084504/1‑100 (MQ=255)

                     ggCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgttg                                                                     <  1:1494674/100‑1 (MQ=255)

                        gAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgg                                                                  <  2:3109567/100‑1 (MQ=255)

                         aGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCggg                                                                 <  1:1650985/100‑1 (MQ=255)

                         aGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCggg                                                                 >  1:3050014/1‑100 (MQ=255)

                         aGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCggg                                                                 <  2:868488/100‑1 (MQ=255)

                          gTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                <  2:3039788/100‑1 (MQ=255)

                          gTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                >  1:395559/1‑100 (MQ=255)

                          gTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                >  1:77541/1‑100 (MQ=255)

                          gTCACAATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCggg                                                                 <  2:3847652/99‑1 (MQ=255)

                           tCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                               >  1:3674291/1‑100 (MQ=255)

                            cACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTTCGGGGTa                                                              <  1:2174501/100‑1 (MQ=255)

                            cACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGTGGGGTa                                                              <  1:2000733/100‑1 (MQ=255)

                             aCCATAAAGGGAATAAATTCGACATTGCCGCGACAGTTGTAACCACTATCTTTGACTTTATTAGTAAACGCGACCATATCTGTGGCttgtt                                                                      >  2:1018006/1‑91 (MQ=255)

                             aCCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                                                              <  1:3337577/99‑1 (MQ=255)

                             aCCATAAAGGGAATAAATTCGACAATGCCGCGATAGTGATAACCTCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTaa                                                             <  2:244940/100‑1 (MQ=255)

                              ccATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAg                                                            >  2:3850022/1‑100 (MQ=255)

                               cATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGa                                                           >  1:1948427/1‑100 (MQ=255)

                               cATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGAGATATCTGTGGCTTGTTGCGGGGTAAGa                                                           >  2:1138891/1‑100 (MQ=255)

                               cATAAAGGGAATAAATTCGACAATGCCGCGATAGGTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAg                                                            <  2:1725648/99‑1 (MQ=255)

                                    aGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCgg                                                      >  1:3820987/1‑100 (MQ=255)

                                    aGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGACCCgg                                                      <  2:400474/100‑1 (MQ=255)

                                     gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                     >  1:993646/1‑100 (MQ=255)

                                      ggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGa                                                    >  1:1763001/1‑100 (MQ=255)

                                       gAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGAc                                                   <  1:1583060/100‑1 (MQ=255)

                                        aaTAACGTCGGCGATGGCTCGATAGTTATAACCGGTCTGCTTTCTTTTATGGGTTAACGTAGTGATATCAGGTGCTTGTTGCGGGGTAAGATCCggg                                                     <  2:2045752/97‑1 (MQ=255)

                                        aaTAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGAc                                                   >  2:3744132/1‑99 (MQ=255)

                                        aaTAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACt                                                  <  2:1771117/100‑1 (MQ=255)

                                            aaTTCGACAATGCCGCGATCGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATATGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                              >  2:3946661/1‑100 (MQ=255)

                                            aaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                              <  2:3203369/100‑1 (MQ=255)

                                             aTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTaa                                             <  1:3007619/100‑1 (MQ=255)

                                              ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                            <  2:4011494/100‑1 (MQ=255)

                                              ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                            <  2:3263237/100‑1 (MQ=255)

                                              ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                            <  1:2414134/100‑1 (MQ=255)

                                              ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                            >  2:2017551/1‑100 (MQ=255)

                                               tCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGa                                           >  1:574530/1‑100 (MQ=255)

                                                cGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGAc                                          >  2:507455/1‑100 (MQ=255)

                                                cGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGAc                                          <  1:3530309/100‑1 (MQ=255)

                                                cGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGAc                                          <  2:3584675/100‑1 (MQ=255)

                                                 gACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACg                                         <  1:1926815/100‑1 (MQ=255)

                                                  aCAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTCGCGGGGTAAGAGCCGGGACTCGTAAGAc                                          <  2:2496898/98‑1 (MQ=255)

                                                   cAATGCCGCGATAGTTATACCCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACgca                                       >  2:842300/1‑98 (MQ=255)

                                                   cAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGaa                                       >  2:2950777/1‑100 (MQ=255)

                                                       gCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGc                                   <  1:2508626/100‑1 (MQ=255)

                                                       gCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGc                                   >  1:950653/1‑100 (MQ=255)

                                                        ccGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCt                                  <  1:3997945/100‑1 (MQ=255)

                                                         cgcgATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCtt                                 <  2:658324/100‑1 (MQ=255)

                                                         cgcgATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCtt                                 >  2:1681462/1‑100 (MQ=255)

                                                          gcgATAGTGATAACCTCTATCTTTGATTTTATTGGTAAACGCGGTTATATCTGTCGCTTTTTGCGGTGTAAGATCCGCGACTCGTAAGACGAAAAGCTTa                                <  2:801389/100‑1 (MQ=255)

                                                            gATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATc                              <  2:3381151/100‑1 (MQ=255)

                                                             aTAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTCAGATCCGGGACTCGTAAGACGAAAAGCTTATCa                             <  2:343375/100‑1 (MQ=255)

                                                              tAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCAc                            <  1:526246/100‑1 (MQ=255)

                                                                gTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTa                          >  1:2066873/1‑100 (MQ=255)

                                                                  tataACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATg                        <  2:2796486/100‑1 (MQ=255)

                                                                    taACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCt                      >  2:2746462/1‑100 (MQ=255)

                                                                    taACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCt                      >  1:2480500/1‑100 (MQ=255)

                                                                      aCCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTc                    <  1:2239163/100‑1 (MQ=255)

                                                                              cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGGAAAGCTTATCACTATGCTTCATCGCttt            >  2:2688225/1‑100 (MQ=255)

                                                                              cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttt            >  2:3149309/1‑100 (MQ=255)

                                                                              cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCAGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtt             >  2:905972/1‑99 (MQ=255)

                                                                               tttGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttt           >  1:2351253/1‑100 (MQ=255)

                                                                               tttGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACCAAAAGCTTATCACTATGCTTCATCGCtttt           <  1:2608356/100‑1 (MQ=255)

                                                                                ttGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttttt          <  1:3238955/100‑1 (MQ=255)

                                                                                  gATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTa        <  2:3285343/100‑1 (MQ=255)

                                                                                  gATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTa        >  2:2314536/1‑100 (MQ=255)

                                                                                    ttttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTa        <  2:2151711/98‑1 (MQ=255)

                                                                                    ttttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAg      >  2:1566031/1‑100 (MQ=255)

                                                                                     tttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg     <  2:4106363/100‑1 (MQ=255)

                                                                                     tttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg     <  2:470017/100‑1 (MQ=255)

                                                                                     tttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg     <  1:894449/100‑1 (MQ=255)

                                                                                     tttATTGGTAAACGCGGTGATATCTGTGGCTTGGTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCACCGCTTTTTTAAgg     <  2:3445098/100‑1 (MQ=255)

                                                                                      ttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTGCATCGCTTTTTTacg      >  1:3710804/1‑96 (MQ=255)

                                                                                       tATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGa   <  2:1991315/100‑1 (MQ=255)

                                                                                        aTTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGTAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGaa  >  2:2241424/1‑100 (MQ=255)

                                                                                        aTTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGaa  >  2:361274/1‑100 (MQ=255)

                                                                                        aTTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGa   <  2:54662/99‑1 (MQ=255)

                                                                                         ttGGTAAACGCGGTGATATCTGAGGCTTGTTGCGGGGTAAGATCGGGGCCTCGTAAGACGACACGCTTATACCTATGCTTCATCGCTTTTATCAggg    >  2:1365739/1‑97 (MQ=255)

ACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAA  >  NC_000913/216475‑216662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`