Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,462,065 T→G 60.7% G391G (GGT→GGG paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,462,0650TG60.7% 97.5 / 110.8 104G391G (GGT→GGGpaaJ3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (13/28);  new base G (30/33);  total (43/61)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGT  >  NC_000913/1461969‑1462164
                                                                                                |                                                                                                   
gggAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAg                                                                                                  >  1:896911/1‑100 (MQ=255)
 ggAATGCGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAgg                                                                                                 <  2:3327716/100‑1 (MQ=255)
 ggAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAgg                                                                                                 >  1:4008131/1‑100 (MQ=255)
 ggAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAgg                                                                                                 <  2:617903/100‑1 (MQ=255)
  gAATGAGTGGTGCCCGCCTGGCACTGTCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                >  1:2508237/1‑100 (MQ=255)
  aaaTGAGTGGTGCCCGCCTGGCCCTGGCTGCCAGCAATGAGCTGCACCGGCGCAACGGCCGTTACGCACTGCGAACCATGCGCGTCGCGGCCGGTc                                                                                                    >  2:3730195/2‑96 (MQ=255)
   aaTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGc                                                                                               >  2:1189856/1‑100 (MQ=255)
   aaTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGc                                                                                               >  1:3283297/1‑100 (MQ=255)
      gAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATc                                                                                            <  1:1456266/100‑1 (MQ=255)
       aGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCgggcagggcacca                                                                                           >  1:4083100/1‑97 (MQ=255)
       aGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCAGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGACATCg                                                                                           >  1:1002802/1‑100 (MQ=255)
        gtggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGc                                                                                          >  2:1540688/1‑100 (MQ=255)
           ttccgatcttGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCAt                                                                                       <  2:915062/91‑1 (MQ=255)
            tgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATg                                                                                      <  1:1226530/100‑1 (MQ=255)
              cccGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGAt                                                                                    <  1:1170416/100‑1 (MQ=255)
               ccGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGAtt                                                                                   >  1:3182349/1‑100 (MQ=255)
                  ccTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTg                                                                                <  1:287783/100‑1 (MQ=255)
                  ccTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTg                                                                                <  2:1310852/100‑1 (MQ=255)
                    tGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCAtagatcggaa                                                                              >  1:915068/1‑91 (MQ=255)
                     ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                             <  2:1571868/100‑1 (MQ=255)
                     ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                             <  1:842903/100‑1 (MQ=255)
                     ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                             <  2:3436891/100‑1 (MQ=255)
                     ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                             <  2:3839886/100‑1 (MQ=255)
                     ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAg                                                                             <  2:2452261/100‑1 (MQ=255)
                         cTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtg                                                                         >  1:1872205/1‑100 (MQ=255)
                         cTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCgtg                                                                         >  1:3231304/1‑100 (MQ=255)
                         cTGGCTGCCAGCCATGAGCTGCATCGGCCTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTCGAGCgtg                                                                         >  2:1337612/1‑100 (MQ=255)
                          tGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                        <  2:3982015/100‑1 (MQ=255)
                           ggCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGtt                                                                       <  2:2430716/100‑1 (MQ=255)
                           ggCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGtt                                                                       <  2:381413/100‑1 (MQ=255)
                           ggCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGtt                                                                       >  2:1669921/1‑100 (MQ=255)
                             cTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTg                                                                     <  1:1042050/100‑1 (MQ=255)
                             cTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTg                                                                     <  1:1042183/100‑1 (MQ=255)
                              tGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGa                                                                    <  1:682211/100‑1 (MQ=255)
                               gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAg                                                                   <  2:2222748/100‑1 (MQ=255)
                                ccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                  <  2:4035028/100‑1 (MQ=255)
                                 cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCa                                                                 <  2:2183343/100‑1 (MQ=255)
                                 cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCa                                                                 <  1:2927776/100‑1 (MQ=255)
                                  agccaTGAGCTGCATCGGCGTAACTGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCat                                                                <  1:1064963/100‑1 (MQ=255)
                                     caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCatat                                                              >  1:99041/1‑99 (MQ=255)
                                     caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATc                                                             <  2:3231284/100‑1 (MQ=255)
                                     caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATc                                                             <  1:553365/100‑1 (MQ=255)
                                     caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATc                                                             <  2:734186/100‑1 (MQ=255)
                                       tGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCaa                                                           <  1:1332835/100‑1 (MQ=255)
                                       tGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCaa                                                           >  1:3794430/1‑100 (MQ=255)
                                       tGAGCTGATGCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCatat                                                              <  2:1383735/97‑1 (MQ=255)
                                        gAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAc                                                          <  2:1309552/100‑1 (MQ=255)
                                          gCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCt                                                        >  2:2849619/1‑100 (MQ=255)
                                          gCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCt                                                        >  1:2247007/1‑100 (MQ=255)
                                           cTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTg                                                       >  2:2838818/1‑100 (MQ=255)
                                            tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                      <  2:379276/100‑1 (MQ=255)
                                            tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                      <  2:3674113/100‑1 (MQ=255)
                                             gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCg                                                     >  2:3027112/1‑100 (MQ=255)
                                             gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCg                                                     <  1:1703682/100‑1 (MQ=255)
                                             gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCg                                                     <  2:1454598/100‑1 (MQ=255)
                                             gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCg                                                     <  1:2249159/100‑1 (MQ=255)
                                             gCATCGGCGTAACGGTCGTTACGCAGTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCg                                                     <  1:2858957/100‑1 (MQ=255)
                                               aTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                   <  1:2063137/100‑1 (MQ=255)
                                               aTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                   <  2:1184653/100‑1 (MQ=255)
                                               aTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                   <  2:1646287/100‑1 (MQ=255)
                                                 cGGCGTAGCGGTCGTTACGCATTGTGCACGATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                                 <  2:72721/100‑1 (MQ=255)
                                                 cGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                                 <  2:3883818/100‑1 (MQ=255)
                                                 cGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGGCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTa                                                 <  1:3713016/100‑1 (MQ=255)
                                                  ggCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAc                                                >  1:1530400/1‑100 (MQ=255)
                                                    cGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAccc                                              <  2:1262256/100‑1 (MQ=255)
                                                    cGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAccc                                              >  2:2065678/1‑100 (MQ=255)
                                                     gTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCt                                             <  1:1528176/100‑1 (MQ=255)
                                                              gTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATa                                    >  1:3732992/1‑100 (MQ=255)
                                                              gTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATa                                    >  1:2923887/1‑100 (MQ=255)
                                                              gTTACGCATGGTGCACTATGTGCATCGGTGTCGGTCAGGGCCTCGCCAGTCTTCTGCCCCGTGACTGAGCATACCCACCTGCGCGCACCCTGCAATGAt                                     >  2:2574318/1‑99 (MQ=255)
                                                               ttACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                   >  1:2537421/1‑100 (MQ=255)
                                                                 aCGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAAcc                                 <  1:2833825/100‑1 (MQ=255)
                                                                  cGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCa                                >  1:2628982/1‑100 (MQ=255)
                                                                   ggattgatcACTATGTGCATCGGTGTCGGGCAGGGCATCGCCATGACTCTGGAGCGTGTTTGAGCATATCAGCCTGCGAGTACCCTACAATGATAACCaa                               <  2:2835310/92‑1 (MQ=255)
                                                                   gCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCaa                               <  2:3580335/100‑1 (MQ=255)
                                                                    cATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAAt                              >  1:2437267/1‑100 (MQ=255)
                                                                     aTTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATa                             <  2:1543669/100‑1 (MQ=255)
                                                                      ttGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATAc                            <  2:3706268/100‑1 (MQ=255)
                                                                        gtgCACCATGTGCATGGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACaa                          <  1:4064746/100‑1 (MQ=255)
                                                                          gCACGATGTGCATTGGTGTCGGTCAGGGCATCGCCATGGTTCTGAGGCGTGGTTGAGGATATCAGCCTGCGAGTACCCTACAATGATAACCAATACAAAg                        >  2:1427924/1‑100 (MQ=255)
                                                                          gCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAg                        >  1:3527037/1‑100 (MQ=255)
                                                                          gCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAg                        >  1:3986193/1‑100 (MQ=255)
                                                                           cACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGc                       <  1:3659141/100‑1 (MQ=255)
                                                                            aCCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAATCTGCGAGTACCCTACAATGATAACCAATACAAAGCt                      >  1:2901127/1‑100 (MQ=255)
                                                                               aTGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGa                   <  2:1864266/100‑1 (MQ=255)
                                                                                  tgCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAccc                <  2:3854201/100‑1 (MQ=255)
                                                                                  tgCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAccc                >  1:2547292/1‑100 (MQ=255)
                                                                                   gCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGGGTTTGAGCATATCAACCTGCGAGTACCCTAAAATGATAACCAATACAAAGCTTGACCCg               >  2:1447489/1‑100 (MQ=255)
                                                                                    cATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGa              >  1:1505898/1‑100 (MQ=255)
                                                                                    cATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGa              >  1:2970916/1‑100 (MQ=255)
                                                                                     aTCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAt             >  2:2702075/1‑100 (MQ=255)
                                                                                     aTCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAt             <  2:1512134/100‑1 (MQ=255)
                                                                                     aTCGGTGTCGGGCAGAGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCCAGTACTCTACAATGATAACCAATACAATGCTTGACCCGAt             <  2:3482430/100‑1 (MQ=255)
                                                                                      tCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt            >  1:696027/1‑100 (MQ=255)
                                                                                       cGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg           <  2:651244/100‑1 (MQ=255)
                                                                                       cGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg           <  1:2666959/100‑1 (MQ=255)
                                                                                       cGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTCAGCATATCAACCTGCGAGTACCCTACAATGCTAACCAATACAAAGCTTGACCCGATTg           <  2:382825/100‑1 (MQ=255)
                                                                                        ggTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGa          >  1:1326821/1‑100 (MQ=255)
                                                                                           gtCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAAc       <  2:2806378/100‑1 (MQ=255)
                                                                                                tCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGt  >  1:3259286/1‑100 (MQ=255)
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GGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGT  >  NC_000913/1461969‑1462164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: