Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,493,774 A→G 27.2% L435L (TTA→TTG trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,7740AG27.2% 217.6 / 99.4 143L435L (TTA→TTGtrgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (43/61);  new base G (12/27);  total (55/88)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 146 total aligned reads displayed.

GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCC  >  NC_000913/1493677‑1493873
                                                                                                 |                                                                                                   
gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAAt                                                                                                   >  2:2623141/1‑100 (MQ=255)
gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAAt                                                                                                   >  1:3697154/1‑100 (MQ=255)
 aTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGGATCAGTCAGGTTAAtt                                                                                                  <  2:936178/100‑1 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                                 <  1:3617211/100‑1 (MQ=255)
   ttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGa                                                                                                <  2:2108297/100‑1 (MQ=255)
    tGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGAc                                                                                               >  2:2380093/1‑100 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGAcc                                                                                              >  1:2647720/1‑100 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGAcc                                                                                              <  2:2247296/100‑1 (MQ=255)
      ccGCTGTCGCCAGCGAATTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCACTCAGGTTAATTGAcc                                                                                              <  2:563626/99‑1 (MQ=255)
       cGTTGTCGCCAGGGAAGTAAGGACTCTCGGAAGTCGCAGCGTTCAGGCTGCGAATGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAcc                                                                                              <  2:3500427/98‑1 (MQ=255)
       cGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTg                                                                                            >  2:31285/1‑100 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTgg                                                                                           <  1:337646/100‑1 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTgg                                                                                           >  2:2603429/1‑100 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTgg                                                                                           <  1:3860378/100‑1 (MQ=255)
          tGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTCAATTAACCTgact                                                                                         <  1:3559279/100‑4 (MQ=255)
           gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAGTCAGTCAGGTTAATTGACCTGGGGt                                                                                        >  2:184929/1‑100 (MQ=255)
           gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGt                                                                                        <  1:3906578/100‑1 (MQ=255)
           gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGt                                                                                        >  1:3160645/1‑100 (MQ=255)
            tCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTc                                                                                       >  2:3255482/1‑100 (MQ=255)
                 aGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCCGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATg                                                                                  <  1:2916193/100‑1 (MQ=255)
                  gCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGa                                                                                 >  2:3346335/1‑100 (MQ=255)
                    gAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAgg                                                                               <  1:770763/100‑1 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGt                                                                              <  1:3445181/100‑1 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGt                                                                              <  1:85578/100‑1 (MQ=255)
                      aGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTg                                                                             <  1:4034644/100‑1 (MQ=255)
                      aGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTg                                                                             <  1:969259/100‑1 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTgg                                                                            <  1:3686600/100‑1 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTgg                                                                            <  1:1985579/100‑1 (MQ=255)
                        tACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGc                                                                           <  2:3543797/100‑1 (MQ=255)
                        tACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGc                                                                           <  2:2326947/100‑1 (MQ=255)
                         aCGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGc                                                                           <  2:3921696/99‑1 (MQ=255)
                         aCGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCa                                                                          >  1:1592621/1‑100 (MQ=255)
                           ggACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAAc                                                                        >  1:3070048/1‑100 (MQ=255)
                           ggACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAAc                                                                        <  1:1886899/100‑1 (MQ=255)
                            gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAAc                                                                        >  1:619610/1‑99 (MQ=255)
                            gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACg                                                                       >  1:2951409/1‑100 (MQ=255)
                              cacTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGc                                                                     <  2:3070029/100‑1 (MQ=255)
                               acTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGcc                                                                    <  2:1798801/100‑1 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                                   >  2:1877847/1‑100 (MQ=255)
                                 tCGCAAGTCGCAGCGCTCAGTCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGGTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCgg                                                                  >  2:1374434/1‑100 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                 <  1:886836/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                 <  1:10990/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                 <  1:2926321/100‑1 (MQ=255)
                                   gCAAGTCGCAGCGCTCAGGAAGCGAGAGTGTTTGAAGGCTGGATAAGTGAATCAGTCAGGTTAATTTACCTGGGGTCGGATAAGGTGGCAACGGCCggg                                                                 <  2:2797749/99‑1 (MQ=255)
                                         cGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCa                                                          >  1:3986613/1‑100 (MQ=255)
                                         cGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCa                                                          <  1:788359/100‑1 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                         >  1:230588/1‑100 (MQ=255)
                                            aGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGa                                                       <  2:345962/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                     <  1:960552/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                     <  1:1534239/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                     <  2:3127730/100‑1 (MQ=255)
                                               gcTGAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                        <  2:3843285/96‑1 (MQ=255)
                                                  cAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTa                                                 >  1:2712660/1‑100 (MQ=255)
                                                  cAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTa                                                 >  2:643411/1‑100 (MQ=255)
                                                    ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt                                               >  1:864080/1‑100 (MQ=255)
                                                    ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt                                               >  1:3357552/1‑100 (MQ=255)
                                                     gcggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttg                                              >  2:521260/1‑100 (MQ=255)
                                                        gcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                           <  1:1183102/100‑1 (MQ=255)
                                                          gAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCCGGAAAACCATGAGCACTATTGTTGAt                                         <  1:2518518/100‑1 (MQ=255)
                                                           aaaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATg                                        <  2:908253/100‑1 (MQ=255)
                                                           aaaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATg                                        <  1:1700557/100‑1 (MQ=255)
                                                            aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAAGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGc                                       >  2:2479448/1‑100 (MQ=255)
                                                             agagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGcc                                      <  1:2476195/100‑1 (MQ=255)
                                                               agaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGt                                    >  2:1273584/1‑100 (MQ=255)
                                                                  ttGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                 >  1:1698770/1‑100 (MQ=255)
                                                                  ttGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                 <  1:442807/100‑1 (MQ=255)
                                                                   tGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCTACGGCCGGGACAACCATGAGCACTATTGTTGATGCCGTcgtg                                <  1:2791587/100‑3 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                 >  2:2694503/1‑98 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGa                               >  2:2992940/1‑100 (MQ=255)
                                                                     aaGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAg                              <  1:2706501/100‑1 (MQ=255)
                                                                      aGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCAGGGGTGGGATGAGGTGGCAACGGCCGGGAAAACCATGGGCACTATTGTTGATGCCGTCGCGAg                              >  1:2079487/1‑99 (MQ=255)
                                                                         cTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                          <  1:3690199/100‑1 (MQ=255)
                                                                          ttGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTca                         <  2:3197358/100‑1 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                       >  2:2684766/1‑100 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                       <  1:446055/100‑1 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                       <  1:3317814/100‑1 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                          >  2:1223654/1‑97 (MQ=255)
                                                                             aTCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac                      <  1:163528/100‑1 (MQ=255)
                                                                              tCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacaca                     <  2:818476/100‑1 (MQ=255)
                                                                                 gTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACatat                  >  2:1670886/1‑100 (MQ=255)
                                                                                  tGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACataac                 <  1:2825209/100‑3 (MQ=255)
                                                                                   gAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCa                <  2:2439895/100‑1 (MQ=255)
                                                                                    aaTCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCAt               >  1:2616758/1‑100 (MQ=255)
                                                                                    aaTCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCAt               >  1:2350773/1‑100 (MQ=255)
                                                                                    aaTCAGTCAGGTTAAGTGGACTGGGGTCGGATGAGGTGGCAACGGGCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATcgt               <  2:670670/100‑3 (MQ=255)
                                                                                     aTCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATg              >  1:3434644/1‑100 (MQ=255)
                                                                                       cagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc             <  2:3182136/99‑1 (MQ=255)
                                                                                       cagtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa            <  1:3400746/100‑1 (MQ=255)
                                                                                        agtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg           <  1:633693/100‑1 (MQ=255)
                                                                                        agtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg           >  2:1389971/1‑100 (MQ=255)
                                                                                        agtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg           <  2:2306534/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg          <  1:214683/100‑1 (MQ=255)
                                                                                         gtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg          <  2:23818/100‑1 (MQ=255)
                                                                                         gtcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg          >  1:219989/1‑100 (MQ=255)
                                                                                          tcagGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGa         <  2:2920658/100‑1 (MQ=255)
                                                                                            agGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGaaa       <  1:1600243/100‑1 (MQ=255)
                                                                                             ggTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt      <  2:1611290/100‑1 (MQ=255)
                                                                                               ttAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg    <  2:797587/100‑1 (MQ=255)
                                                                                                 aaTTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcgcc  <  1:164024/100‑1 (MQ=255)
                                                                                                 aaTTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcgc   >  2:270446/1‑99 (MQ=255)
                                                                                                 |                                                                                                   
GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCC  >  NC_000913/1493677‑1493873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: